ALEXIS Biochemicals: Rocaglaol

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ALX-804-022

Revised 26-Sep-08

Monoclonal Antibody to NF-ATc1 (7A6)
SYNONYMS	anti-Nuclear Factor of Activated T Cells, Cytoplasmic 1 MAb (7A6) anti-NFAT2 MAb (7A6) anti-NFATC MAb (7A6)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-022-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
CLONE:	7A6
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human NF-ATc1 (nuclear factor of activated T cells, cytoplasmic 1) (aa 1-654).
SPECIFICITY:	Recognizes human, mouse, rat and monkey NF-ATc1. Detects a band of ~105kDa by Western blot. Does not cross-react with NF-ATc2.
APPLICATION:	Flow Cytometry Gel Supershift Assay Immunocytochemistry (1:250) Immunoprecipitation, Chromatin Immunoprecipitation Western Blot (1:2’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity: S.N. Ho, et al.; J. Biol. Chem 270, 19898 (1995) Abstract; Full Text
Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells: K.M. Latinis, et al.; J. Biol. Chem. 272, 31427 (1997) Abstract; Full Text
Expression of NFAT-family proteins in normal human T cells: L. Lyakh, et al.; Mol. Cell. Biol. 17, 2475 (1997) Abstract; Full Text
The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol. 279, C915 (2000) Abstract; Full Text
The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W.J. Leonard; EMBO J. 21, 3051 (2002) Abstract; Full Text
Cannabinol enhancement of interleukin-2 (IL-2) expression by T cells is associated with an increase in IL-2 distal nuclear factor of activated T cell activity: T.R. Jan, et al.; Mol. Pharmacol. 61, 446 (2002) Abstract; Full Text
Induction of NFATc2 expression by interleukin 6 promotes T helper type 2 differentiation: S. Diehl, et al.; J. Exp. Med. 196, 39 (2002) Abstract; Full Text
Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells: C. Pfeifhofer, et al.; J. Exp. Med. 197, 1525 (2003) Abstract; Full Text
Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells: A. Kerstan & T. Hunig; J. Immunol. 172, 1341 (2004) Abstract
Active protein kinase B regulates TCR responsiveness by modulating cytoplasmic-nuclear localization of NFAT and NF-kappa B proteins: A.K. Patra, et al.; J. Immunol. 172, 4812 (2004) Abstract
IL-4-Induced GATA-3 Expression Is a Time-Restricted Instruction Switch for Th2 Cell Differentiation: N. Seki, et al.; J. Immunol. 172, 6158 (2004) Abstract
Differential roles for Wiskott-Aldrich syndrome protein in immune synapse formation and IL-2 production: J.L. Cannon and J.K. Burkhardt; J. Immunol. 173, 1658 (2004) Abstract; Full Text
Role of NFAT proteins in IL13 gene transcription in mast cells: S. Monticelli, et al.; J. Biol. Chem. 279, 36210 (2004) Abstract; Full Text
Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms: J. Barlic, et al.; J. Biol. Chem. 279, 48520 (2004) Abstract; Full Text
Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis: T. Minami, et al.; J. Biol. Chem. 279, 50537 (2004) Abstract; Full Text
NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching: K.J. McCullagh, et al.; PNAS 101, 10590 (2004) Abstract; Full Text
Rocaglamide derivatives are immunosuppressive phytochemicals that target NF-AT activity in T cells: P. Proksch, et al.; J. Immunol. 174, 7075 (2005) Abstract; Full Text
Vasopressin-dependent myogenic cell differentiation is mediated by both Ca2+/calmodulin-dependent kinase and calcineurin pathways: B.M. Scicchitano, et al.; Mol. Biol. Cell 16, 3632 (2005) Abstract; Full Text
Calcium-dependent activation of interleukin-21 gene expression in T cells: H.P. Kim, et al.; J. Biol. Chem. 280, 25291 (2005) Abstract; Full Text
Blockade of nuclear factor of activated T cells activation signaling suppresses balloon injury-induced neointima formation in a rat carotid artery model: Z. Liu, et al.; J. Biol. Chem. 280, 14700 (2005) Abstract; Full Text
Proteolytic regulation of nuclear factor of activated T (NFAT) c2 cells and NFAT activity by caspase-3: W. Wu, et al.; J. Biol. Chem. 281, 10682 (2006) Abstract; Full Text
Activity- and calcineurin-independent nuclear shuttling of NFATc1, but not NFATc3, in adult skeletal muscle fibers: T. Shen, et al.; Mol Biol Cell 17, 1570 (2006) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5)
Gene Names: NFAT5

