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DNA Metabolism
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ALX-201-233 Revised 22-Jun-07
Rad9/Rad1/Hus1 (human) (recombinant)
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SYNONYMS 9-1-1 Complex (human) (recombinant)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY DNA Damage Checkpoint Other Products
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ALX-201-233-C005   5 µg 240.00 USD Add To Cart
Product Specification
MW: 106kDa (heterotrimer of 43, 31 and 32kDa)
SOURCE/HOST: Produced in baculovirus.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (by SDS-PAGE).
FORMULATION: Liquid. In 25mM Tris, pH 8.0, containing 20% glycerol, 100mM sodium chloride and 1mM β-mercaptoethanol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair: M. Toueille, et al.; Nucl. Acids Res. 32, 3316 (2004) Abstract; Full Text
The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery: E. Smirnova, et al.; Biochem. J. 389, 13 (2005) Abstract
General Literature References
Regulation of the DNA replication fork: a way to fight genomic instability: M. Toueille and U. Hubscher; Chromosoma 113, 113 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Replication Other Products
 
 
ALX-201-234 Revised 07-Nov-07
Proliferating Cell Nuclear Antigen (human) (recombinant)
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SYNONYMS PCNA (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Proliferating Cell Nuclear Antigen [PCNA] / Related Products
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ALX-201-234-C025   25 µg 240.00 USD Add To Cart
Product Specification
MW: 87kDa (homotrimer of 29kDa)
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 50mM sodium chloride, 1mM DTT and 1mM EDTA.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 4 weeks when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The solution structure of functionally active human proliferating cell nuclear antigen determined by small-angle neutron scattering: P. Schurtenberger, et al.; J. Mol. Biol. 275, 123 (1998) Abstract
General Literature References
Proliferating cell nuclear antigen (PCNA): a dancer with many partners: G. Maga and U. Hubscher; J. Cell Sci. 116, 3051 (2003) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Tumor Markers
 
 
ALX-201-278 Revised 30-Sep-08
DNA Ligase I (human) (recombinant) (His)
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SYNONYMS DNA Ligase 1 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Replication Other Products
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ALX-201-278-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~102kDa. Note: The mobility of DNA ligase I in a PAA gel is not corresponding to its MW; it runs at ~125kDa.
SOURCE/HOST: Produced in E. coli. Full length human DNA ligase I is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.6mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM HEPES-KOH, pH 7.5, containing 30mM sodium chloride, 1mM EDTA, 1mM DTT and 30% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
USE/STABILITY: Enzyme is freshly produced upon customer request and is stable for ~2 weeks at -80°C. Very prone to degradation even in the absence of proteases. Freeze in liquid nitrogen for long term storage.
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair: A. Gembka, et al.; Nucleic Acids Res. 35, 2596 (2007) Abstract; Full Text
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Repair Other Products
 
 
ALX-201-296 Revised 15-Oct-08 New product
Replication Protein A (human) (recombinant)
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SYNONYMS RP-A (human) (recombinant)
Replication Factor A (human) (recombinant)
RF-A (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-296-C005   5 µg 260.00 USD Add To Cart
Product Specification
MW: ~118kDa (heterotrimer with subunits of 70, 32/34 and 14kDa)
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.16mg/ml
PURITY: ≥98% (SDS-PAGE).
FORMULATION: Liquid. In 20mM TRIS, pH 8.0, containing 10% (v/v) glycerol, 2mM EDTA and 1mM DTT.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for more then 2 year when stored at  -80°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Recombinant replication protein A: expression, complex formation, and functional characterization: L.A. Henricksen, et al.; J. Biol. Chem. 269, 11121 (1994) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot links
P27694: RFA1 (human)
P15927: RFA2 (human)
P35244: RFA3 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Replication Other Products
 
