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DNA Fragmentation, Damage & Repair
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ALX-201-065 Revised 18-Sep-08
MGMT (human) (recombinant)
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SYNONYMS O6-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-065-R050   50 µl 320.00 USD Add To Cart
Product Specification
EC: 2.1.1.63
SOURCE/HOST: Produced in E. coli. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY: Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION: Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY: 100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O6-methylguanine from DNA.
QUALITY CONTROL: The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P16455: MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-077 Revised 10-Jul-07
DNase I-like Endonuclease 3 (human) (recombinant)
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SYNONYMS DNAS1L3 (human) (recombinant)
Deoxyribonuclease γ (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-201-077-C010   10 µg 350.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM potassium chloride and 50% glycerol.
APPLICATION: For studying DNAS1L3 catalyzed oligonucleosomal DNA fragmentation.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Specific Literature References
Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments: A.G. Yakovlev, et al.; Nucl. Acids Res. 27, 1999 (1999) Abstract
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase: A.G. Yakovlev, et al.; J. Biol. Chem. 275, 21302 (2000) Abstract; Full Text
Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation: A.H. Boulares, et al.; J. Biol. Chem. 277, 372 (2002) Abstract; Full Text
The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells: A.H. Boulares, et al.; Cancer Res. 62, 4439 (2002) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION For maximum activity, use at pH 7.5 with 2.5mM Ca2+ and 5mM Mg2+. Causes single strand nicks in duplex DNA and can be inhibited by Zn2+, but not by actin.
 
 
ALX-201-230 Revised 26-Apr-06
Endonuclease G (rat) (recombinant)
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SYNONYMS EndoG (rat) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY HtrA2 / Omi, ARTS, EndoG / Related Products
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ALX-201-230-C020   20 µg 190.00 USD Add To Cart
Product Specification
CONCENTRATION: 0.4mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Further Categories Containing This Product:
DNA Endonucleases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-244 Revised 27-Mar-07
Endonuclease G (human) (recombinant)
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SYNONYMS EndoG (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY HtrA2 / Omi, ARTS, EndoG / Related Products
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ALX-201-244-C020   20 µg 190.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Recombinant human endonuclease G is fused to a His-tag at the C-terminus.
CONCENTRATION: 0.4mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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Further Categories Containing This Product:
DNA Endonucleases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-249 Revised 18-Mar-08
FEN-1 (human) (recombinant)
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SYNONYMS Flap Structure-specific Endonuclease 1 (human) (recombinant)
MF1 (human) (recombinant)
Maturation Factor 1 (human) (recombinant)
DNase IV (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-201-249-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~42kDa
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 1mM DTT, 1mM EDTA and 0.02% Nonidet P40.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 4 weeks when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Flap endonuclease 1: a novel tumour suppresser protein: G. Henneke, et al.; TIBS 28, 384 (2003) Abstract
The Fen1 extrahelical 3’-flap pocket is conserved from archaea to human and regulates DNA substrate specificity: E. Friedrich-Heineken and U. Hubscher; Nucl. Acids Res. 32, 2520 (2004) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P39748: FEN-1 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-258 Revised 18-Mar-08
Lysyl Oxidase-like Protein 4 (human) (recombinant) (His)
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SYNONYMS LOXL4 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nucleic Acid Oxidation & Alkylation / Related Products
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ALX-201-258-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: ~65kDa
SOURCE/HOST: Produced in E. coli. Human lysyl oxidase-like protein 4 (LOXL4) (aa 159-756) is fused to a His-tag.
CONCENTRATION: ~0.33mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized from 50mM HEPES, pH 8.0, containing 200mM sodium chloride.
RECONSTITUTION: Reconstitute with 300µl of 10% glycerol containing 2mM β-mercaptoethanol.
