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NF-kB Transcription Factors / Related Products
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ALX-804-043 Revised 04-Dec-04
Monoclonal Antibody to NF-κB (human) (NF3A11)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-043-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: NF3A11
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: 100µg of protein G purified antibody in 100µl Tris/Glycine buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p50 subunit).
SPECIFICITY: Recognizes p50 subunit of human NF-κB (epitope in the region aa 191-197).
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-044 Revised 04-Dec-04
Monoclonal Antibody to NF-κB (human) (NF5E6)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-044-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: NF5E6
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein G purified antibody in Tris/Glycine buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p50 subunit).
SPECIFICITY: Recognizes p50 subunit of human NF-κB (epitope in the region aa 1-15).
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-361 Revised 09-May-07
Monoclonal Antibody to NF-κB (p65) (112A1021)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-361-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 112A1021
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 526-539 of human NF-κB (p65 subunit) .
SPECIFICITY: Recognizes human and mouse NF-κB (p65 subunit ). Detects a band of ~65kDa by Western blot.
APPLICATION: Flow Cyctometry (2.5µg/ml) (see [1])
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Specific Literature References
[1] Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases: M. Wittmann, et al.; J. Invest. Dermatol. 124, 1225 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-201-285 Revised 09-Oct-08
NF-κB (p50) (human) (recombinant) (His)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-285-C002   2 µg 280.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.2mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 125mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P19838: NF-κB (human) (p50 subunit)
AfCS Signalling Gateway link A002937: NF-κB (mouse) (p50 subunit)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-284 Revised 18-Mar-08
NF-κB (p65) (human) (recombinant) (His)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-284-C002   2 µg 280.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression: M. Covic, et al.; EMBO J. 24, 85 (2005) Abstract; Full Text
MYBBP1a is a novel repressor of NF-κB: H. R. Owen, et al.; J. Mol. Biol. 366, 725 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q04206: NF-κB (human) (p65 subunit)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-210-576 Revised 18-Oct-07
Polyclonal Antibody to NF-κB (p50)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-576-R100   100 µl 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p50 subunit).
SPECIFICITY: Recognizes human NF-κB (p50 subunit).
APPLICATION: Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for one week when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-574 Revised 18-Oct-07
Polyclonal Antibody to NF-κB (p65)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-574-R100   100 µl 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p65 subunit).
SPECIFICITY: Recognizes human and mouse NF-κB (p65 subunit).
APPLICATION: Immunocytochemistry (1:500-1:1’000)
Immunohistochemistry (paraffin sections (1:500-1:1’000))
Immunoprecipitation (2µg)
Western Blot (1:2’000-1:5’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for one week when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 

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