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ALX-210-533
Revised 31-Oct-06
Polyclonal Antibody to NF-AT5 (human)
SYNONYMS
anti-Nuclear Factor of Activated T Cells 5 (human) PAb
anti-NFAT5 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Nuclear Factor of Activated T Cells [NF-AT]
Ordering Information
Product Numbers:
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ALX-210-533-C050
50 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 1439-1455 (CD
1439
LLVSLQNQGNNLTGSF
1455
) of human NF-AT5 (nuclear factor of activated T cells 5). This sequence is 94% conserved in mouse NF-AT5.
SPECIFICITY:
Recognizes human NF-AT5. Detects a band of ~170kDa by Western blot.
APPLICATION:
Immunocytochemistry (1:1'000)
Western Blot (1:2’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Note:
Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells 5 (NF-AT5)
Gene Names: NFAT5
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-539
Revised 27-Oct-06
Polyclonal Antibody to XRCC1 (human)
SYNONYMS
anti-X-ray Repair Cross-complementing Group 1 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
XRCC Proteins / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-210-539-R100
100 µl
250.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum containing 0.1% sodium azide.
IMMUNOGEN:
Recombinant human XRCC1 (X-ray repair cross-complementing group 1) fused to a His-tag.
SPECIFICITY:
Recognizes human XRCC1.
APPLICATION:
Immunocytochemistry (1:1’000)
Immunoprecipitation (1:200)
Western Blot (1:2’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least one year when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage:
M. Masson, et al.; Mol. Cell. Biol.
18
, 3563 (1998)
Abstract
;
Full Text
XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage:
N. Levy, et al.; Nucl. Acids Res.
34
, 32 (2006)
Full Text
Related Products
ALX-210-304
Polyclonal Antibody to XRCC1 (human)
Further Categories Containing This Product:
PARP Other Products
•
Polyclonal Antibodies
ALX-210-540
Revised 07-Dec-06
Polyclonal Antibody to PARP-1 (BRCT Domain)
SYNONYMS
anti-Poly(ADP-ribose) Polymerase-1 (BRCT Domain) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
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Format:
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ALX-210-540-R100
100 µl
250.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Ammonium sulfate precipitate.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human PARP-1 (BRCT domain) (aa 385-524) fused to a His-tag (Prod. No.
ALX-201-255
).
SPECIFICITY:
Recognizes human and mouse PARP-1.
APPLICATION:
Immunoprecipitation (1:200)
Western Blot (1:8’000)
Does not work for immunocytochemistry.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least one year when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Related Products
ALX-201-255
PARP-1 (BRCT Domain) (human) (recombinant) (His)
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-541
Revised 13-May-08
Polyclonal Antibody to PARP-3
SYNONYMS
anti-Poly(ADP-ribose) Polymerase-3 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-3
Ordering Information
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ALX-210-541-R100
100 µl
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. Contains 0.05% sodium azide.
IMMUNOGEN:
Recombinant human PARP-3.
SPECIFICITY:
Recognizes human, mouse and monkey PARP-3.
APPLICATION:
Immunocytochemistry (1:200)
Immunoprecipitation (5μg for 500μg total proteins)
Western Blot (1:200-1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression:
A. Augustin, et al.; J. Cell Sci.
116
, 1551 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-550
Revised 08-Dec-04
Polyclonal Antibody to 8-Oxoguanine-DNA glycosylase (human)
SYNONYMS
anti-OGG1 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Repair Other Products
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ALX-210-550-R100
100 µl
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum in stabilization buffer containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human OGG1 (8-oxoguanine-DNA glycosylase).
SPECIFICITY:
Recognizes human OGG1. Detects a band of ~38kDa by Western blot.
APPLICATION:
Western Blot (in highly expressing cells; 1:500-1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
7,8-Dihydro-8-oxoguanine (8-oxoG, 8-hydroxyguanine) and 2,6-diamino-4-hydroxyformamidopyrimidine (Fapy) are major DNA lesions formed by reactive oxygen species and are involved in mutagenic and/or lethal events in cells. 8-Oxoguanine DNA glycoxylase (OGG1) can recognize both lesions end excise the altered base, leaving behind an abasic site that is restored to guanine by the cellular abasic site repair/replication machinery. Loss of OGG1 activity results in an overal G·C to T·A transversion mutation. The human OGG1 (hOGG1) gene is located on chromosome 3 (3p26), being subject to monoallelic deletion resulting in loss of heterozygosity. This state can be found in 100% of samll-cell lung cancers and up to 50% in other form of cancer. hOGG1 exists in two main splice variants, α-hOGG1 located in the nucleus and β-hOGG1 located in the mitochondrion. OGG1 was cloned initially from
S. cerevisiae
and recently from human as hOGG1 (hOGH1, hMMH).
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-551
Revised 23-Feb-05
Polyclonal Antibody to O6-Methylguanine-DNA Methyltransferase
SYNONYMS
anti-O
6
-Methylguanine-DNA Methyltransferase PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Repair Other Products
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ALX-210-551-R100
100 µl
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Contains 0.05% sodium azide.
