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Transcription Factors/Related Products
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ALX-804-733 Revised 05-Feb-08
Monoclonal Antibody to Ribosomal Protein S3 (human) (RP159-1)
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SYNONYMS anti-RPS3 (human) MAb (RP159-1)
anti-40S Ribosomal Protein S3 (human) MAb (RP159-1)
anti-IMR-90 Ribosomal Protein S3 (human) MAb (RP159-1)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Ribosomal Proteins/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-733-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: RP159-1
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ribosomal protein S3.
SPECIFICITY: Recognizes human ribosomal protein S3.
APPLICATION: ELISA (direct and indirect: 1:500-1:5’000)
Western Blot (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation: T.S. Kim, et al.; Mol. Biol. Cell 17, 824 (2006) Abstract; Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK: S.H. Kim & J. Kim; Biochim. Biophys. Acta 1763, 823 (2006) Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation: F. Wan, et al.; Cell 131, 927 (2007) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins/Related Products • NF-kB Pathway Other Products • DNA Endonucleases/Related Products • DNA Endonucleases/Related Products • MAPK Pathway Other Products • Monoclonal Antibodies
 
 
ALX-400-047 Revised 24-Oct-07
Monosodium urate (crystals)
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SYNONYMS MSU (crystals)
Uric Acid (crystals)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-400-047-M002   2 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C5H4N4O3 . Na
MW: 168.1 . 23.0
CAS NUMBER: 1198-77-2
RTECS: YU7062000
CONCENTRATION: 5mg/ml after reconstitution.
PURITY DETAIL: Not determined. Degradation of the crystals with uricase (which breaks purines down into a very soluble product called allantoin) inhibits its activity.
RECONSTITUTION: Reconstitute with PBS.
ACTIVITY: Stimulates the caspase-1 activating NALP3 inflammasome, resulting in the production of active IL-1β and IL-18.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Vortex before use.

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Product Description
Specially crystallized and tested on biological activity.
Product Specific Literature References
Gout-associated uric acid crystals activate the NALP3 inflammasome: F. Martinon, et al.; Nature 440, 237 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Activation of THP-1 cells by uric acid:

1. THP-1 cells are cultured in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum
    (FCS), 50μM β-mercaptoethanol, 2mM L-glutamine and penicillin/streptomycin. THP-1 cells are grown
    in suspension to a density of 1.5x106 cells/ml in tissue culture flasks.
2. Treat THP-1 cells for 3 hours with 0.5µM PMA (Prod. No. ALX-445-004) the day before stimulation.
3. Wash cells with PBS and plate them in a 12-well plate at a density of 6x105 cells/well in RPMI medium
    (this increases the phagocytic properties of the cells and induces a constitutive production of proIL-1β).
4. The next day, wash cells with PBS and add 500µl Optimem (Invitrogen).
5. Stimulate the cells for 6 hours by addition of 50-200µg/ml of the suspension of uric acid (vortex the
    suspension just before use).
6. As a positive control, 5mM of ATP or 50µg/ml of LPS from E. coli, Serotype R515 (Re) (TLRgrade™)
    (liquid) (Prod. No. ALX-581-007) are incubated for 6 hours.

Detection of the mature IL-1β in the supernatant of uric acid-activated THP-1 cells:

1. Collect the supernatant of each well (500µl). Add 500µl methanol and 125µl chloroform.
2. Vortex and centrifuge for 5 min. at 13’000 rpm.
3. Remove the upper phase.
4. Add 500µl methanol and centrifuge for 5 min. at 13’000 rpm.
5. Remove the supernatant, dry the pellet for 5 min. at 50°C.
6. Add 60µl of 1x loading buffer and DTT (final concentration of 0.1M).
7. Mix and boil before loading on a 15% SDS-gel and transfer onto nitrocellulose membrane. The mature
    IL-1β is detected using the cleaved IL-1β (Asp116) antibody (Cell Signaling).

--> Note: Detection of IL-1β in the supernatant of uric acid-activated THP-1 cells can also be performed by ELISA.
--> Note: Sonication of MSU improves its biological activity in murine cells.
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ALX-385-016 Revised 02-Jun-08
Morin
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SYNONYMS 2’,3,4’,5,7-Pentahydroxyflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavonoids/Related Products
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ALX-385-016-G001   1 g 16.00 USD Add To Cart
Product Specification
FORMULA: C15H10O7
MW: 302.2
CAS NUMBER: 480-16-0
MERCK INDEX: 14: 6269
RTECS: LK8749000
PURITY: ≥85%
APPEARANCE: White to brown powder.
SOLUBILITY: Soluble in methanol or 100% ethanol; slightly soluble in ether or acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.

