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Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-350-268 Revised 01-Jun-06
Phalloidin (FITC)
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SYNONYMS Fluoresceinyl-aminomethyldithiolano-phalloidin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products for Cytoskeletal Research
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ALX-350-268-MC01   0.1 mg 150.00 USD Add To Cart
Product Specification
FORMULA: C58H62N10O14S4
MW: 1251.4
SOURCE/HOST: Semisynthetic from aminomethyldithiolano-phalloidin.
PURITY: ≥90%
APPEARANCE: Amorphous solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, methanol or water (warm).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Prepare fresh solutions for each application.
HANDLING: Protect from light and moisture.
HAZARD: VERY TOXIC.

Product Description
For detection of microfilaments in the light microscope. Mixture of 4 isomers with lower affinity to actin than phalloidin (Prod. No. ALX-350-265).
Product Specific Literature References
Fluorescent phallotoxin, a tool for the visualization of cellular actin: E. Wulf, et al.; PNAS 76, 4498 (1979) Abstract
Fluorescent phallotoxins as probes for filamentous actin: H. Faulstich, et al.; J. Muscle Res. Cell Motil. 9, 370 (1988) Abstract
Further Categories Containing This Product:
Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Amanitins & Phalloidins/Related Products • Actin/Related Products
 
 
ALX-400-043 Revised 09-May-07
Propidium Iodide
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-400-043-M001   1 mg 77.00 USD Add To Cart
Product Specification
FORMULA: C27H34N4I2
MW: 668.4
CAS NUMBER: 25535-16-4
RTECS: SF7949600
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Solution in PBS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.

Product Description
DNA intercalator. Useful for differentiation between apoptotic cell death if used in combination with annexin V conjugates by flow cytometry. Ex(max): 536nm; Em(max): 617nm.
Product Specific Literature References
Propidium iodide staining correlates with the extent of DNA degradation in isolated nuclei: T. Crompton, et al.; BBRC 183, 532 (1992) Abstract
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells: F. Belloc, et al.; Cytometry 17, 59 (1994) Abstract
Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide: O. Honda, et al.; Int. J. Oncol. 16, 283 (2000) Abstract
Flow cytometric analysis of apoptotic subpopulations with a combination of annexin V-FITC, propidium iodide, and SYTO 17: M. Eray, et al.; Cytometry 43, 134 (2001) Abstract
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
Distribution of the human intracellular serpin protease inhibitor 8 in human tissues: M.C. Strik, et al.; J. Histochem. Cytochem. 50, 1443 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Dilute to 250µg/ml in PBS before use. Add 1µl per annexin V assay.
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Further Categories Containing This Product:
DNA Intercalators & Crosslinkers
 
 
ALX-620-006 Revised 04-Mar-05
QUIN 2 . tetrapotassium salt
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SYNONYMS 2-[(2-bis-[Carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis[carboxymethyl]aminoquinoline . 4K
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Chelators/Caged Calcium Chelators
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ALX-620-006-M005   5 mg 35.00 USD Add To Cart
Product Specification
FORMULA: C26H23N3O10 . 4K
MW: 537.5 . 156.4
CAS NUMBER: 73630-23-6
MERCK INDEX: 14: 8042
PURITY: ≥95% (contains 11-13% water)
APPEARANCE: Yellow powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Calcium specific chelator and indicator. Upon binding calcium there is a shift in the UV spectrum and an enhancement of fluorescence. QUIN 2 was a member of the first generation of Ca2+ chelators introduced by Tsien in 1980. These chelators have backbones similar to that of EGTA (ethylenglycol-bis(β-aminoethyl)-N,N,N',N'-tetraacetic acid) and, like EGTA, they are able to chelate Ca2+ with high affinity and specifity. Moreover, these molecules contain aromatic rings that interact electronically and sterically with the chelating backbone. Thus chelation results in changes in the UV-absorbing and fluorescing properties of the molecules. The resulting spectral changes allow these substances to be used as very sensitive probes for Ca2+ ions, Ca2+ concentrations in single cells of various types can be measured for the first time! TPEN (Prod. No. ALX-450-011) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.
Product Specific Literature References
Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator: R.Y. Tsien, et al.; J. Cell. Biol. 94, 325 (1982) Abstract
Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas. A new, membrane-permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free Ca2+: P. Arslan, et al.; J. Biol. Chem. 260, 2719 (1985) Abstract; Full Text
Calcium transients in human platelets monitored by aequorin, fura-2 and quin-2: effects of protein kinase C activation and inhibition: P. Erne, et al.; BBRC 145, 66 (1987) Abstract
Effect of DFP on loading of fura 2/AM and quin 2/AM into single smooth muscle cells prepared from guinea pig taenia coli: I. Maruyama, et al.; FEBS Lett. 220, 89 (1987) Abstract
Effects of interferon on natural killer (NK) cells assessed by fluorescent probes and flow cytometry: K. McGinnes, et al.; J. Immunol. Meth. 107, 129 (1988) Abstract
Measurement and manipulation of cytoplasmic free calcium of ram and boar spermatozoa using quin 2: A.M. Simpson & I.G. White; Cell Calcium 9, 45 (1988) Abstract
Measurement of the cytosolic free calcium ion concentration of individual lymphocytes by microfluorometry using quin 2 or fura-2: H. Komada, et al.; Cell Struct. Funct. 14, 141 (1989) Abstract
Quantitation of bovine sperm cytoplasmic calcium with Quin-2 and Fura- 2: evidence that external calcium does not have direct access to the sperm cytoplasm: S. Vijayaraghavan & D. Hoskins; Cell Calcium 10, 241 (1989) Abstract
Further Categories Containing This Product:
MDR Other Products • Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Calcium Indicators/Probes
 