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-215

Revised 24-Feb-06

Monoclonal Antibody to NF-ATc2 (25A10.D6.D2)
SYNONYMS	anti-Nuclear Factor of Activated T Cells, Cytoplasmic 2 MAb (25A10.D6.D2) anti-NFAT1 MAb (25A10.D6.D2) anti-NFATP MAb (25A10.D6.D2)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-215-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
CLONE:	25A10.D6.D2
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. Purified antibody in PBS containing 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 51-69 (A⁵¹ISSPSGLAYPDD VLDYGL⁶⁹) of mouse nuclear factor of activated T cells, cytoplasmic 2 (NF-ATc2) conserved in all three isoforms A, B and C.
SPECIFICITY:	Recognizes human, mouse and rat NF-ATc2. Detects bands of ~140kDa (phosphorylated NF-ATc2 in resting immune cells) and ~120kDa (fully-dephosphorylated NF-ATc2 in stimulated cells) by Western blot. Does not cross-react with NF-ATc1.
APPLICATION:	Gel Supershift Assays Immunocytochemistry (1µg/ml) Immunoprecipitation Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-163-009.

Product Specific Literature References

The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol. 279, C915 (2000) Abstract; Full Text
Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain: S. Plyte, et al.; J. Biol. Chem. 276, 14350 (2001) Abstract; Full Text
The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W. Leonard; EMBO J. 21, 3051 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5)
Gene Names: NFAT5

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-210-533

Revised 31-Oct-06

Polyclonal Antibody to NF-AT5 (human)
SYNONYMS	anti-Nuclear Factor of Activated T Cells 5 (human) PAb anti-NFAT5 (human) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-533-C050		50 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 1439-1455 (CD¹⁴³⁹LLVSLQNQGNNLTGSF¹⁴⁵⁵) of human NF-AT5 (nuclear factor of activated T cells 5). This sequence is 94% conserved in mouse NF-AT5.
SPECIFICITY:	Recognizes human NF-AT5. Detects a band of ~170kDa by Western blot.
APPLICATION:	Immunocytochemistry (1:1'000) Western Blot (1:2’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

BACKGROUND/TECHNICAL INFORMATION

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-210-577

Revised 26-May-08

Polyclonal Antibody to NF-ATc1
SYNONYMS	anti-Nuclear Factor of Activated T Cells, Cytoplasmic 1 PAb anti-NFAT2 PAb anti-NFATC PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-577-C050		50 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	500µg/ml
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Recombinant N-terminal human NF-ATc1 fragment expressed as GST fusion protein.
SPECIFICITY:	Recognizes human NF-ATc1. Shows some cross-reactivity with mouse NF-ATc1.
CROSSREACTIVITY PROTEINS:	May contain some residual anti-GST activity.
APPLICATION:	Immunoprecipitation and Chromatin Immunoprecipitation (1:200) Western Blot (1:500-1:1’000) Functional Application: Band Shift Assay: competes with probe for NFAT binding. The recommended dilutions refer to the analysis of cells and tissues with intermediate to high levels of NF-ATc1 and must be viewed as approximate. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Pim-1 kinase enhances NFATc activity and neuroendocrine functions in PC12 cells: M. Glazova, et al.; Brain Res. Mol. Brain Res. 138, 116 (2005) Abstract
Specific and redundant roles for NFAT transcription factors in the expression of mast cell-derived cytokines: M. Klein, et al.; J. Immunol. 177, 6667 (2006) Abstract; Full Text
Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling: A. Akimzhanov, et al.; Am. J. Pathol. 172, 215 (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-210-578

Revised 26-May-08

Polyclonal Antibody to NF-ATc2
SYNONYMS	anti-Nuclear Factor of Activated T Cells, Cytoplasmic 2 PAb anti-NFAT1 PAb anti-NFATP PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-578-C020		20 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	200µg/ml
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Recombinant human NF-ATc2 protein fused to GST.
SPECIFICITY:	Recognizes human NF-ATc2.
CROSSREACTIVITY PROTEINS:	May contain some residual anti-GST activity.
APPLICATION:	Immunohistochemistry (formaldehyde fixed paraffin sections: 1:50) Immunoprecipitation and Chromatin Immunoprecipitation (1:100) Western Blot (1:500) The recommended dilutions refer to the analysis of cells and tissues with intermediate to high levels of NF-ATc2 and must be viewed as approximate. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Specific and redundant roles for NFAT transcription factors in the expression of mast cell-derived cytokines: M. Klein, et al.; J. Immunol. 177, 6667 (2006) Abstract; Full Text
NFAT but not NF-kappaB is critical for transcriptional induction of the prosurvival gene A1 after IgE receptor activation in mast cells: E. Ulleras, et al.; Blood 111, 3081 (2008) Abstract
Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling: A. Akimzhanov, et al.; Am. J. Pathol. 172, 215 (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-210-532