Product Description
Antioxidant flavonoid. Shows anti-proliferative and antitumor properties. Induces apoptosis. Induces cell cycle arrest at the G2/M phase. Anti-inflammatory compound. Suppresses NF-κB activation. Induces lipid peroxidation and DNA strand breaks.
Product Specific Literature References
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Lipid peroxidation and DNA damage induced by morin and naringenin in isolated rat liver nuclei: S. C. Sahu & G. C. Gray; Food Chem. Toxicol. 35, 443 (1997) Abstract
Morin inhibits the growth of human leukemia HL-60 cells via cell cycle arrest and induction of apoptosis through mitochondria dependent pathway: H.M. Kuo, et al.; Anticancer Res. 27, 395 (2007) Abstract
Morin (3,5,7,2’,4’-Pentahydroxyflavone) abolishes nuclear factor-kappaB activation induced by various carcinogens and inflammatory stimuli, leading to suppression of nuclear factor-kappaB-regulated gene expression and up-regulation of apoptosi: S.K. Manna, et al.; Clin. Cancer Res. 13, 2290 (2007) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • Natural Products - NF-kB Pathway Inhibitors • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Antitumor Reagents • Natural Products - Anti-inflammatory Agents • Natural Products for Cell Cycle Research • Natural Products - Antioxidants
 
 
ALX-385-012 Revised 19-Nov-07
Myricetin
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SYNONYMS 3,3',4',5,5',7-Hexahydroxyflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavonoids/Related Products
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ALX-385-012-M010   10 mg 35.00 USD Add To Cart
ALX-385-012-M050   50 mg 140.00 USD Add To Cart
Product Specification
FORMULA: C15H10O8
MW: 318.2
CAS NUMBER: 529-44-2
MERCK INDEX: 14: 6332
RTECS: LK8646000
PURITY: ≥97%
APPEARANCE: Light yellow-green crystals.
SOLUBILITY: Soluble in alcohol; slightly soluble in water (warm); insoluble in acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HANDLING: Protect from light.

Product Description
Antioxidant flavonoid. Has antitumor and chemopreventive properties. Anti-inflammatory. Inhibits NF-κB activation. 
Product Specific Literature References
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Biological effects of myricetin: K. C. Ong & H. E. Khoo; Gen. Pharmacol. 29, 121 (1997), (Review) Abstract
In vitro cytotoxicity of glyco-S-nitrosothiols. a novel class of nitric oxide donors: H. Babich & H.L. Zuckerbraun; Toxicol. In Vitro 15, 181 (2001) Abstract
Suppression of TNFalpha-mediated NFkappaB activity by myricetin and other flavonoids through downregulating the activity of IKK in ECV304 cells: S. H. Tsai, et al.; J. Cell Biochem. 74, 606 (1999) Abstract
Myricetin inhibits matrix metalloproteinase 2 protein expression and enzyme activity in colorectal carcinoma cells: C.H. Ko, et al.; Mol. Cancer Ther. 4, 281 (2005) Abstract
Mitochondrial-dependent, reactive oxygen species-independent apoptosis by myricetin: roles of protein kinase C, cytochrome c, and caspase cascade: C.H. Ko, et al.; Biochem. Pharmacol. 69, 913 (2005) Abstract
Inhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity: J. Lu, et al.; Cancer Res. 66, 4410 (2006) Abstract
Myricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1: K.W. Lee, et al.; Carcinogenesis 28, 1918 (2007) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • Natural Products - NF-kB Pathway Inhibitors • Natural Products - Antitumor Reagents • Natural Products - Anti-inflammatory Agents • Natural Products - Antioxidants • Active Substances from Fruit and Vegetables
 