 
ALX-210-763 Revised 14-Dec-05
Polyclonal Antibody to Topoisomerase IIα (human)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Topoisomerase Antibodies
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ALX-210-763-C500   500 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified IgG.
FORMULATION: Liquid. In 10mM PBS, pH 7.4, containing 0.2% BSA and 0.09% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1513-1530 (R1513AKKPIKYLEESDEDDLF1530) of C-terminal human topoisomerase IIα.
SPECIFICITY: Recognizes human topoisomerase IIα. Detects a band of ~170kDa by Western blot. Does not cross-react with topoisomerase IIβ or I.
APPLICATION: Immunocytochemistry
Immunohistochemistry (frozen sections,  paraffin sections)
Immunoprecipitation (use Protein A; 10µg/mg protein lysate)
Western Blot (excellent) (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Topoisomerase IIα plays important roles in the synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase IIα is also implicated in drug resistance of tumor cells. Note: Ki67 is also present in G1-phase.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-270-095 Revised 07-May-08
5,6-Dichloro-1-β-D-ribofuranosyl benzimidazole
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SYNONYMS DRB
5,6-Dichlorobenzimidazole riboside
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Adenosine Derivatives Other Products
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ALX-270-095-M005   5 mg 20.00 USD Add To Cart
ALX-270-095-M025   25 mg 70.00 USD Add To Cart
Product Specification
FORMULA: C12H12Cl2N2O4
MW: 319.1
CAS NUMBER: 53-85-0
RTECS: DD7310000
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in 100% ethanol, DMSO (100mM) or dimethyl formamide (100mM).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
USE/STABILITY: Solutions should be stored in the refrigerator and should be lyophilized and frozen for longer storage periods.
IDENTITY: Identity determined by 1H-NMR, MS and UV.

Product Description
Selective casein kinase II inhibitor. Inhibitor of RNA synthesis in eukaryotic cells.
Product Specific Literature References
Halobenzimidazole ribosides and RNA synthesis of cells and viruses: I. Tamm & P. Sehgal; Adv. Virus Res. 22, 187 (1978) Abstract
Mechanism of action of 5,6-dichloro-1-beta-D- ribofuranosylbenzimidazole. II. A resistant human cell mutant with an altered transcriptional machinery: B. Mittleman, et al.; J. Mol. Biol. 165, 461 (1983) Abstract
Mechanism of action of DRB. III. Effect on specific in vitro initiation of transcription: R. Zandomeni, et al.; J. Mol. Biol. 167, 561 (1983) Abstract
Casein kinase type II is involved in the inhibition by 5,6-dichloro-1- beta-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription: R. Zandomeni, et al.; J. Biol. Chem. 261, 3414 (1986) Abstract; Full Text
Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6- dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB): C.E. Farin & L. Yang; Mol. Reprod. Dev. 37, 284 (1994) Abstract
Further Categories Containing This Product:
Casein Kinase Inhibitors
 
 
ALX-270-201 Revised 18-Sep-07
Aurintricarboxylic acid
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SYNONYMS ATA
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Topoisomerase Inhibitors Other Products
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ALX-270-201-G001   1 g 15.00 USD Add To Cart
Product Specification
FORMULA: C22H14O9
MW: 422.4
CAS NUMBER: 4431-00-9
PURITY: ~85%
APPEARANCE: Red solid.
SOLUBILITY: Soluble in aqueous alkali.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.

Product Description
Potent inhibitor of DNA topoisomerase II in vitro and protein-nucleic acid interactions. Stimulates the tyrosine phosphorylation of MAP kinases. Inhibits both major calpain isoforms. Potent and selective inhibitor of SARS coronavirus replication. Apoptosis inhibitor.
Product Specific Literature References
Mechanism of action of polymeric aurintricarboxylic acid, a potent inhibitor of protein--nucleic acid interactions: R.G. Gonzalez, et al.; Biochemistry 19, 4299 (1980) Abstract
Delayed application of aurintricarboxylic acid reduces glutamate- induced cortical neuronal injury: C.A. Csernansky, et al.; J. Neurosci. Res. 38, 101 (1994) Abstract
Inhibition of topoisomerase II by aurintricarboxylic acid: implications for mechanisms of apoptosis: D.R. Catchpoole & B.W. Stewart; Anticancer Res. 14, 853 (1994) Abstract
Aurintricarboxylic acid, a putative inhibitor of apoptosis, is a potent inhibitor of DNA topoisomerase II in vitro and in Chinese hamster fibrosarcoma cells: Y. Benchokroun, et al.; Biochem. Pharmacol. 49, 305 (1995) Abstract
A neuroprotective compound, aurin tricarboxylic acid, stimulates the tyrosine phosphorylation cascade in PC12 cells: N. Okada & S. Koizumi; J. Biol. Chem. 270, 16464 (1995) Abstract; Full Text
Potent and selective inhibition of SARS coronavirus replication by aurintricarboxylic acid: R. He, et al.; BBRC 320, 1199 (2004) Abstract
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
 