SPECIFIC ACTIVITY: 0.02-0.03µmol/min/µg
APPLICATION: In vitro conjugation assays by monitoring benzylamine oxidation at A250mm. Typical concentration to support conjugation in vitro is 0.5-2.5µM depending on conditions.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
A novel human lysyl oxidase-like gene (LOXL4) on chromosome 10q24 has an altered scavenger receptor cysteine rich domain: L. Asuncion, et al.; Matrix Biol. 20, 487 (2001) Abstract
Cloning and characterization of a fifth human lysyl oxidase isoenzyme: the third member of the lysyl oxidase-related subfamily with four scavenger receptor cysteine-rich domains: J.M. Maki, et al.; Matrix Biol. 20, 493 (2001) Abstract
Lysyl oxidases: a novel multifunctional amine oxidase family: K. Csiszar; Prog. Nucl. Acid Res. Mol. Biol. 70, 1 (2001) Abstract
Molecular cloning and biological activity of a novel lysyl oxidase-related gene expressed in cartilage: H. Ito, et al.; J. Biol. Chem. 276, 24023 (2001) Abstract; Full Text
A molecular role for lysyl oxidase in breast cancer invasion: D.A. Kirschmann, et al.; Cancer Res. 62, 4478 (2002) Abstract; Full Text
Purification of enzymatically active human lysyl oxidase and lysyl oxidase-like protein from Escherichia coli inclusion bodies: S.T. Jung, et al.; Protein Expr. Purif. 31, 240 (2003) Abstract
Expression and purification of enzymatically active forms of the human lysyl oxidase-like protein 4: M.S. Kim, et al.; J. Biol. Chem. 278, 52071 (2003) Abstract; Full Text
Structural and functional diversity of lysyl oxidase and the LOX-like proteins: J. Molnar, et al.; Biochim. Biophys. Acta 1647, 220 (2003) Abstract
Lysyl oxidase is essential for hypoxia-induced metastasis: J.T. Erler, et al.; Nature 440, 1222 (2006) Abstract
Selective upregulation and amplification of the lysyl oxidase like-4 (LOXL4) gene in head and neck squamous cell carcinoma: T. Gorogh, et al.; J. Pathol. 212, 74 (2007) Abstract
LOXL1 and LOXL4 are epigenetically silenced and can inhibit ras/extracellular signal-regulated kinase signaling pathway in human bladder cancer: G. Wu, et al; Cancer Res. 67, 4123 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96JB6: Lysyl Oxidase-like Protein 4 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-276 Revised 05-Dec-07
DNA Polymerase λ (human) (recombinant)
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SYNONYMS Pol λ (human) (recombinant)
DNA Polymerase κ (human) (recombinant)
DNA Polymerase β2 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-276-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~64kDa
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 2.5mg/ml
PURITY: ≥98% (SDS-PAGE).
FORMULATION: Liquid. In 40mM TRIS/HCl, pH 7.5, containing 150mM NaCl, 1mM DTT, 1mM β-mercaptoethanol and 50% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for several months when stored at -20°C or -80°C.
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-277 Revised 24-Sep-07
Apurinic/Apyrimidinic Endonuclease (human) (recombinant)
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SYNONYMS APE1 (human) (recombinant)
APEX (human) (recombinant)
HAP1 (human) (recombinant)
Ref-1 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-201-277-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~36kDa
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Liquid. In 20mM HEPES-KOH, pH 7.5, containing 100mM NaCl and 20% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Enzyme is freshly produced upon customer request and is stable
for ~2 weeks at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair: A. Gembka, et al.; Nucleic Acids Res. 35, 2596 (2007) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-278 Revised 30-Sep-08
DNA Ligase I (human) (recombinant) (His)
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SYNONYMS DNA Ligase 1 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Replication Other Products
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ALX-201-278-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~102kDa. Note: The mobility of DNA ligase I in a PAA gel is not corresponding to its MW; it runs at ~125kDa.
SOURCE/HOST: Produced in E. coli. Full length human DNA ligase I is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.6mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM HEPES-KOH, pH 7.5, containing 30mM sodium chloride, 1mM EDTA, 1mM DTT and 30% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
USE/STABILITY: Enzyme is freshly produced upon customer request and is stable for ~2 weeks at -80°C. Very prone to degradation even in the absence of proteases. Freeze in liquid nitrogen for long term storage.
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair: A. Gembka, et al.; Nucleic Acids Res. 35, 2596 (2007) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Repair Other Products
 
 
ALX-201-295 Revised 20-Oct-08 New product
DNA Polymerase β (human) (recombinant)
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SYNONYMS Pol β (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-295-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~39kDa
EC: 2.7.7.7, 4.2.99.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.35mg/ml
PURITY: ≥98% (SDS-PAGE).
FORMULATION: Liquid. In 20mM TRIS, pH 7.5, containing 20% (v/v) glycerol, 1mM DTT and 100mM sodium chloride.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for more then 1 year when stored at -20°C or -80°C.
HANDLING: Avoid more than 5 freeze/thaw cycles.
Product Specific Literature References
Purification and domain-mapping of mammalian DNA polymerase beta: W.A. Beard & S.H. Wilson; Methods Enzymol. 262, 98 (1995) Abstract
General Information
During base excision repair (BER) DNA polymerase β (Pol β) is recruited by APE1. Pol β has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol β also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol β plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol β, as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol β does not excise 5’-oxidized AP sites induced by reactive oxygen species (ROS).
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P06746: DNA polymerase beta
General Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation: S. Grösch, et al.; Cancer Res. 58, 4410 (1998) Abstract; Full Text
Cells deficient in DNA polymerase beta are hypersensitive to alkylating agent-induced apoptosis and chromosomal breakage: K. Ochs, et al.; Cancer Res. 59, 1544 (1999) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-296 Revised 15-Oct-08 New product
Replication Protein A (human) (recombinant)
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SYNONYMS RP-A (human) (recombinant)
Replication Factor A (human) (recombinant)
RF-A (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-296-C005