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Transcription Factors / Related Products
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ALX-201-094 Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-094-U025   25 U 203.00 USD Add To Cart
ALX-201-094-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P51878: Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500: Caspase-5 (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Inflammasome • Enzymes
 
 
ALX-850-217 Revised 24-Jan-05
Caspase-5 Colorimetric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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ALX-850-217-KI01   25 tests 203.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-850-218 Revised 24-Jan-05
Caspase-5 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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ALX-850-218-KI01   25 tests 189.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate WEHD-AFC.
General Information
WEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-201-199 Revised 11-Jan-07
CEBP-α (270-358) (human) (recombinant) (His)
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SYNONYMS CCAAT/Enhancer Binding Protein α (270-358) (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-201-199-C100   100 µg 220.00 USD Add To Cart
Product Specification
MW: ~14.5kDa.
SOURCE/HOST: Produced in E. coli. bZip region of CEBP-α (aa 270-358).
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-200 Revised 04-Mar-05
CEBP-γ (39-147) (human) (recombinant) (His)
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SYNONYMS CCAAT/Enhancer Binding Protein γ (39-147) (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-201-200-C100   100 µg 220.00 USD Add To Cart
Product Specification
MW: ~16.5kDa.
SOURCE/HOST: Produced in E. coli. DNA binding domain of CEBP-γ (aa 39-147).
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-350-332 Revised 03-Apr-08
Celastrol
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SYNONYMS Tripterine
3-Hydroxy-24-nor-2-oxo-1(10),3,5,7-friedelatetraen-29-oic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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ALX-350-332-M005   5 mg 65.00 USD Add To Cart
ALX-350-332-M025   25 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C29H38O4
MW: 450.6
CAS NUMBER: 34157-83-0
SOURCE/HOST: Isolated from the root of Tripterygium sp.
PURITY: ≥98% (HPLC)
APPEARANCE: Red cubic crystals.
SOLUBILITY: Soluble in 100% ethanol, DMSO or dimethyl formamide; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Cell permeable triterpenoid anti-inflammatory and immunosuppressive compound. Antioxidant. Suppresses LPS-induced cytokine release in macrophages and monocytes (IC50=40nM for IL-1β and IL-1α, 80nM for IL-6, 110nM for prostaglandin E2 and 210nM for IL-8 and TNF-α). Suppresses nitric oxide (NO) production (IC50=230nM) and LPS-induced NF-κB activation (IC50=270nM). Inhibits chymotrypsin-like activity of 20S proteasome (IC50=2.5µM). Inhibits lipid peroxidation induced by ADP and Fe2+ in rat liver mitochondria (IC50=7µM). Induces expression of a wider set of heat shock proteins. Inhibits topoisomerse II.
Product Specific Literature References
The triterpene celastrol as a very potent inhibitor of lipid peroxidation in mitochondria: H. Sassa, et al.; BBRC 172, 890 (1990) Abstract
Structural basis of potent antiperoxidation activity of the triterpene celastrol in mitochondria: effect of negative membrane surface charge on lipid peroxidation: H. Sassa, et al.; Free Radic. Biol. Med. 17, 201 (1994) Abstract
Novel cytokine release inhibitors. Part III: Truncated analogs of tripterine: W. He, et al.; Bioorg. Med. Chem. Lett. 8, 3659 (1998) Abstract
Celastrol, a potent antioxidant and anti-inflammatory drug, as a possible treatment for Alzheimer’s disease: A.C. Allison, et al.; Prog. Neuropsychopharmacol. Biol. Psychiatry 25, 1341 (2001) Abstract
Antiinflammatory constituents of Celastrus orbiculatus inhibit the NF-kappaB activation and NO production: H.Z. Jin, et al.; J. Nat. Prod. 65, 89 (2002) Abstract
Apoptosis induction in HL-60 cells and inhibition of topoisomerase II by triterpene celastrol: M. Nagase, et al.; Biosci. Biotechnol. Biochem. 67, 1883 (2003) Abstract; Full Text
Celastrol inhibits pro-inflammatory cytokine secretion in Crohn’s disease biopsies: G.F. Pinna, et al.; BBRC 322, 778 (2004) Abstract
Celastrols as inducers of the heat shock response and cytoprotection: S.D. Westerheide, et al.; J. Biol. Chem. 279, 56053 (2004) Abstract; Full Text
Celastrol protects against MPTP- and 3-nitropropionic acid-induced neurotoxicity: C. Cleren, et al.; J. Neurochem. 94, 995 (2005) Abstract
Celastrol blocks neuronal cell death and extends life in transgenic mouse model of amyotrophic lateral sclerosis: M. Kiaei, et al.; Neurodegener. Dis. 2, 246 (2005) Abstract
Celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice: H. Yang, et al.; Cancer Res. 66, 4758 (2006) Abstract; Full Text
Celastrol, a novel triterpene, potentiates TNF-induced apoptosis and suppresses invasion of tumor cells by inhibiting NF-kappaB-regulated gene products and TAK1-mediated NF-kappaB activation: G. Sethi, et al.; Blood 109, 2727 (2007) Abstract
Induction of heat shock proteins in differentiated human and rodent neurons by celastrol: A.M. Chow & I.R. Brown; Cell Stress Chaperones 12, 237 (2007) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • Natural Products - Nitric Oxide Pathway Modulators • Stress & Heat Shock Proteins Other Products • Natural Products - Antioxidants • Natural Products - Immunomodulators • Natural Products - Topoisomerase Inhibitors • Ubiquitin & Proteasome Other Products
 
