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ALX-804-530
Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-530-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
1H11
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death:
V.S. Marsden, et al.; Blood
107
, 1872 (2006)
Abstract
;
Full Text
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-531
Revised 10-Mar-06
Monoclonal Antibody to Caspase-1 (mouse) (4G8)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-531-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
4G8
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
Product Images
Please click on thumbnails to enlarge.
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-627
Revised 31-Oct-06
Monoclonal Antibody to c-Myc (human) (4H3)
SYNONYMS
anti-Myc Proto-oncogene (human) MAb (4H3)
anti-Transcription Factor p64 (human) MAb (4H3)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-627-L001
1 ml
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
4H3
ISOTYPE:
Rat IgG2a
FORMULATION:
Liquid. Tissue culture supernatant (TCS) containing 0.1% sodium azide.
IMMUNOGEN:
Recombinant human c-Myc (aa 1-262).
SPECIFICITY:
Recognizes human and zebrafish c-Myc.
APPLICATION:
Immunocytochemistry (1:10)
Immunohistochemistry (frozen sections)
- tested only in zebrafish!
Immunoprecipitation (1:10)
Western Blot (1:50)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 1 year when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Identification and characterization of specific DNA-binding complexes containing members of the Myc/Max/Mad network of transcriptional regulators:
A. Sommer, et al.; J. Biol. Chem.
273
, 6632 (1998)
Abstract
;
Full Text
General Information
Myc proteins are important regulators of cell behaviour and work as transcription factors that belong to the basic region/helix-loop-helix/leucine zipper family of DNA binding proteins. To regulate its target genes, Myc recruits several different cofactors (e.g. PARP-10). They control a variety of cellular functions including protein synthesis, cell cycle progression, and apoptosis, thereby modulating cell growth and proliferation, differentiation, and survivial of cells. The family of myc genes includes B-myc, L-myc, N-myc and s-myc; however only c-myc, L-myc and N-myc have neoplastic potential.
The c-Myc gene was discovered as the cellular homolog of the retroviral v-myc oncogene. It is a proto-oncogene, whereas the deregulated expression of c-Myc is observed in the majority of human and animal tumors often with poor prognosis.
The proto-oncogene c-Myc is implicated in various physiological processes; cell growth, proliferation, loss of differentiation and cell death (apoptosis). It also has been implicated in the loss and dysfunction of insulin-producing beta cells in diabetes. Recent studies in mice suggest that c-Myc has unexpected functions during both self-renewal and the differentiation of stem and early progenitor cells. c-Myc activity is normally tightly controlled by external signals including growth factors, mitogens and beta-catenin which promote, and factors as TGF-beta which inhibit.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-632
Revised 31-Oct-06
Monoclonal Antibody to c-Myc (human) (6A10)
SYNONYMS
anti-Myc Proto-oncogene (human) MAb (6A10)
anti-Transcription Factor p64 (human) MAb (6A10)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-632-L001
1 ml
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
6A10
ISOTYPE:
Rat IgG2a
FORMULATION:
Liquid. Tissue culture supernatant (TCS) containing 0.1% sodium azide.
IMMUNOGEN:
Human c-Myc (aa 1-262).
SPECIFICITY:
Recognizes human c-Myc.
APPLICATION:
Immunocytochemistry (1:10)
Immunoprecipitation (1:10)
Western Blot (1:50)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 1 year when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Identification and characterization of specific DNA-binding complexes containing members of the Myc/Max/Mad network of transcriptional regulators:
A. Sommer, et al.; J. Biol. Chem.
273
, 6632 (1998)
Abstract
;
Full Text
Inhibition of proliferation and apoptosis by the transcriptional repressor Mad1. Repression of Fas-induced caspase-8 activation:
S. Gehring, et al.; J. Biol. Chem.
275
, 10413 (2000)
Abstract
;
Full Text
PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation:
M. Yu, et al.; Oncogene
24
, 1982 (2005)
Abstract
General Information
Myc proteins are important regulators of cell behaviour and work as transcription factors that belong to the basic region/helix-loop-helix/leucine zipper family of DNA binding proteins. To regulate its target genes, Myc recruits several different cofactors (e.g. PARP-10). They control a variety of cellular functions including protein synthesis, cell cycle progression, and apoptosis, thereby modulating cell growth and proliferation, differentiation, and survivial of cells. The family of myc genes includes B-myc, L-myc, N-myc and s-myc; however only c-myc, L-myc and N-myc have neoplastic potential.