 
ALX-550-293 Revised 19-Mar-08
Reactive Blue 2
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SYNONYMS Basilen Blue E-3G
PRODUCT LINE Neurobiology
PRODUCT CATEGORY P2Y Purinergic Receptors/Related Products
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ALX-550-293-G005   5 g 15.00 USD Add To Cart
Product Specification
FORMULA: C29H17ClN7O11S3 . Na3
MW: 771.1 . 69.0
CAS NUMBER: 12236-82-7
APPEARANCE: Dark blue solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.
IDENTITY: Determined by IR and UV.

Product Description
Dye with the mixture of isomers (m/p). Potent inhibitor of ATP-activated channels. Blocks P2Y receptors. Can also be used for probing nucleotide binding sites in numerous proteins.
Product Specific Literature References
Applications of blue dextran and Cibacron Blue F3GA in purification and structural studies of nucleotide-requiring enzymes: J.E. Wilson; BBRC 72, 816 (1976) Abstract
Analytical evaluation of the purity of commercial preparations of Cibacron Blue F3GA and related dyes: D. Hanggi & P. Carr; Anal. Biochem. 149, 91 (1985) Abstract
Adenosine triphosphate-evoked currents in cultured neurones dissociated from rat parasympathetic cardiac ganglia: L.A. Fieber & D.J. Adams; J. Physiol. 434, 239 (1991) Abstract
Antagonism by reactive blue 2 but not by brilliant blue G of extracellular ATP-evoked responses in PC12 phaeochromocytoma cells: K. Inoue, et al.; Br. J. Pharmacol. 102, 851 (1991) Abstract
Effects of ATP antagonists on purinoceptor-operated inward currents in rat phaeochromocytoma cells: K. Nakazawa, et al.; Pfluegers Arch. 418, 214 (1991) Abstract
Pharmacology and electrophysiology of ATP-activated ion channels: B.P. Bean; TIPS 13, 87 (1992), (Review) Abstract
Further Categories Containing This Product:
MDR Other Products • Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Ion Channels & Transporters Other Products
 
 
ALX-850-285 Revised 04-Mar-05
RedPack-NHS
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-850-285-KI01   1 Kit 590.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Kit contains 1mg each of DY-630-NHS, DY-635-NHS, DY-640-NHS, DY-650-NHS, DY-655-NHS, DY-656-NHS and EVOblue™ 30-NHS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
The DY-labels of the RedPAck are amino-reactive fluorescent biolabels designed for excitation by red lasers (He/Ne; 633nm) and diode lasers (635-650nm). They can be used for covalent coupling to proteins and other biomolecules containing primary amino groups like amino-modified DNA-oligomers and amino-modified biotin. The chromophores responsible for the absorption maxima are based on bridged hemicyanines and merocyanines.
General Information
MANUFACTURER Manufactured by Dyomics GmbH.
Further Categories Containing This Product:
Panels/Sets
 
 
ALX-620-008 Revised 22-Dec-06
RHOD 2 . triammonium salt
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SYNONYMS 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid . 3NH4+
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Chelators/Caged Calcium Chelators
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ALX-620-008-M001   1 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C40H39N4O11 . 3NH4+
MW: 751.7 . 54.1
CAS NUMBER: 129787-65-1
PURITY: ≥90%
SOLUBILITY: Soluble in water (pH >6) or DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light and moisture.