Revised 31-Oct-06

Polyclonal Antibody to NF-ATc4 (human)
SYNONYMS	anti-Nuclear Factor of Activated T Cells, Cytoplasmic 4 (human) PAb anti-NFAT3 (human) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nuclear Factor of Activated T Cells [NF-AT]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-532-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 887-902 (CR⁸⁸⁷DLSGFPAPPGEEPPA⁹⁰²) of human NF-ATc4 (nuclear factor of activated T cells, cytoplasmic 4). This sequence is 94% conserved in mouse NF-ATc4.
SPECIFICITY:	Recognizes human NF-ATc4. Detects a band of ~150kDa by Western blot.
APPLICATION:	Immunohistochemistry (1:3’000) Western Blot (1:10’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

BACKGROUND/TECHNICAL INFORMATION

Further Categories Containing This Product:

Polyclonal Antibodies

ALX-350-121

Revised 14-Mar-08

Rocaglamide
SYNONYMS	Rocaglamide A
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - NF-kB Pathway Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-121-C100		100 µg	220.00 USD

Product Specification

FORMULA:	C₂₉H₃₁NO₇
MW:	505.6
CAS NUMBER:	84573-16-0
SOURCE/HOST:	Isolated from Aglaia sp.
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white sticky solid.
SOLUBILITY:	Soluble in DMSO, 100% ethanol or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Immunosuppressant. Potent inhibitor of NF-κB activation in T cells, with an almost complete inhibition at 200nM. Suppresses cytokine production (IFN-γ, TNF-α, IL-2 and IL-4) and inhibits NF-AT in peripheral blood T cells at concentrations that do not impair NF-κB and AP-1 activities. In contrast to the immunosuppressant cyclosporin A (Prod. No. ALX-380-002), rocaglamide does not inhibit calcineurin phosphatase activity. Induces apoptosis.

Product Specific Literature References

X-ray crystal structure of rocaglamide, a novel antileukemic 1H-cyclopenta-(b)-benzofuran from Aglaia elliptofolia: M.L. King, et al.; J. C. S. Chem. Commun. 20, 1150 (1982)
Cyclopenta[b]benzofurans from Aglaia species with pronounced antifungal activity against rice blast fungus (Pyricularia grisea): D. Engelmeier, et al.; J. Agric. Food Chem. 48, 1400 (2000)
Insecticidal rocaglamide derivatives from Aglaia spectabilis (Meliaceae): C. Schneider, et al.; Phytochemistry 54, 731 (2000)
New insecticidal rocaglamide derivatives and related compounds from Aglaia oligophylla: M. Dreyer, et al.; J. Nat. Prod. 64, 415 (2001)
Rocaglamides, glycosides, and putrescine bisamides from Aglaia dasyclada: Chaidir, et al.; J. Nat. Prod. 64, 1216 (2001) Abstract
Rocaglamide derivatives are potent inhibitors of NF-kappa B activation in T-cells: B. Baumann, et al.; J. Biol. Chem. 277, 44791 (2002) Abstract; Full Text
Rocaglamide derivatives are immunosuppressive phytochemicals that target NF-AT activity in T cells: P. Proksch, et al.; J. Immunol. 174, 7075 (2005) Abstract; Full Text
Potential of cyclopenta[b]benzofurans from Aglaia species in cancer chemotherapy: S. Kim, et al.; Anticancer Agents Med. Chem. 6, 319 (2006)
Potent cytotoxic rocaglamide derivatives from the fruits of Amoora cucullata: P. Chumkaew, et al.; Chem. Pharm. Bull. 54, 1344 (2006)
The traditional Chinese herbal compound rocaglamide preferentially induces apoptosis in leukemia cells by modulation of mitogen-activated protein kinase activities: J.Y. Zhu, et al.; Int. J. Cancer 121, 1839 (2007) Abstract

ALX-350-135	Rocaglaol
ALX-350-142	Rocaglamide I
ALX-350-141	Rocaglamide C
ALX-350-140	Rocaglamide AL
ALX-350-143	Rocaglamide J
ALX-380-002	Cyclosporin A

ALX-350-121	Rocaglamide
ALX-350-142	Rocaglamide I
ALX-350-143	Rocaglamide J
ALX-350-141	Rocaglamide C
ALX-350-135	Rocaglaol

FORMULA:	C₂₆H₂₄O₇
MW:	448.5
CAS NUMBER:	201212-33-1
SOURCE/HOST:	Isolated from Aglaia sp.
PURITY:	≥97% (HPLC)
APPEARANCE:	White to off-white sticky solid.
SOLUBILITY:	Soluble in DMSO or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
IDENTITY:	Identity determined by MS, ¹H-NMR.