 
ALX-201-279 Revised 18-Mar-08
CREB Binding Protein (mouse) (recombinant) (His)
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SYNONYMS CBP (mouse) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY CREB & CREB Binding Protein / Related Products
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ALX-201-279-C002   2 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.3.1.48
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~50-100ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P45481: CREB Binding Protein (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Histone Acetyl- & Methyl-Transferases / Related Products
 
 
ALX-400-003 Revised 25-May-07
Diethyldithiocarbamic acid . sodium salt . trihydrate (high purity)
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SYNONYMS DETC . 3H2O (high purity)
Diethyldithiocarbamate . 3H2O (high purity)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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ALX-400-003-G005   5 g 17.00 USD Add To Cart
ALX-400-003-G025   25 g 27.00 USD Add To Cart
Product Specification
FORMULA: C5H10NS2 . Na . 3H2O
MW: 148.3 . 23.0 . 54.0
CAS NUMBER: 20624-25-3
MERCK INDEX: 14: 3378
RTECS: EZ6550000
PURITY: ≥99%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: HARMFUL.

Product Description
Nitric oxide (NO) spin-trapping reagent. Thiol and iron chelator. Inhibits induction of macrophage nitric oxide synthase (NOS). Has been shown to be an inhibitor of the nuclear transcription factor κB (NF-κB).
Product Specific Literature References
On-line detection of nitric oxide formation in liquid aqueous phase by electron paramagnetic resonance spectroscopy: P. Mordvintcev, et al.; Anal. Biochem. 199, 142 (1991) Abstract
NO accounts completely for the oxygenated nitrogen species generated by enzymic L-arginine oxygenation: A. Mülsch, et al.; Biochem. J. 288, 597 (1992) Abstract
Iron diethyldithiocarbamate as spin trap for nitric oxide detection: A.F. Vanin; Methods Enzymol. 301, 269 (1999), Review Abstract
Diethyldithiocarbamate inhibits induction of macrophage NO synthase: A. Mülsch, et al.; FEBS Lett. 321, 215 (1993) Abstract
The relationship between L-arginine-dependent nitric oxide synthesis, nitrite release and dinitrosyl-iron complex formation by activated macrophages: A. Vanin, et al.; Biochim. Biophys. Acta 1177, 37 (1993) Abstract
Dithiocarbamates as potent inhibitors of nuclear factor kappa B activation in intact cells: R. Schreck, et al.; J. Exp. Med. 175, 1181 (1992) Abstract
In vivo spin trapping of nitric oxide generated in the small intestine, liver, and kidney during the development of endotoxemia: a time-course study: G. Wallis, et al.; Shock 6, 274 (1996) Abstract
Epr evidence of nitric oxide production by the regenerating rat liver: M. Obolenskaya, et al.; BBRC 202, 571 (1994) Abstract
EPR and laser flash photolysis studies of the reaction of nitric oxide with water soluble NO trap Fe(II)-proline-dithiocarbamate complex: S.V. Paschenko, et al.; BBRC 225, 577 (1996) Abstract
Complexes of Fe2+ with diethyldithiocarbamate or N-methyl-D-glucamine dithiocarbamate as traps of nitric oxide in animal tissues: comparative investigations: V.D. Mikoyan, et al.; Biochim. Biophys. Acta 1336, 225 (1997) Abstract
NO-dependent mechanisms of adaptation to hypoxia: I.Y. Malyshev, et al.; Nitric Oxide 3, 105 (1999) Abstract
Ex vivo EPR detection of nitric oxide in brain tissue: H. Fujii & L.J. Berliner; Magn. Reson. Med. 42, 599 (1999) Abstract
EPR spectroscopy of common nitric oxide - spin trap complexes: S. Nedeianu & T. Pali; Cell Mol. Biol. Lett. 7, 142 (2002) Abstract
EPR quantification of vascular nitric oxide production in genetically modified mouse models: J.P. Khoo, et al.; Nitric Oxide 10, 156 (2004) Abstract
Evaluation of lipid-based carrier systems and inclusion complexes of diethyldithiocarbamate-iron to trap nitric oxide in biological systems: N. Charlier, et al.; Magn. Reson. Med. 55, 215 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • NOS Inhibitors (NOS Induction & Enzyme Activity)
 
