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DNA Metabolism
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ALX-210-763 Revised 14-Dec-05
Polyclonal Antibody to Topoisomerase IIα (human)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Topoisomerase Antibodies
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ALX-210-763-C500   500 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified IgG.
FORMULATION: Liquid. In 10mM PBS, pH 7.4, containing 0.2% BSA and 0.09% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1513-1530 (R1513AKKPIKYLEESDEDDLF1530) of C-terminal human topoisomerase IIα.
SPECIFICITY: Recognizes human topoisomerase IIα. Detects a band of ~170kDa by Western blot. Does not cross-react with topoisomerase IIβ or I.
APPLICATION: Immunocytochemistry
Immunohistochemistry (frozen sections,  paraffin sections)
Immunoprecipitation (use Protein A; 10µg/mg protein lysate)
Western Blot (excellent) (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Topoisomerase IIα plays important roles in the synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase IIα is also implicated in drug resistance of tumor cells. Note: Ki67 is also present in G1-phase.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-201-234 Revised 07-Nov-07
Proliferating Cell Nuclear Antigen (human) (recombinant)
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SYNONYMS PCNA (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Proliferating Cell Nuclear Antigen [PCNA] / Related Products
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ALX-201-234-C025   25 µg 240.00 USD Add To Cart
Product Specification
MW: 87kDa (homotrimer of 29kDa)
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 50mM sodium chloride, 1mM DTT and 1mM EDTA.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 4 weeks when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The solution structure of functionally active human proliferating cell nuclear antigen determined by small-angle neutron scattering: P. Schurtenberger, et al.; J. Mol. Biol. 275, 123 (1998) Abstract
General Literature References
Proliferating cell nuclear antigen (PCNA): a dancer with many partners: G. Maga and U. Hubscher; J. Cell Sci. 116, 3051 (2003) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Tumor Markers
 
 
ALX-400-043 Revised 09-May-07
Propidium Iodide
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-400-043-M001   1 mg 77.00 USD Add To Cart
Product Specification
FORMULA: C27H34N4I2
MW: 668.4
CAS NUMBER: 25535-16-4
RTECS: SF7949600
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Solution in PBS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.

Product Description
DNA intercalator. Useful for differentiation between apoptotic cell death if used in combination with annexin V conjugates by flow cytometry. Ex(max): 536nm; Em(max): 617nm.
Product Specific Literature References
Propidium iodide staining correlates with the extent of DNA degradation in isolated nuclei: T. Crompton, et al.; BBRC 183, 532 (1992) Abstract
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells: F. Belloc, et al.; Cytometry 17, 59 (1994) Abstract
Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide: O. Honda, et al.; Int. J. Oncol. 16, 283 (2000) Abstract
Flow cytometric analysis of apoptotic subpopulations with a combination of annexin V-FITC, propidium iodide, and SYTO 17: M. Eray, et al.; Cytometry 43, 134 (2001) Abstract
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
Distribution of the human intracellular serpin protease inhibitor 8 in human tissues: M.C. Strik, et al.; J. Histochem. Cytochem. 50, 1443 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Dilute to 250µg/ml in PBS before use. Add 1µl per annexin V assay.
Related Products
Further Categories Containing This Product:
DNA Intercalators & Crosslinkers
 
 
ALX-350-285 Revised 19-Feb-08
Proscillaridin A
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - ATPase Inhibitors
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ALX-350-285-M005   5 mg 17.00 USD Add To Cart
Product Specification
FORMULA: C30H42O8
MW: 530.7
CAS NUMBER: 466-06-8
RTECS: UK6650000
PURITY: ≥90%
APPEARANCE: White to light yellow powder.
SOLUBILITY: Soluble in methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: TOXIC.
IDENTITY: Determined by IR.

Product Description
Cardiac glycoside. Specific Na+/K+-ATPase inhibitor. Shows high cytotoxicity in tumor cells. Very potent immunosuppressor. DNA topoisomerase I & II inhibitor.
Product Specific Literature References
Clinical studies on proscillaridin a new squill glycoside: L. Gould, et al.; J. Clin. Pharmacol. New Drugs 11, 135 (1971) Abstract
Proscillaridin A immunoreactivity: a new endogenous cardiac glycoside?: S.Q. Li, et al.; Ann. N Y Acad. Sci. 834, 646 (1997) Abstract
Cytotoxicity of digitoxin and related cardiac glycosides in human tumor cells: S. Johansson, et al.; Anticancer Drugs 12, 475 (2001) Abstract
Inhibition of DNA topoisomerases I and II, and growth inhibition of breast cancer MCF-7 cells by ouabain, digoxin and proscillaridin A: K. Bielawski, et al.; Biol. Pharm. Bull. 29, 1493 (2006) Abstract
Apoptosis-mediated cytotoxicity of ouabain, digoxin and proscillaridin A in the estrogen independent MDA-MB-231 breast cancer cells: K. Winnicka, et al.; Arch. Pharm. Res. 30, 1216 (2007) Abstract
Further Categories Containing This Product:
Natural Products - Topoisomerase Inhibitors • Na+ / K+-ATPase / Related Products • Natural Products - Immunomodulators
 
