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ALX-201-077
Revised 10-Jul-07
DNase I-like Endonuclease 3 (human) (recombinant)
SYNONYMS
DNAS1L3 (human) (recombinant)
Deoxyribonuclease γ (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-077-C010
10 µg
350.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM potassium chloride and 50% glycerol.
APPLICATION:
For studying DNAS1L3 catalyzed oligonucleosomal DNA fragmentation.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
Product Specific Literature References
Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments:
A.G. Yakovlev, et al.; Nucl. Acids Res.
27
, 1999 (1999)
Abstract
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase:
A.G. Yakovlev, et al.; J. Biol. Chem.
275
, 21302 (2000)
Abstract
;
Full Text
Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation
:
A.H. Boulares, et al.; J. Biol. Chem.
277
, 372 (2002)
Abstract
;
Full Text
The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells
:
A.H. Boulares, et al.; Cancer Res.
62
, 4439 (2002)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
For maximum activity, use at pH 7.5 with 2.5mM Ca
2+
and 5mM Mg
2+
. Causes single strand nicks in duplex DNA and can be inhibited by Zn
2+
, but not by actin.
ALX-201-094
Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-094-U025
25 U
203.00 USD
ALX-201-094-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P51878:
Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500:
Caspase-5 (mouse)
Further Categories Containing This Product:
Enzymes
•
Inflammasome
•
Recombinant Proteins/Fusion Proteins
ALX-201-106
Revised 08-Mar-07
Sp1 Protein (human) (recombinant) (HA)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Sp Protein Family/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-106-R050
50 µl
377.00 USD
Product Specification
SOURCE/HOST:
Produced in insect cells (Schneider SL2 cells). Human Sp1 is fused to a HA- and a FLAG
®
-tag (H. Braun and G. Suske; Biotechniques
26
, 1038 (1999)).
QUANTITY:
Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1µM ZnSO
4
, 37.5µg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay.
PURITY DETAIL:
DNA-affinity purified.
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.9, containing 1M KCl, 0.1% NP-40, 12.5mM MgCl
2
and 20% glycerol.
SPECIFIC ACTIVITY:
0.5BFU/µl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
APPLICATION:
EMSA promoter characterization,
in vitro
transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Description
The ubiquitously expressed Sp1 was the first identified and cloned member of the Sp-family of mammalian transcription factors. The 778 aa human protein contains an 81 aa DNA-binding domain consisting of three C
2
H
2
-type zinc fingers close to the C-terminus. Two glutamine-rich activation domains are present adjacent to serine/threonine stretches in the N-terminal part of the protein. Sp1 recognizes specifically G-rich elements such as the GC-box (GGGGCGGGG) and the GT/CACC-box (GGTGTGGGG) found in housekeeping as well as in many tissue-specific and viral genes. The protein may be employed in the following fields of research:
i) Promoter studies: Identification of Sp1 binding promoter elements by bandshift or DNase1
footprint assays.
ii) Promoter studies: Activation by Sp1 in
in vitro
transcription assays.
iii) Detection of Sp1 interacting proteins.
Product Specific Literature References
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain:
J.T. Kadanoga, et al.; Cell
51
, 1079 (1987)
Abstract
Vectors for inducible expression of dual epitope-tagged proteins in insect cells:
H. Braun & G. Suske; Biotechniques
26
, 1038 (1999)
Abstract
The Sp-family of transcription factors:
G. Suske; Gene
238
, 291 (1999), (Review)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Nuclear extract was prepared from HA/FLAG-Sp1 expressing SL2 cells and precleared by unspecific DNA-affinity precipitation (multimerized oligonucleotide with HNF-3 binding site). Sp1 was then precipitated with a specific DNA-affinity matrix (multimerized double-stranded GC-box oligonucleotide with the sequence 5´-AGCTTCCGTTGGGGCGGGGCTTCACGTCGA-3´). Bound protein was eluted with 50mM TRIS-HCl, pH 7.9, containing 1M KCl, 0.1% NP40, 20% glycerol, 12.5mM MgCl
2
.
In EMSAs, DNA-protein complexes are separated from free (unbound) oligonucleotides by native gel-electrophoresis. The formation of a specific DNA-protein complex results in a lower mobility of this complex compared to the mobility of the free DNA.
The specificity of the DNA-protein interaction can be analyzed by competition or supershift experiments. An excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, whereas unspecific or mutated oligonucleotides have no effect. The presence of a specific antibody leads to the formation of a very slow migrating antibody-protein-DNA complex.
FLAG is a registered trademark of Sigma-Aldrich Co.
General Literature References
For an extended bibliography
click here.