 
ALX-280-017 Revised 31-Oct-06
Diethylmaleate
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SYNONYMS 2-Butenedioic acid
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-280-017-G005   5 g 15.00 USD Add To Cart
Product Specification
FORMULA: C8H12O4
MW: 172.2
CAS NUMBER: 141-05-9
MERCK INDEX: 14: 3123
RTECS: ON1225000
PURITY: ≥97%
APPEARANCE: Clear liquid.
SOLUBILITY: Soluble in 100% ethanol or ether; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: MAY BE MUTAGENIC. IRRITANT. MAY BE CARCINOGENIC.

Product Description
Reduces NF-κB activation through the formation of conjugates with NF-κB.
Product Specific Literature References
Acrolein causes inhibitor kappaB-independent decreases in nuclear factor kappaB activation in human lung adenocarcinoma (A549) cells: N.D. Horton, et al.; J. Biol. Chem. 274, 9200 (1999) Abstract; Full Text
 
 
ALX-201-074 Revised 18-Sep-07
EGR-1 Protein (human) (recombinant)
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SYNONYMS Early Growth Response Protein 1 (human) (recombinant)
NGFI-A (human) (recombinant)
krox24 (human) (recombinant)
TIS-8 (human) (recombinant)
zif 268 (human) (recombinant)
pat 225 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Egr Protein Family / Related Products
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ALX-201-074-1   1 Vial 365.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 50µl, corresponding to >25 band forming units (BFU). Sufficient for performing at least 25 gel shift assays under standard conditions [2].
FORMULATION: Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
SPECIFICITY: Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
SPECIFIC ACTIVITY: >0.5BFU/µl. 1BFU is sufficient to generate a band shift with a labelled oligonucleotide.
APPLICATION: EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
The recombinant EGR-1 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-1 binding promoter elements. b) Detection of EGR-1 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-1. d) Screening for EGR-1 activity in tumor cells and lines. e) Drug screening: Effect on EGR-1 generation and DNA binding.
Product Specific Literature References
A regulatory element in the human interleukin 2 gene promoter is a binding site for the zinc finger proteins Sp1 and EGR-1: C. Skerka, et al.; J. Biol. Chem. 270, 22500 (1995) Abstract; Full Text
The early growth response protein (EGR-1) regulates interleukin-2 transcription by synergistic interaction with the nuclear factor of activated T cells: E.L. Decker, et al.; J. Biol. Chem. 273, 26923 (1998) Abstract; Full Text
The human zinc finger protein EGR-4 acts as autoregulatory transcriptional repressor: P.F. Zipfel, et al.; Biochim. Biophys. Acta. 1354, 134 (1997) Abstract
Zinc finger transcription factors as molecular targets for nitric oxide-mediated immunosuppression: inhibition of IL-2 gene expression in murine lymphocytes: D. Berendji, et al.; Mol. Med. 5, 721 (1999) Abstract
Fibroblast growth factor-2 induction of platelet-derived growth factor-C chain transcription in vascular smooth muscle cells is ERK-dependent but not JNK-dependent and mediated by Egr-1: V.C. Midgley and L.M. Khachigian; J. Biol. Chem. 279, 40289 (2004) Abstract; Full Text
General Information
Early Growth Response- (EGR-) proteins represent a family of related transcription factors with almost identical DNA- binding zinc finger domains, but with different flanking regions. All four human EGR-proteins, EGR-1 to EGR-4 bind the EGR-consensus sequence GCG T/GGG GCG. However individual EGR-proteins show different binding to related sequences, such as the overlapping EGR-1/Sp1 binding site in the human IL-2 gene promoter. In functional assays the EGR-4 protein shows superior performance for Electrophoretic Mobility Shift Assay (EMSA) compared to proteins expressed in E.coli.
BACKGROUND/TECHNICAL INFORMATION For EMSA labeled oligonucleotides are required, that include the specific target sequence. Radioactive labeling of the oligonucleotide is performed by kinase reaction (T4 kinase) using either 32P-γ ATP or 33P-γ ATP as a source for radioactivity. Due to the lower radiation load we recommend the use of 33P-γ ATP, which results in specific, detectable bands. Formation of a specific protein: DNA complex results in a lower mobility of this complex compared to the mobility of the free DNA. The specificity of the EMSA can also be analyzed by competition experiments in which an excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, while the same amount of unrelated or mutated oligonucleotide has no effect. DNA-Protein complexes are then separated from the free (unbound) oligonucleotides by native gel-electrophoresis.

Swiss-Prot link P18146: EGR-1 (human)
AfCS Signa