Product Description
A cell impermeable, UV excitable Ca2+-specific fluorophore. Can be loaded into cells via microinjection or scrape loading. Excitation and emission spectra do not undergo a shift and the sensor is essentially nonfluorescent before Ca2+-binding but becomes more fluorescent with increasing Ca2+ concentration. Due to its longer λmax. the product is useful for applications where autofluoresence is a problem, or where another fluorescent dye of shorter λmax. is used at the same time. Fluorescent enhancement from low to high [Ca2+] was smaller than that for FLUO 3 (Prod. No. ALX-620-002), and also in general the fluorescence is somewhat less than of FLUO 3.
Product Specific Literature References
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores: A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) Abstract; Full Text
Effects of the N-methyl-D-aspartate antagonists on the rise in [Ca2+]i following depolarization in aged rat brain synaptosomes: M. Okada, et al.; Brain Res. 583, 227 (1992) Abstract
Cyclic AMP-dependent phosphorylation of the inositol-1,4,5- trisphosphate receptor inhibits Ca2+ release from platelet membranes: T.M. Quinton & W.L. Dean; BBRC 184, 893 (1992) Abstract
Mechanisms of cholecystokinin-induced protection of cultured cortical neurons against N-methyl-D-aspartate receptor-mediated glutamate cytotoxicity: Y. Tamura, et al.; Brain Res. 592, 317 (1992) Abstract
Confocal microscopy reveals coordinated calcium fluctuations and oscillations in synaptic boutons: N. Melamed, et al.; J. Neurosci. 13, 632 (1993) Abstract
Further Categories Containing This Product:
MDR Other Products • Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Calcium Indicators/Probes
 
 
ALX-620-009 Revised 24-May-07
RHOD 2/AM
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SYNONYMS 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N’,N’-tetraacetic acid, tetraacetoxy methyl ester . Br
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calcium Chelators/Caged Calcium Chelators
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ALX-620-009-M001   1 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C52H59N4O19 . Br
MW: 1044.1 . 79.9
CAS NUMBER: 129787-64-0
PURITY: ≥90% (HPLC)
APPEARANCE: Red solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light and moisture.

Product Description
Membrane permeable form of RHOD 2 (Prod. No. ALX-620-008). Fluorescent probe for intracellular Ca2+.
Product Specific Literature References
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores: A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) Abstract; Full Text
Effects of the N-methyl-D-aspartate antagonists on the rise in [Ca2+]i following depolarization in aged rat brain synaptosomes: M. Okada, et al.; Brain Res. 584, 227 (1992) Abstract
Cyclic AMP-dependent phosphorylation of the inositol-1,4,5- trisphosphate receptor inhibits Ca2+ release from platelet membranes: T.M. Quinton & W.L. Dean; BBRC 184, 893 (1992) Abstract
Mechanisms of cholecystokinin-induced protection of cultured cortical neurons against N-methyl-D-aspartate receptor-mediated glutamate cytotoxicity: Y. Tamura, et al.; Brain Res. 592, 317 (1992) Abstract
Confocal microscopy reveals coordinated calcium fluctuations and oscillations in synaptic boutons: N. Melamed, et al.; J. Neurosci. 13, 632 (1993) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Membrane permeable form may be loaded into cells directly via incubation. A mild detergent (e.g. Pluronic F-127) may be used to facilitate the loading process. RHOD 2/AM itself does not bind Ca2+, but once inside the cell it is readily hydrolyzed to RHOD 2 by endogenous esterases.

Includes 1 vial of Pluronic® (F127).
Further Categories Containing This Product:
Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Calcium Indicators/Probes • MDR Other Products
 
 
ALX-610-024 Revised 30-Mar-07
Rhodamine 6G . chloride
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-610-024-G001   1 g 10.00 USD Add To Cart
ALX-610-024-G005   5 g 25.00 USD Add To Cart
Product Specification
FORMULA: C28H31N2O3 . Cl
MW: 443.6 . 35.5
CAS NUMBER: 989-38-8
PURITY: ≥95%
APPEARANCE: Brown-red powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
HAZARD: IRRITANT.