:
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-107
Revised 17-Nov-06
Sp3 Protein (human) (recombinant) (HA)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Sp Protein Family/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-107-R050
50 µl
377.00 USD
Product Specification
SOURCE/HOST:
Produced in insect cells (Schneider SL2 cells). Human Sp3 is fused to a HA- and a FLAG
®
-tag (H. Braun and G. Suske; Biotechniques
26
, 1038 (1999)).
QUANTITY:
Sufficient for performing at least 25 protein-DNA interaction experiments in 10 mM HEPES (pH 7.9), 0.1 mM EDTA, 8.5 % Glycerol, 170 mM KCl, 1 µM ZnSO
4
, 37.5 µg/ml Poly dIdC, 10 mM DTT, and 1 mg/ml BSA, analyzed by gel shift assay.
PURITY:
~50% (SDS-PAGE)
FORMULATION:
Liquid. In 20mM TRIS-Cl, pH 7.9, containing 150mM KCl, 0.2mM EDTA and 20% glycerol.
SPECIFICITY:
Binds specifically to an oligonucleotide with the sequence: GGG GCG GGG.
SPECIFIC ACTIVITY:
>0.5 BFU/µl (band forming units) 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
APPLICATION:
EMSA promoter characterization,
in vitro
transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
Product Specific Literature References
The Sp-family of transcription factors:
G. Suske; Gene
238
, 291 (1999), (Review)
Abstract
Vectors for inducible expression of dual epitope-tagged proteins in insect cells:
H. Braun & G. Suske; Biotechniques
26
, 1038 (1999)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
FLAG is a registered trademark of Sigma-Aldrich Co.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-156
Revised 08-Dec-04
Histone H1.0 (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-156-M001
1 mg
170.00 USD
Product Specification
MW:
~21kDa.
SOURCE/HOST:
Produced in
E. coli.
PURITY:
≥98% (SDS-PAGE, HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in 100% ethanol, methanol, DMSO or aqueous solutions.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-157
Revised 23-Feb-07
Histone H1.3 (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones/Related Products
Ordering Information
Product Numbers:
Format:
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ALX-201-157-M001
1 mg
170.00 USD
Product Specification
MW:
~22kDa.
SOURCE/HOST:
Produced in
E. coli.
PURITY:
≥95% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in 100% ethanol, methanol, DMSO or aqueous solutions.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P16402:
Histone H1.3 (human)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-159
Revised 08-Dec-04
Histone H1.5 (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-159-M001
1 mg
170.00 USD
Product Specification
MW:
~23kDa.
SOURCE/HOST:
Produced in
E. coli.
PURITY:
≥98% (SDS-PAGE, HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in 100% ethanol, methanol, DMSO or aqueous solutions.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-160
Revised 08-Dec-04
Histone H1.2 (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-160-M001
1 mg
170.00 USD
Product Specification
MW:
~21kDa.
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥98% (SDS-PAGE, HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in 100% ethanol, methanol, DMSO or aqueous solutions.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-170
Revised 25-Oct-06
PARP-3 (human) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-3 (human) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-3
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-201-170-C020
20 µg
368.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
0.38mg/ml
PURITY:
≥97%
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 0.25mM EDTA, 7mM β-mercaptoethanol, 0.25mM PMSF and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression
:
A. Augustin, et al.; J. Cell Sci.
116
, 1551 (2003)
Abstract
;
Full Text
Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function:
P. Chang et al.; Nature Cell Biology
7
, 1133 (2005)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins/Fusion Proteins
ALX-201-176
Revised 30-Sep-05
Histone γ-H2AX (human) (recombinant)
SYNONYMS
Histone H2AFX (human) (recombinant)
Histone H2A.X (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Histones/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-176-M001
1 mg
290.00 USD
ALX-201-176-M005
5 mg
1'160.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥98% (SDS-PAGE; HPLC)
FORMULATION:
Lyophilized.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-178
Revised 17-May-06
SET7/9 (human) (recombinant)
SYNONYMS
Histone H3-K4 Methyltransferase (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA & RNA Methyl & Alkyl Transferases/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-178-C100
100 µg
125.00 USD
Product Specification
MW:
~40.7kDa.
EC:
2.1.1.43
SOURCE/HOST:
Produced in
E. coli.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 0.2M sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet:
T. Kwon, et al.; EMBO J.
22
, 292 (2003)
Abstract
General Information
Histone methyltransferase that transfers methyl groups to Lys
4
of histone H3, in complex with
S
-adenosyl-L-methionine.
General Literature References
Structure and catalytic mechanism of the human histone methyltransferase SET7/9
:
B. Xiao, et al.; Nature
421
, 652 (2003)
Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
ALX-201-199
Revised 11-Jan-07
CEBP-α (270-358) (human) (recombinant) (His)
SYNONYMS
CCAAT/Enhancer Binding Protein α (270-358) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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Add To Cart
ALX-201-199-C100
100 µg
220.00 USD