 
ALX-380-299 Revised 29-Jul-08 New product
Prulifloxacin
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SYNONYMS 6-Fluoro-1-methyl-7-[4-[(5-methyl-2-oxo-1,3-dioxol-4-yl)methyl]-1-piperazinyl]-4-oxo-1H,4H-[1,3]thiazeto[3,2-a]quinoline-3-carboxylic acid
NM441
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ALX-380-299-M250   250 mg 90.00 USD Add To Cart
ALX-380-299-G001   1 g 270.00 USD Add To Cart
Product Specification
FORMULA: C21H20FN3O6S
MW: 461.5
CAS NUMBER: 123447-62-1
MERCK INDEX: 14: 7908
PURITY: ≥98% (Assay)
APPEARANCE: White to yellow crystalline solid.
SOLUBILITY: Slightly soluble in dimethyl formamide, 0.1M NaOH or glacial acetic acid; insoluble in water or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Fluoroquinolone with broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria.
Product Specific Literature References
In vivo evaluation of NM441, a new thiazeto-quinoline derivative: M. Ozaki, et al.; Antimicrob. Agents Chemother. 35, 2496 (1991) Abstract; Full Text
Studies on pyridonecarboxylic acids. 1. Synthesis and antibacterial evaluation of 7-substituted-6-halo-4-oxo-4H-[1,3]thiazeto[3,2-a]quinoline-3- carboxylic acids: J. Segawa, et al.; J. Med. Chem. 35, 4727 (1992) Abstract
Pharmacokinetics and safety of NM441, a new quinolone, in healthy male volunteers: M. Nakashima, et al.; J. Clin. Pharmacol. 34, 930 (1994) Abstract
Randomized, double-blind study of prulifloxacin versus ciprofloxacin in patients with acute exacerbations of chronic bronchitis: C. Grassi, et al.; Respiration 69, 217 (2002) Abstract; Full Text
Pharmacologic characteristics of prulifloxacin: M.G. Matera; Pulm. Pharmacol. Ther. 19, 20 (2006) Abstract
Prulifloxacin: a new fluoroquinolone for the treatment of acute exacerbation of chronic bronchitis: M. Cazzola, et al.; Pulm. Pharmacol. Ther. 19, 30 (2006) Abstract
Prulifloxacin: a new antibacterial fluoroquinolone: G. Prats, et al.; Expert. Rev. Anti. Infect. Ther. 4, 27 (2006), (Review) Abstract
Further Categories Containing This Product:
Antibiotics - Topoisomerase Inhibitors • Antibiotics - DNA Replication Inhibitors
 
 
ALX-350-364 Revised 08-Apr-08
Psoralen
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SYNONYMS Ficusin
7H-Furo[3,2-g]benzopyran-7-one
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products with Antibiotic Activity
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ALX-350-364-M025   25 mg 95.00 USD Add To Cart
ALX-350-364-M100   100 mg 285.00 USD Add To Cart
Product Specification
FORMULA: C11H6O3
MW: 186.2
CAS NUMBER: 66-97-7
MERCK INDEX: 14: 7928
RTECS: LV0944000
SOURCE/HOST: Isolated from Psoralea corylifolia.
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in ethyl acetate or acetone; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: MAY BE MUTAGENIC. HARMFUL.