 
ALX-270-209 Revised 05-Apr-08
Etoposide
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SYNONYMS 4-Desmethylepipodophyllotoxin 9-(4,6-O-ethylidene)-β-D-glucopyranoside
VP-16-213
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Topoisomerase Inhibitors
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ALX-270-209-M025   25 mg 30.00 USD Add To Cart
ALX-270-209-M100   100 mg 65.00 USD Add To Cart
ALX-270-209-M500   500 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C29H32O13
MW: 588.6
CAS NUMBER: 33419-42-0
MERCK INDEX: 14: 3886
RTECS: KC0190000
SOURCE/HOST: Semisynthetic derivative of podophyllotoxin.
PURITY: ≥99%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, ethyl ether or chloroform:methanol (1:1); slightly soluble in methanol or acetone; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
USE/STABILITY: Store solutions in DMSO at 4°C. For long term storage prepare aliquots and store at -20°C.
HANDLING: Protect from light and moisture.
HAZARD: MAY BE CARCINOGENIC. TOXIC.

Product Description
Antitumor reagent. Topoisomerase II inhibitor. Induces apoptosis by FasL. Activates p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cancer cells.
Product Specific Literature References
The podophyllotoxin derivatives VP16-213 and VM26: B.F. Issell; Cancer Chemother. Pharmacol. 7, 73 (1982) Abstract
Etoposide: four decades of development of a topoisomerase II inhibitor: K.R. Hande; Eur. J. Cancer 34, 1514 (1998) Abstract
Etoposide sensitivity of human prostatic cancer cell lines PC-3, DU 145 and LNCaP: M. Salido, et al.; Histol. Histopathol. 14, 125 (1999) Abstract
A systematic review of the role of etoposide and cisplatin in the chemotherapy of small cell lung cancer with methodology assessment and meta-analysis: C. Mascaux, et al.; Lung Cancer 30, 23 (2000) Abstract
Distinct pathways for stimulation of cytochrome c release by etoposide: J.D. Robertson, et al.; J. Biol. Chem. 275, 32438 (2000) Abstract; Full Text
Ordering of ceramide formation, caspase activation, and Bax/Bcl-2 expression during etoposide-induced apoptosis in C6 glioma cells: M. Sawada, et al.; Cell Death Differ. 7, 761 (2000) Abstract
Etoposide-induced apoptosis is not associated with the fas pathway in acute myeloblastic leukemia cells: T. Siitonen, et al.; Leuk. Res. 24, 281 (2000) Abstract
Early caspase activation in leukemic cells subject to etoposide-induced G2-M arrest: evidence of commitment to apoptosis rather than mitotic cell death: R.J. Sleiman & B.W. Stewart; Clin. Cancer Res. 6, 3756 (2000) Abstract; Full Text
Cell cycle phase specificity in the potentiation of etoposide-induced DNA damage and apoptosis by KN-62, an inhibitor of calcium-calmodulin- dependent enzymes: M. Aoyama, et al.; Biochem Pharmacol 61, 49 (2001) Abstract
Deacetylase activity associates with topoisomerase II and is necessary for etoposide-induced apoptosis: C.A. Johnson, et al.; J. Biol. Chem. 276, 4539 (2001) Abstract; Full Text
In vitro topo II-DNA complex accumulation and cytotoxicity of etoposide in leukaemic cells from patients with acute myelogenous and chronic lymphocytic leukaemia: Y. Wang, et al.; Leuk. Res. 25, 133 (2001) Abstract
Etoposide: discovery and medicinal chemistry: P. Meresse, et al.; Curr. Med. Chem. 11, 2443 (2004), Review Abstract
Etoposide, topoisomerase II and cancer: E.L. Baldwin & N. Osheroff; Curr. Med. Chem. Anticancer Agents 5, 363 (2005), Review Abstract
Further Categories Containing This Product:
Other Toxins • Antitumor Agents (Enzyme Inhibitors) • Natural Products - Antitumor Reagents • Natural Products - Apoptosis Inducers & Inhibitors • Antitumor Agents (Apoptosis Inducers)
 
