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Aryl Hydrocarbon Receptor / Related Products
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Items 8 of 8
ALX-804-108 Revised 18-Sep-07
Monoclonal Antibody to Ah Receptor Nuclear Translocator (2B10)
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SYNONYMS anti-ARNT MAb (2B10)
anti-HIF-1β MAb (2B10)
anti-Hypoxia-inducible Factor 1β MAb (2B10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-108-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 2B10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 771-789 (N771SYNNEEFPDLTMFPPFSE789) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY: Recognizes human, mouse and monkey ARNT. Detects a band of ~87kDa by Western blot.
APPLICATION: Immunocytochemistry (1:1'000)
Immunoprecipitation
Western Blot (1:4'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor translocator: Characterization of two monoclonal antibodies to the ARNT: N.G. Hord & G. Perdew; Mol. Pharmacol. 46, 618 (1994) Abstract
Oxygen tension modulates beta-globin switching in embryoid bodies: S. Bichet, et al.; FASEB J. 13, 285 (1999) Abstract; Full Text
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell Sci. 112, 1203 (1999) Abstract; Full Text
Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein-mediated transactivation potential in a dimer context: W.P. Long, et al.; J. Biol. Chem. 274, 12391 (1999) Abstract; Full Text
DNA methylation represses the expression of the human erythropoietin gene by two different mechanisms: H. Yin and K.L. Blanchard; Blood 95, 111 (2000) Abstract; Full Text
A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses: Y.S. Chun, et al.; J. Cell Sci. 114, 4051 (2001) Abstract; Full Text
Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression: T. Sanchez-Elsner, et al.; J. Biol. Chem. 276, 38527 (2001) Abstract; Full Text
Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia: H. Suzuki, et al.; Oncogene 20, 5779 (2001) Abstract; Full Text
Rescue of hypoxia-inducible factor-1alpha-deficient tumor growth by wild-type cells is independent of vascular endothelial growth factor: G. Höpfl, et al.; Cancer Res. 62, 2962 (2002) Abstract; Full Text
The arylhydrocarbon receptor (AhR), but not the AhR-nuclear translocator (ARNT), is increased in hearts of patients with cardiomyopathy: M.R. Mehrabi, et al.; Virchows Arch. 441, 481 (2002) Abstract; Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products • Monoclonal Antibodies
 
 
ALX-804-421 Revised 26-Nov-04
Monoclonal Antibody to Aryl Hydrocarbon Receptor (RPT1)
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SYNONYMS anti-AhR MAb (RPT1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-421-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: RPT1
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 12-17 (R12KRRKP17) and aa 22-31 (V22KPIPAEGIK31) of mouse AhR (aryl hydrocarbon receptor).
SPECIFICITY: Recognizes human, mouse and rat AhR. Detects a band of ~95kDa by Western blot.
APPLICATION: Immunohistochemistry (paraffin sections)
Western Blot (1:1'000)
For immunoprecipitation, use MAb to AhR (RPT9) (Prod. No. ALX-804-423).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex: H.S. Chen & G.H. Perdew; J. Biol. Chem. 269, 27554 (1994) Abstract; Full Text
General Information
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-423 Revised 12-Jun-08
Monoclonal Antibody to Aryl Hydrocarbon Receptor (RPT9)
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SYNONYMS anti-AhR MAb (RPT9)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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ALX-804-423-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: RPT9
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 12-17 (R12KRRKP17) and aa 22-31 (V22KPIPAEGIK31) of mouse AhR (aryl hydrocarbon receptor).
SPECIFICITY: Recognizes human, mouse and rat AhR. Detects a band of ~95kDa by Western blot.
APPLICATION:

Gel Shift Assay
Immunocytochemistry
Immunohistochemistry (paraffin sections (1:100); frozen sections)
Immunoprecipitation, Chromatin Immunoprecipitation (1:100)
Note: For Western blot use MAb to AhR (RPT1) (Prod. No. ALX-804-421).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-216 Revised 24-Oct-08
Monoclonal Antibody to HIF-1α (mgc3)
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SYNONYMS anti-Hypoxia-inducible Factor 1α MAb (mgc3)
anti-ARNT-interacting Protein MAb (mgc3)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Hypoxia-inducible Factor [HIF] / Related Products
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ALX-804-216-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
Monkey
CLONE: mgc3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).
SPECIFICITY: Recognizes human, mouse, monkey, bovine and pig HIF-1α. Detects a band of ~116kDa by Western blot after hypoxic induction. Does not cross-react with HIF-1β or HIF-2α.
APPLICATION: Gel Supershift Assay (mouse HIF-1α only)
Immunocytochemistry (1:200)
Immunoprecipitation
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
[1] General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobuilins raised against thy hypoxia-inducible factor 1alpha: G. Camenisch, et al.; FASEB J. 13, 81 (1999) Abstract; Full Text
[2] Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell. Sci. 112, 1203 (1999) Abstract; Full Text
General Information
Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.
Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products • Monoclonal Antibodies
 
 
ALX-210-245 Revised 21-Nov-06
Polyclonal Antibody to Ah Receptor Nuclear Translocator 3
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SYNONYMS anti-ARNT3 PAb
anti-BMAL1 PAb
anti-Brain & Muscle ARNT-like 1 PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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ALX-210-245-R200   200 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 582-594 (CD582MIDNDQG
SSSPS594) of mouse ARNT3 (Ah receptor nuclear translocator 3).
SPECIFICITY: Recognizes human and hamster ARNT3.
APPLICATION: Western Blot (1:200)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-152-023.
General Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK-BMAL1 has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian the timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Circadian Transcriptional Regulators / Related Products • Polyclonal Antibodies
 
 
ALX-210-150 Revised 15-Oct-07
Polyclonal Antibody to Ah Receptor Nuclear Translocator (mouse)
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SYNONYMS anti-ARNT (mouse) PAb
anti-HIF-1β (mouse) PAb
anti-Hypoxia-inducible Factor 1β (mouse) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-150-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 771-789 (N771SYNNEEFPDLTMFPPFSE789) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY: Recognizes mouse ARNT.
APPLICATION: Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Literature References
Cloning of a factor required for activity of the Ah (Dioxin) receptor: E. Hoffman, et al.; Science 252, 954 (1991) Abstract
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocation: characterization of two monoclonal antibodies to the ARNT   : N.G. Hord and G. Perdew; Mol. Pharmacol. 46, 618 (1994) Abstract
cDNA cloning and tissue-specific expression of a novel basic helix/loop/helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear transporter: K. Hirose, et al.; Mol. Cell. Biol. 16, 1706 (196) Abstract
Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts: C. Vaziri, et al.; J. Biol. Chem. 271, 25921 (1996) Abstract; Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products • Polyclonal Antibodies
 
 
ALX-210-069 Revised 16-Jul-08
Polyclonal Antibody to HIF-1α
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SYNONYMS anti-Hypoxia-inducible Factor 1α PAb
anti-ARNT-interacting Protein PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-069-C050   50 µg 220.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 2.8mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant HIF-1α (hypoxia-inducible factor-1a) (aa 609-826).
SPECIFICITY: Recognizes human and mouse HIF-1α.
APPLICATION: Immunohistochemistry (1:200)
Immunoprecipitation (1-5µg)
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Loss of HIF-1alpha in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis: N. Tang, et al.; Cancer Cell 6, 485 (2004) Abstract
Epidermal sensing of oxygen is essential for systemic hypoxic response: A.T. Boutin, et al.; Cell 133, 206 (2008) Abstract
Critical Role of HIF-1alpha in Keratinocyte Defense against Bacterial Infection: C. Peyssonnaux, et al.; J. Invest. Dermatol. 128, 1964 (2008) Abstract
General Literature References
Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin: C. C. Hudson, et al.; Mol. Cell Biol. 22, 7004 (2002) Abstract; Full Text
Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products • Polyclonal Antibodies
 
 
ALX-215-058 Revised 12-Sep-07
Polyclonal Antibody to HIF-2α (human)
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SYNONYMS anti-Hypoxia-inducible Factor 2α (human) PAb
anti-EPAS-1 (human) PAb
anti-Endothelial PAS Domain-containing Protein 1 (human) PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
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ALX-215-058-R200   200 µl 267.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 426-443 of human HIF-2α (hypoxia-inducible factor 2α).
SPECIFICITY: Recognizes human HIF-2α. Detects a band of ~90kDa.
APPLICATION: Western Blot (1:500-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at -4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products • Polyclonal Antibodies
 
 
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