Product Description
Shows antiviral, antibacterial, antifungal and insecticidal properties. Binds DNA through single and double-stranded cross-linking after photoactivation. Used as photochemical probe in studies of DNA mutation and repair mechanisms. Causes photosensitization in human.
Product Specific Literature References
Psoralen phototoxicity: correlation with serum and epidermal 8-methoxypsoralen and 5-methoxypsoralen in the guinea pig: A. Kornhauser, et al.; Science 217, 733 (1982) Abstract
Psoralens as photoactive probes of nucleic acid structure and function: organic chemistry, photochemistry, and biochemistry: G.D. Cimino, et al.; Annu. Rev. Biochem. 54, 1151 (1985), (Review) Abstract
Electron microscopic identification of supercoiled regions in complex DNA structures: R.B. Inman & M. Schnos; J. Mol. Biol. 193, 377 (1987) Abstract
Probing DNA structure with psoralen in vitro: D.W. Ussery, et al.; Meth. Enzymol. 212, 242 (1992) Abstract
DNA interstrand cross-links induced by psoralen are not repaired in mammalian mitochondria: C. Cullinane & V.A. Bohr; Cancer Res. 58, 1400 (1998) Abstract; Full Text
Psoralen photocrosslinking, a tool to study the chromatin structure of RNA polymerase I--transcribed ribosomal genes: M. Toussaint, et al.; Biochem. Cell Biol. 83, 449 (2005) Abstract
Psoralen-induced DNA adducts are substrates for the base excision repair pathway in human cells: S. Couve-Privat, et al.; Nucl. Acids Res. 35, 5672 (2007) Abstract; Full Text
Site-directed gene mutation at mixed sequence targets by psoralen-conjugated pseudo-complementary peptide nucleic acids: K.H. Kim, et al.; Nucl. Acids Res. 35, 7604 (2007) Abstract; Full Text
Further Categories Containing This Product:
Natural Products - Antiviral / anti-HIV Agents • Natural Products - Antifungal Agents • DNA Intercalators & Crosslinkers • Pesticides / Related Products
 
 
ALX-380-236 Revised 08-Apr-08
Quinaldopeptin
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SYNONYMS Antibiotic BMY 28662
Antibiotic BU 3845T
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Other Antibiotics - DNA Regulation / Transcription
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ALX-380-236-MC05   0.5 mg 390.00 USD Add To Cart
Product Specification
FORMULA: C62H78N14O14
MW: 1243.4
CAS NUMBER: 130743-07-6
SOURCE/HOST: Isolated from Amycolatopsis sp. MST-AS5902.
PURITY: ≥95% (HPLC).
APPEARANCE: Yellow powder.
SOLUBILITY: Soluble in methanol, dimethyl formamide or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Antibiotic. DNA bisintercalating agent. Displays antimicrobial, antifungal and cytotoxic properties. Shows antitumor activity in vitro and in vivo.
Product Specific Literature References
Quinaldopeptin, a novel antibiotic of the quinomycin family: S. Toda, et al.; J. Antibiot. (Tokyo) 43, 796 (1990) Abstract
Structure of actinotetraose hexatiglate, a unique glucotetraose from an actinomycete bacterium: R.W. Rickards, et al.; J. Antibiot. (Tokyo) 51, 1093 (1998) Abstract; Full Text
Bisintercalator natural products with potential therapeutic applications: isolation, structure determination, synthetic and biological studies: S. Dawson, et al.; Nat. Prod. Rep. 24, 109 (2007) Abstract
Further Categories Containing This Product:
DNA Intercalators & Crosslinkers • Antibiotics - Antifungal • Antitumor Antibiotics
 
 
ALX-201-233 Revised 22-Jun-07
Rad9/Rad1/Hus1 (human) (recombinant)
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SYNONYMS 9-1-1 Complex (human) (recombinant)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY DNA Damage Checkpoint Other Products
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ALX-201-233-C005   5 µg 240.00 USD Add To Cart
Product Specification
MW: 106kDa (heterotrimer of 43, 31 and 32kDa)
SOURCE/HOST: Produced in baculovirus.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (by SDS-PAGE).
FORMULATION: Liquid. In 25mM Tris, pH 8.0, containing 20% glycerol, 100mM sodium chloride and 1mM β-mercaptoethanol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair: M. Toueille, et al.; Nucl. Acids Res. 32, 3316 (2004) Abstract; Full Text
The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery: E. Smirnova, et al.; Biochem. J. 389, 13 (2005) Abstract
General Literature References
Regulation of the DNA replication fork: a way to fight genomic instability: M. Toueille and U. Hubscher; Chromosoma 113, 113 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Replication Other Products
 
 
ALX-380-092 Revised 03-Jul-08
Radicicol
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SYNONYMS Monorden
1aS-(1aR*,2Z,4E,14*,15aR*)]-8-Chloro-1a,14,15,15a-tetrahydro-9,11-dihydroxy-14-methyl-6H-oxireno[e][2]benzoxacyclotetradecin-6,12(7H)-dione
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Antifungal
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ALX-380-092-M001   1 mg 75.00 USD Add To Cart
ALX-380-092-M005   5 mg 295.00 USD Add To Cart
Product Specification
FORMULA: C18H17ClO6
MW: 364.8
CAS NUMBER: 12772-57-5
MERCK INDEX: 14: 6253
RTECS: RR1105000
SOURCE/HOST: Isolated from Humicola fuscoatra.
PURITY: ≥98% (HPLC)
APPEARANCE: White to light yellow powder.
SOLUBILITY: Soluble in DMSO, methanol or acetone.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
HAZARD: MAY BE CARCINOGENIC. HARMFUL.