IMMUNOGEN:
Human MGMT (O
6
-methylguanine-DNA methyltransferase).
SPECIFICITY:
Recognizes human, mouse and rat MGMT.
APPLICATION:
Western Blot (1:100-1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Specific Literature References
O6-methylguanine-DNA methyltransferase activity in breast and brain tumors:
I. Preuss, et al.; Int. J. Cancer
61
, 321 (1995)
Abstract
General Information
O
6
-Methylguanine-DNA methyltransferase (MGMT) removes O
6
-methylguanine from DNA by transferring the O
6
-alkyl group to a specific cysteine residue within its own sequence irreversibly inactivating MGMT. Unrepaired alkylated guanine will cause G·C -> A·T transition mutations by anomalous base pairing during DNA replication. DNA damage by O
6
-methylguanine can induce apoptosis regulated by Bcl-2 and caspases- 3 & -9. MGMT is able to remove O
4
-alkylated-thymine.
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products
•
Polyclonal Antibodies
ALX-210-552
Revised 31-Oct-06
Polyclonal Antibody to Apurinic/Apyrimidinic Endonuclease
SYNONYMS
anti-APE1 PAb
anti-APEX PAb
anti-HAP1 PAb
anti-Ref-1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
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Format:
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ALX-210-552-R100
100 µl
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Purified. Contains 0.05% sodium azide.
IMMUNOGEN:
Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY:
Recognizes human, mouse and rat APE1. Detects a band of ~37kDa by Western blot.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Description
Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5' of an AP site to produce a hydroxyl group at the 3' terminus of an unmodified nucleotide upstream of the nick and a 5'-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5'-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3'-5' DNA exonuclease activity, a 3'-phosphodiesterase activity, a 3'-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
Product Specific Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation:
S. Grosch, et al.; Cancer Res.
58
, 4410 (1998)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-553
Revised 22-Sep-06
Polyclonal Antibody to DNA Polymerase β
SYNONYMS
anti-Pol β PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Repair Other Products
Ordering Information
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ALX-210-553-R100
100 µl
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. 100µl of antiserum in stabilization buffer containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human Pol β (DNA polymerase β).
SPECIFICITY:
Recognizes human and mouse Pol β. Detects a band of ~45kDa by Western blot.
APPLICATION:
Western Blot (1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Description
During base excision repair (BER) DNA polymerase β (Pol β) is recruited by APE1. Pol β has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol β also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol β plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol β, as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol β does not excise 5'-oxidized AP sites induced by reactive oxygen species (ROS).
Product Specific Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation:
S. Grosch, et al.; Cancer Res.
58
, 4410 (1998)
Abstract
Cells deficient in DNA polymerase beta are hypersensitive to alkylating agent-induced apoptosis and chromosomal breakage:
K. Ochs, et al.; Cancer Res.
59
, 1544 (1999)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-559
Revised 31-Oct-06
Polyclonal Antibody to TetR
SYNONYMS
anti-Tetracycline Repressor PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcriptional Repressors & Corepressors / Related Products
Ordering Information
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ALX-210-559-R100
100 µl
280.00 USD
Product Specification
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Neat serum in PBS containing 50% glycerol and traces of sodium azide.
IMMUNOGEN:
Recombinant TetR (tetracycline repressor).
SPECIFICITY:
Recognizes the TetR portion of bait fusion protein in the yeast two-hybrid system. Detects a band of ~37kDa by Western blot.
APPLICATION:
Western Blot (1:500)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
This antibody can also be excellently used in RNAi expression systems relying on TetR regulation, by verifying the TetR expression levels. Double-check your cell-line if it is suitable for inducible, tet-repressor dependent RNAi expression.
Further Categories Containing This Product:
Gene Expression & Analysis Other Products
•
Polyclonal Antibodies
ALX-210-570
Revised 31-Oct-06
Polyclonal Antibody to Polycomb 2
SYNONYMS
anti-Pc2 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones & Histone Modification Other Products
Ordering Information
Product Numbers:
Format:
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ALX-210-570-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.5mg/ml.
PURITY:
Liquid. In PBS.
IMMUNOGEN:
Recombinant N-terminal part of human Pc2 (polycomb 2) (aa 1-228).
SPECIFICITY:
Recognizes human and mouse Pc2.
APPLICATION:
Immunoprecipitation
Western Blot (2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Literature References
Structure and catalytic mechanism of the human histone methyltransferase SET7/9
:
B. Xiao, et al.; Nature
421
, 652 (2003)
Abstract
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet
:
T. Kwon, et al.; EMBO J.
22
, 292 (2003)
Abstract
Further Categories Containing This Product:
Transcriptional Repressors & Corepressors / Related Products
•
Polyclonal Antibodies
ALX-210-574
Revised 18-Oct-07
Polyclonal Antibody to NF-κB (p65)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers:
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ALX-210-574-R100
100 µl
280.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
