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Histone Acetyl- & Methyl-Transferases / Related Products
You are here: Product Lines > DNA Regulation / Transcription > Histones & Histone Modification > Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-201-279 Revised 18-Mar-08
CREB Binding Protein (mouse) (recombinant) (His)
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SYNONYMS CBP (mouse) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY CREB & CREB Binding Protein / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-279-C002   2 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.3.1.48
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~50-100ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P45481: CREB Binding Protein (mouse)
Further Categories Containing This Product:
Histone Acetyl- & Methyl-Transferases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-280 Revised 18-Mar-08
GCN5 (human) (recombinant) (His)
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SYNONYMS GCN5L2 (human) (recombinant) (His)
Histone Acetyltransferase GCN5 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-201-280-C002   2 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.3.1.48
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~200-300ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q92830: GCN5 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-281 Revised 13-Aug-08
PCAF (mouse) (recombinant) (His)
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SYNONYMS P300/CBP-associated Factor (mouse) (recombinant) (His)
Histone Acetyltransferase PCAF (mouse) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-201-281-C020   20 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.3.1.48
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~100-200ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9JHD1: PCAF (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-283 Revised 18-Mar-08
CARM1 (human) (recombinant) (His)
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SYNONYMS Coactivator-associated Arginine Methyltransferase 1 (human) (recombinant) (His)
PRMT4 (human) (recombinant) (His)
Protein Arginine N-Methyltransferase 4 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-201-283-C002   2 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.1.1.125
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~50-100ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression: M. Covic, et al.; EMBO J. 24, 85 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q86X55: CARM1 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-210-287 Revised 13-Jan-05
Polyclonal Antibody to RbAp46 (human)
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SYNONYMS anti-Retinoblastoma Suppressor (Rb)-Associated Protein 46 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-210-287-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-13 (M1ASKEMFEDT
VEE13C) of human RbAp46 (retinoblastoma suppressor (Rb)-associated protein 46).
SPECIFICITY: Recognizes human RbAp46. Detects a band of ~46kDa by Western blot.
APPLICATION: Immunohistochemistry (1µg/ml)
Western Blot (0.5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-166-006.
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Product Description
Rb Associated Protein 46, RbAp46, is a nuclear protein that has been shown to interact directly with retinoblastoma protein, Rb, and is a component of the mSin3 corepressor complex as are the histone deactylase proteins (HDACs). RbAp46 shares about 75% sequence identity with the closely related RbAp48, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp46 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-288 Revised 13-Jan-05
Polyclonal Antibody to RbAp48 (human)
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SYNONYMS anti-Retinoblastoma Suppressor (Rb)-Associated Protein 48 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-210-288-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-14 (M1ADKFAAFDDA
VEE14C) of human RbAp48 (retinoblastoma suppressor (Rb)-associated protein 48).
SPECIFICITY: Recognizes human RbAp48. Detects a band of ~48kDa (and an unspecified band at ~60kDa) by Western blot.
APPLICATION: Western Blot (1:2'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-166-007.
Product Description
Rb Associated Protein 48, RbAp48, is a nuclear protein that has been shown to interact directly with retinoblastoma protein, Rb, and is a component of the mSin3 corepressor complex as are the histone deactylase proteins (HDACs). RbAp48 shares about 75% sequence identity with the closely related RbAp46, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp48 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp48 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp48 have shown strong inhibition of growth.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-270-381 Revised 28-Sep-07
Anacardic acid
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SYNONYMS 2-Hydroxy-6-pentadecylbenzoic acid
6-Pentadecylsalicylic acid
AA
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-270-381-M005   5 mg 40.00 USD Add To Cart
ALX-270-381-M025   25 mg 140.00 USD Add To Cart
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Product Specification
FORMULA: C22H36O3
MW: 348.5
MERCK INDEX: 14: 621
SOURCE/HOST: Synthetic.
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in 100% ethanol, methanol, DMSO, dichloromethane or ethylacetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: Protect from light. After reconstitution, prepare aliquots and store at -20°C. Packaged under inert gas.