Product Description
Antifungal macrocyclic lactone antibiotic with antimalarial activity. Potent inhibitor of HSP90. Binds more strongly to HSP90 (nanomolar affinity) than to Grp94. Also binds to yeast HSP90, E. coli HtpG and TRAP-1. Non-competitive inhibitor of ATP citrate lyase. Anti-angiogenic. Specifically inhibits the interaction between HIF-1α/Arnt heterodimer and the hypoxia-responsive element (HRE), reducing VEGF expression. Protein tyrosine kinase inhibitor, e.g. p60v-src (IC50=0.27nM). Inhibitor of cyclooxygenase-2 (COX-2) expression (IC50=27nM) without affecting COX-1 expression in LPS-stimulated macrophages. Induces the differentiation of HL-60 cells into macrophages, blocking cell cycle at G1 and G2. Suppressor of NIH 3T3 cell transformation by diverse oncogenes such as mos, ras and src in part by blocking the key signal transduction intermediates such as MAP kinase and GAP-associated p62. Inhibitor of AP-1-, NF-κB- and serum response factor (SRF)-mediated transcription (e.g. expression of iNOS). Represses the transcriptional function of the estrogen receptor. Inhibits archeal growth and DNA topoisomerase VI (a Topo IIB family topoisomerase). Blocks replication of negative-strand RNA viruses.
Product Specific Literature References
Potent and specific inhibition of p60v-src protein kinase both in vivo and in vitro by radicicol: H.J. Kwon, et al.; Cancer Res. 52, 6926 (1992) Abstract
Radicicol, a microbial cell differentiation modulator, inhibits in vivo angiogenesis: T. Oikawa, et al.; Eur. J. Pharmacol. 241, 221 (1993) Abstract
Radicicol, a protein tyrosine kinase inhibitor, suppresses the expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide and in experimental glomerulonephritis: P. Chanmugam, et al.; J. Biol. Chem. 270, 5418 (1995) Abstract; Full Text
Suppression of RAS and MOS transformation by radicicol: J.F. Zhao, et al.; Oncogene 11, 161 (1995) Abstract
Induction of differentiation of HL-60 cells by the anti-fungal antibiotic, radicicol: Y. Shimada, et al.; J. Antibiot. 48, 824 (1995) Abstract
Radicicol inhibits tyrosine phosphorylation of the mitotic Src substrate Sam68 and retards subsequent exit from mitosis of Src-transformed cells: I. Pillay, et al.; Cell Growth Differ. 7, 1487 (1996) Abstract
Radicicol leads to selective depletion of Raf kinase and disrupts K-Ras-activated aberrant signaling pathway: S. Soga, et al.; J. Biol. Chem. 273, 822 (1998) Abstract; Full Text
Antimalarial activity of radicicol, heptelidic acid and other fungal metabolites: Y. Tanaka, et al.; J. Antibiot. 51, 153 (1998) Abstract
Targeting of the protein chaperone, HSP90, by the transformation suppressing agent, radicicol: S.V. Sharma, et al.; Oncogene 16, 2369 (1998) Abstract
Structural basis for inhibition of the Hsp90 molecular chaperone by the antitumor antibiotics radicicol and geldanamycin: S.M. Roe, et al.; J. Med. Chem. 42, 260 (1999) Abstract
T.W. Schulte, et al.; Mol. Endocrinol. 13, 1435 (1999) Abstract; Full Text
Radicicol suppresses expression of inducible nitric-oxide synthase by blocking p38 kinase and nuclear factor-kappaB/Rel in lipopolysaccharide-stimulated macrophages: Y.J. Jeon, et al.; J. Pharmacol. Exp. Ther. 294, 548 (2000) Abstract; Full Text
Radicicol binds and inhibits mammalian ATP citrate lyase: S.W. Ki, et al.; J. Biol. Chem. 275, 39231 (2000) Abstract; Full Text
Radicicol suppresses transformation and restores tropomyosin-2 expression in both ras- and MEK-transformed cells without inhibiting the Raf/MEK/ERK signaling cascade: P.N. Kim, et al.; Cell Growth Differ. 12, 543 (2001) Abstract; Full Text
Radicicol-sensitive peptide binding to the N-terminal portion of GRP94: S. Vogen, et al.; J. Biol. Chem. 277, 40742 (2002) Abstract; Full Text
Reduction of hypoxia-induced transcription through the repression of hypoxia-inducible factor-1alpha/aryl hydrocarbon receptor nuclear translocator DNA binding by the 90-kDa heat-shock prot