 
ALX-270-262 Revised 13-Feb-08
Ellagic acid . dihydrate
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SYNONYMS 4,4',5,5',6,6'-Hexahydroxydiphenic acid 2,6,2',6'-dilactone . 2H2O
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antioxidants
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ALX-270-262-M100   100 mg 12.00 USD Add To Cart
Product Specification
FORMULA: C14H6O8 . 2H2O
MW: 302.2 . 36.0
CAS NUMBER: 476-66-4
MERCK INDEX: 14: 3547
RTECS: DJ2620000
SOURCE/HOST: Isolated from chestnut bark.
PURITY: ≥97%
APPEARANCE: Grey to green solid.
SOLUBILITY: Soluble in 1M sodium hydroxide (10mg/ml) or pyridine.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep under inert gas. Protect from light.
HAZARD: IRRITANT.
IDENTITY: Determined by MS and NMR.

Product Description
Polyphenol antioxidant with antitumor properties. Inhibitor of topoisomerases. Inhibits VEGF-induced phosphorylation of VEGFR-2 and PDGF-induced phosphorylation of PDGFR. Induces apoptosis.
Product Specific Literature References
The dietary anticancer agent ellagic acid is a potent inhibitor of DNA topoisomerases in vitro: A. Constantinou, et al.; Nutr. Cancer 23, 121 (1995) Abstract
Novel inhibitors of 3-phosphoglycerate kinase: M.J. Hickey, et al.; Biochem. Soc. Trans. 23, 607S (1995) Abstract
Inhibitory actions of ellagic acid on growth and arylamide N-acetyltransferase activity in strains of Heliobacter pylori from peptic ulcer patients: J.G. Chung; Microbios 93, 115 (1998) Abstract
Polyphenols inhibit promotional phase of tumorigenesis: relevance of superoxide radicals: A. Kaul & K.L. Khanduja; Nutr. Cancer 32, 81 (1998) Abstract
Inhibition of eukaryote serine/threonine-specific protein kinases by piceatannol: B.H. Wang, et al.; Planta Med. 64, 195 (1998) Abstract
p53/p21 (WAF1CIP1) expression and its possible role in G1 arrest and apoptosis in ellagic acid treated cancer cells: B.A. Narayanan, et al.; Cancer Lett. 136, 215 (1999) Abstract
Combined inhibition of PDGF and VEGF receptors by ellagic acid, a dietary-derived phenolic compound: L. Labrecque, et al.; Carcinogenesis 26, 821 (2005) Abstract
Antioxidant and apoptosis-inducing activities of ellagic acid: D.H. Han, et al.; Anticancer Res. 26, 3601 (2006) Abstract
The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells: C.H. Chen, et al.; Mol. Nutr. Food Res. 51, 962 (2007) Abstract
Ellagic acid, a natural polyphenol protects rat peripheral blood lymphocytes against nicotine-induced cellular and DNA damage in vitro: with the comparison of N-acetylcysteine: A.R. Sudheer, et al.; Toxicology 230, 11 (2007) Abstract
Further Categories Containing This Product:
Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Antitumor Agents (Enzyme Activators & Inducers) • Natural Products - Topoisomerase Inhibitors • Antitumor Agents (Enzyme Inhibitors) • Natural Products - Antitumor Reagents • Polyphenols Other Products • Natural Products - Apoptosis Inducers & Inhibitors
 
 
ALX-270-286 Revised 18-Sep-07
C75
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SYNONYMS trans-4-Carboxy-5-octyl-3-methylene-butyrolactone
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Fatty Acid Synthase [FAS] / Related Products
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