Product Description
Cell permeable salicylic acid analog that acts as a potent, non-competitive inhibitor of p300 and PCAF (p300/CBP-associated factor) histone acetyltransferase (HAT) activities (IC50~8.5µM and ~5µM, respectively). Displays a variety of other biological activities, such as antibacterial, antimicrobial, prostaglandin synthase inhibition, tyrosinase, and lipoxygenase inhibition.
Product Specific Literature References
Anacardic Acid: molluscicide in cashew nut shell liquid: J.T. Sullivan, et al.; Planta Med. 44, 175 (1982) Abstract
Inhibition of lipoxygenase and prostaglandin endoperoxide synthase by anacardic acids: R. Grazzini, et al.; BBRC 176, 775 (1991) Abstract
Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin-resistant Staphylococcus aureus: H. Muroi & I. Kubo; J. Appl. Bacteriol. 80, 387 (1996) Abstract
Small molecule modulators of histone acetyltransferase p300: K. Balasubramanyam, et al.; J. Biol. Chem. 278, 19134 (2003) Abstract; Full Text
Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation: Y. Sun, et al.; FEBS Lett. 580, 4353 (2006) Abstract
General Literature References
Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin-resistant Staphylococcus aureus: H. Muroi and I. Kubo; J. Appl. Bacteriol. 80, 387 (1996) Abstract
Synthesis of sildenafil analogues from anacardic acid and their phosphodiesterase-5 inhibition: R. Paramashivappa, et al.; J. Agric. Food Chem. 50, 7709 (2002) Abstract
Further Categories Containing This Product:
Prostaglandin Synthases / Related Products • Lipoxygenases / Related Products • Antibiotics Other Products
 
 
ALX-270-411 Revised 01-Apr-08
Butyrolactone 3
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SYNONYMS Gcn5 Inhibitor 1
MB-3
3-Methylene-4-hydroxycarbonyl-5-(1-propyl)-tetrahydrofuran-2-one
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-270-411-M005   5 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C9H12O4
MW: 184.2
CAS NUMBER: 778649-18-6
SOURCE/HOST: Synthetic.
PURITY: ≥95% (1H-NMR)
APPEARANCE: White solid.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions in DMSO are stable for at least 2 months when stored at -20°C.

Product Description
Racemic. Inhibitor of human histone acetyltransferase Gcn5.
Product Specific Literature References
Design, synthesis, and biological evaluation of a small-molecule inhibitor of the histone acetyltransferase Gcn5: M. Biel, et al.; Angew. Chem. Int. Ed. Engl. 43, 3974 (2004) Abstract
 
 
ALX-270-473 Revised 13-Jun-08
BIX 01294
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SYNONYMS 2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-dimethoxy-N-[1-(phenylmethyl)-4-piperidinyl]4-quinazolinamine
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-270-473-M002   2 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C28H38N6O2
MW: 490.6
CAS NUMBER: 935693-62-2
PURITY: ≥95%
APPEARANCE: Light brown to brown solid.
SOLUBILITY: Soluble in DMSO (5mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
IDENTITY: Determined by 1H-NMR, 13C-NMR and MS.

Product Description
Selective and cell permeable inhibitor of G9a histone methyltransferase.
Product Specific Literature References
Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase: S. Kubicek, et al.; Mol. Cell 25, 473 (2007) Abstract
 
 
ALX-420-033 Revised 04-Jun-07
CTPB
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SYNONYMS N-(4-Chloro-3-trifluoromethyl-phenyl)-2-ethoxy-6-pentadecyl-benzamide
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-420-033-M001   1 mg 40.00 USD Add To Cart
ALX-420-033-M005   5 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C31H43ClF3NO2
MW: 554.1
CAS NUMBER: 586976-24-1
PURITY: ≥97% (1H-NMR)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Potent activator of p300 HAT (histone acetyltransferase), but not of PCAF (p300/CBP-associated factor) HAT activities.
General Literature References
Small molecule modulators of histone acetyltransferase p300: K. Balasubramanyam, et al.; J. Biol. Chem. 278, 19134 (2003) Abstract
 
 
ALX-850-326 Revised 07-Jan-08
HAT Activity Assay Kit
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-850-326-KI01   1 Kit 413.00 USD Add To Cart
Product Specification
QUANTITY: ~100 tests.
KIT/SET CONTAINS: 2x HAT Assay Buffer
HAT Substrate I
HAT Substrate II
NADH Generating Enzyme
NE (Nuclear Extract 4 mg/ml)
HAT Reconstitution Buffer
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Images
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Product Description
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Product Specific Literature References
Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells: P.A. Ruiz, et al.; J. Nutr. 137, 1208 (2007)