ALEXIS Biochemicals: Polyclonal Antibody to Polycomb 2

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ALX-210-246

Revised 14-Sep-06

Polyclonal Antibody to PER1 (mouse)
SYNONYMS	anti-Period Circadian Protein 1 (mouse) PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Transcriptional Repressors & Corepressors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-246-R200		200 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
FORMULATION:	Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 39-51 (P³⁹SLADDTDANSNG⁵¹) of mouse PER1 (period circadian protein 1).
SPECIFICITY:	Recognizes mouse PER1.
APPLICATION:	Immunohistochemistry (frozen and paraffin sections (1:500)) Western Blot (1:200)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-043.

Product Description

Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Tranasactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. PER, when complexed with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.

Further Categories Containing This Product:

Circadian Transcriptional Regulators / Related Products • Polyclonal Antibodies

ALX-210-559

Revised 31-Oct-06

Polyclonal Antibody to TetR
SYNONYMS	anti-Tetracycline Repressor PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Transcriptional Repressors & Corepressors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-559-R100		100 µl	280.00 USD

Product Specification

SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Neat serum in PBS containing 50% glycerol and traces of sodium azide.
IMMUNOGEN:	Recombinant TetR (tetracycline repressor).
SPECIFICITY:	Recognizes the TetR portion of bait fusion protein in the yeast two-hybrid system. Detects a band of ~37kDa by Western blot.
APPLICATION:	Western Blot (1:500) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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General Information

This antibody can also be excellently used in RNAi expression systems relying on TetR regulation, by verifying the TetR expression levels. Double-check your cell-line if it is suitable for inducible, tet-repressor dependent RNAi expression.

Further Categories Containing This Product:

Gene Expression & Analysis Other Products • Polyclonal Antibodies

ALX-210-570

Revised 31-Oct-06

Polyclonal Antibody to Polycomb 2
SYNONYMS	anti-Pc2 PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Histones & Histone Modification Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-570-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	0.5mg/ml.
PURITY:	Liquid. In PBS.
IMMUNOGEN:	Recombinant N-terminal part of human Pc2 (polycomb 2) (aa 1-228).
SPECIFICITY:	Recognizes human and mouse Pc2.
APPLICATION:	Immunoprecipitation Western Blot (2µg/ml) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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General Literature References

Structure and catalytic mechanism of the human histone methyltransferase SET7/9: B. Xiao, et al.; Nature 421, 652 (2003) Abstract
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet: T. Kwon, et al.; EMBO J. 22, 292 (2003) Abstract

Further Categories Containing This Product:

Transcriptional Repressors & Corepressors / Related Products • Polyclonal Antibodies

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