Product Description
Cell permeable fluorescent probe (Ex(max): 528nm; Em(max): 551nm) used to examine the efficiency of P-glycoprotein (Pgp) mediated exclusion from multidrug-resistant cells.
Product Specific Literature References
Relevance of the chemical charge of rhodamine dyes to multiple drug resistance: T.J. Lampidis, et al.; Biochem. Pharmacol. 38, 4267 (1989) Abstract
Novel screening method for agents that overcome classical multidrug resistance in a human cell line: A. Yoshimura, et al.; Cancer Lett. 50, 45 (1990) Abstract
In vivo characterization of the drug resistance profile of the major ABC transporters and other components of the yeast pleiotropic drug resistance network: M. Kolaczkowski, et al.; Microb. Drug Resist. 4, 143 (1998) Abstract
Rhodamine 6G efflux for the detection of CDR1-overexpressing azole- resistant Candida albicans strains: S. Maesaki, et al.; J. Antimicrob. Chemother. 44, 27 (1999) Abstract
Further Categories Containing This Product:
P-glycoprotein [Pgp]/Related Products
 
 
ALX-610-018 Revised 01-Apr-08
Rhodamine 123 . hydrochloride
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SYNONYMS 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester . chloride
PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-610-018-M005   5 mg 20.00 USD Add To Cart
Product Specification
FORMULA: C21H16N2O3 . HCl
MW: 344.4 . 36.5
CAS NUMBER: 62669-70-9
PURITY: ≥96% (HPLC)
APPEARANCE: Red-brown powder.
SOLUBILITY: Soluble in 100% ethanol (1mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
A lipophilic cationic fluorescent dye (Ex(max): 507nm; Em(max): 529nm), which selectively locates mitochondria in living cells [1]. Relatively non-toxic [1,2]. Effluxed efficiently by MDR cells [3,4] which is inhibited by established modulators of MDR [4,5]. By following cellular rhodamine 123 accumulation or retention in the presence or absence of P-glycoprotein (Pgp) modulators, gives valuable information on Pgp activity and efficacy of these modulators in reversing Pgp activity can be obtained [6-9]. Since rhodamine 123 is considered a permeant cationic fluorescent probe [2], its uptake into cells has generally been assumed to occur via a passive diffusion process [10]. Recently it was suggested that performing rhodamine 123 accumulation studies without first ascertaining a possible role for a carrier system for cellular uptake of rhodamine 123 and putative Pgp modulators might inadvertently lead to draw improper conclusions on Pgp activity [11].
Product Specific Literature References
[1] Localization of mitochondria in living cells with rhodamine 123: L.V. Johnson, et al.; PNAS 77, 990 (1980) Abstract
[2] Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy: L.V. Johnson, et al.; J. Cell. Biol. 88, 526 (1981) Abstract
[3] Cross-resistance to rhodamine 123 in Adriamycin- and daunorubicin- resistant Friend leukemia cell variants: H. Tapiero, et al.; Cancer. Res. 44, 5544 (1984) Abstract
[4] Use of fluorescent dyes as molecular probes for the study of multidrug resistance: A.A. Neyfakh; Exp. Cell. Res. 174, 168 (1988) Abstract
[5] Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells: T.J. Lampidis, et al.; Cancer. Res. 45, 2626 (1985) Abstract
[6] Detection of activity of P-glycoprotein in human tumour samples using rhodamine 123: C. Ludescher, et al.; Br. J. Haematol. 82, 161 (1992) Abstract
[7] Characterization of rhodamine 123 binding to P-glycoprotein in human multidrug-resistant cells: B. Nare, et al.; Mol. Pharmacol. 45, 1145 (1994) Abstract
[8] A comparison of rhodamine 123 accumulation and efflux in cells with P- glycoprotein-mediated and MRP-associated multidrug resistance phenotypes: P.R. Twentyman, et al.; Eur. J. Cancer. 30A, 1360 (1994) Abstract
[9] Stoichiometry of coupling of rhodamine 123 transport to ATP hydrolysis by P-glycoprotein: A.B. Shapiro & V. Ling; Eur. J. Biochem. 254, 189 (1998) Abstract
[10] Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane: G.A. Altenberg, et al.; PNAS 91, 4654 (1994) Abstract
[11] Carrier-mediated uptake of rhodamine 123: implications on its use for MDR research: C.W. Cho, et al.; BBRC 279, 124 (2000) Abstract
[12] Altered multidrug resistance phenotype caused by anthracycline analogues and cytosine arabinoside in myeloid leukemia: X.F. Hu, et al.; Blood 93, 4086 (1999) Abstract
Further Categories Containing This Product:
P-glycoprotein [Pgp]/Related Products
 
 
ALX-550-295 Revised 08-Jul-08
Ruthenium red
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PRODUCT LINE Other Products
PRODUCT CATEGORY