 
ALX-840-603 Revised 08-Mar-07
Positive Control (Cell Lysate) for Antibodies to NALP1 (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to NACHT-, LRR- and PYD-containing Protein 1 (human)
Positive Control (Cell Lysate) for Antibodies to DEFCAP (human)
Positive Control (Cell Lysate) for Antibodies to NAC (human)
Positive Control (Cell Lysate) for Antibodies to CARD7 (human)
Positive Control (Cell Lysate) for Antibodies to Caspase Recruitment Domain-containing Protein 7 (human)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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ALX-840-603-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human NALP1 (aa 1-1429).
QUANTITY: 100µl corresponding to 2 x 106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to NALP1 (human) (Nalpy1-4) (Prod. No. ALX-804-803), PAb to NALP1 (human) (AL176) (Prod. No. ALX-210-904), PAb to NALP1 (human) (CT) (Bur 242) (Prod. No. ALX-210-017) and PAb to NALP1 (human) (NT) (Bur 241) (Prod. No. ALX-210-018).
Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products
 
 
ALX-163-011 Revised 14-Jul-08
NBD Peptide /  NF-κB Blocker (cell permeable)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-163-011-1   1 Vial 240.00 USD Add To Cart
Product Specification
SEQUENCE: GRKKRRQRRR-GG-TALDWSWLQTE-amide
FORMULA: C121H202N48O32
MW: 2841.2
QUANTITY: 50µl
CONCENTRATION: 10mM
PURITY: ≥95% (HPLC)
FORMULATION: Liquid. In sterile PBS.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Protect from moisture. Keep on ice at all times.
POSITIVE CONTROL: Prod. No. ALX-168-009/1
Product Description
For increased cell permeability the peptide was covalently linked to the 10 aa recognized by the TAT transporter.
Product Specific Literature References
Selective inhibition of NF-kappaB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo: E. Jimi, et al.; Nature Med. 10, 617 (2004) Abstract
Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex: M.J. May, et al.; Science 289, 1550 (2000) Abstract
Characterization of the Ikappa B-kinase NEMO binding domain: M.J. May, et al.; J. Biol. Chem. 277, 45992 (2002) Abstract
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Further Categories Containing This Product:
Peptides
 
 
ALX-201-285 Revised 13-Aug-08
NF-κB (p50) (human) (recombinant) (His)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors/Related Products
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ALX-201-285-C002   2 µg 280.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.2mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P19838: NF-κB (human) (p50 subunit)
AfCS Signalling Gateway link A002937: NF-κB (mouse) (p50 subunit)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-201-284 Revised 18-Mar-08
NF-κB (p65) (human) (recombinant) (His)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors/Related Products
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ALX-201-284-C002   2 µg 280.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression: M. Covic, et al.; EMBO J. 24, 85 (2005) Abstract; Full Text
MYBBP1a is a novel repressor of NF-κB: H. R. Owen, et al.; J. Mol. Biol. 366, 725 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q04206: NF-κB (human) (p65 subunit)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-380-050 Revised 20-Jun-08
Nigericin . sodium salt
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SYNONYMS Antibiotic K 178
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Ionophores
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ALX-380-050-M005   5 mg 50.00 USD Add To Cart
ALX-380-050-M025   25 mg 200.00 USD Add To Cart
Product Specification
FORMULA: C40H67O11 . Na
MW: 724.0 . 23.0
CAS NUMBER: 28380-24-1
MERCK INDEX: 14: 6541
SOURCE/HOST: Isolated from Streptomyces hygroscopicus.
PURITY: ≥98%
SOLUBILITY: Soluble in methylene chloride (10mg/ml), 100% ethanol or ethyl acetate; insoluble in water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for 1 month when stored at -20°C.
HAZARD: TOXIC.

Product Description

Acts as an H+, K+, Pb2+ ionophore. Is most commonly an antiporter of H+ and K+. Inhibits the Golgi functions.
Product Specific Literature References
Nigericin, a new crystalline antibiotic from an unidentified streptomyces: R. L. Harned, et al.; Antibiot. Chemother. 1, 594 (1951)
ntracellular processing and transport of influenza-virus envelope proteins in Madin-Darby canine kidney cells. Effects of the carboxylic ionophores monensin and nigericin: P.U. Daniels & J.M. Edwardson; Biochem. J. 252, 693 (1988) Abstract
Nigericin-induced Na+/H+ and K+/H+ exchange in synaptosomes: effect on [3H]GABA release: R. Rodriguez & M. Sitges; Neurochem. Res. 21, 889 (1996) Abstract
Nigericin inhibits accumulation of the steroidogenic acute regulatory protein but not steroidogenesis: S.R. King, et al.; Mol. Cell. Endocrinol. 166, 147 (2000) Abstract
Nigericin inhibits insulin-stimulated glucose transport in 3T3-L1 adipocytes: C.Y. Chu, et al.; J. Cell. Bioch