The c-Myc gene was discovered as the cellular homolog of the retroviral v-myc oncogene. It is a proto-oncogene, whereas the deregulated expression of c-Myc is observed in the majority of human and animal tumors often with poor prognosis.
The proto-oncogene c-Myc is implicated in various physiological processes; cell growth, proliferation, loss of differentiation and cell death (apoptosis). It also has been implicated in the loss and dysfunction of insulin-producing beta cells in diabetes. Recent studies in mice suggest that c-Myc has unexpected functions during both self-renewal and the differentiation of stem and early progenitor cells. c-Myc activity is normally tightly controlled by external signals including growth factors, mitogens and beta-catenin which promote, and factors as TGF-beta which inhibit.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-389
Revised 30-May-08
Monoclonal Antibody to CREB (pSer133) and ATF1 (pSer63) (phosphorylated) (10E9)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
CREB & CREB Binding Protein/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-389-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
10E9
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic peptide corresponding to aa 125-135 of CREB phosphorylated at Ser
133
.
SPECIFICITY:
Recognizes the RRPpSYRK motif of human, mouse or dog CREB phosphorylated at Ser
133
and ATF1 phosphorylated at Ser
63
. Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot.
APPLICATION:
Western Blot
(0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:
yes
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-708
Revised 30-Jan-07
Monoclonal Antibody to FOXP3 (mouse) (MF333F)
SYNONYMS
anti-Forkhead Box Protein P3 (mouse) MAb (MF333F)
anti-Scurfin (mouse) MAb (MF333F)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Forkhead Box [FOX] Proteins/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-708-C050
50 µg
220.00 USD
ALX-804-708-C100
100 µg
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
MF333F
ISOTYPE:
Rat IgG2a
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant mouse FOXP3 (forkhead box protein P3).
SPECIFICITY:
Recognizes both natural and recombinant mouse FOXP3. Detects a band of ~46-49kDa by Western blot.
APPLICATION:
ELISA
(direct and indirect: 1:2’000-1:10’000)
Immunocytochemistry
Immunohistochemistry
(frozen sections) (see [3], [4])
Western Blot
(1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
Immunosurveillance of Erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance:
E. Ambrosino, et al.; Cancer Res.
66
, 7734 (2006)
Abstract
[2]
Regulated compartmentalization of programmed cell death-1 discriminates CD4+CD25+ resting regulatory T cells from activated T cells:
G. Raimondi, et al.; J. Immunol.
176
, 2808 (2006)
Abstract
[3]
Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice:
M.J. Perone, et al.; J. Immunol.
177
, 5278 (2006)
Abstract
[4]
"Alternatively activated" dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig:
Y.Y. Lan, et al.; J. Immunol.
177
, 5868 (2006)
Abstract
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
FOXP3/Related Products
•
Monoclonal Antibodies
ALX-804-216
Revised 31-May-07
Monoclonal Antibody to HIF-1α (mgc3)
SYNONYMS
anti-Hypoxia-inducible Factor 1α MAb (mgc3)
anti-ARNT-interacting Protein MAb (mgc3)
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-216-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
mgc3
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).
SPECIFICITY:
Recognizes human and mouse (only in Gel-Supershift Assays) HIF-1α. Detects a band of ~116kDa by Western blot after hypoxic induction.
Does not cross-react with HIF-1β
or HIF-2
α
.
APPLICATION:
Gel Supershift Assay
Immunocytochemistry (1:200)
Immunoprecipitation
Western Blot (1:2’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobuilins raised against thy hypoxia-inducible factor 1alpha:
G. Camenisch, et al.; FASEB J.
13
, 81 (1999)
Abstract
;
Full Text
[2]
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha:
D. Chilov, et al.; J. Cell. Sci.
112
, 1203 (1999)
Abstract
;
Full Text
General Information
Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.
Further Categories Containing This Product:
Aryl Hydrocarbon Receptor/Related Products
•
Monoclonal Antibodies
ALX-804-209
Revised 27-May-08
Monoclonal Antibody to IκBα (6A920)
SYNONYMS
anti-NF-κB Inhibitor α MAb (6A920)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-209-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
6A920
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human IκBα
(aa 32-391).
SPECIFICITY:
Recognizes both phosphorylated and non-phosphorylated forms of human and mouse IκBα. Detects a band of ~40kDa by Western blot.
APPLICATION:
Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
General Literature References
Two waves of nuclear factor kappaB recruitment to target promoters
:
S. Saccani, et al.; J. Exp. Med.
193
, 1351 (2001)
Abstract
;
Full Text
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment
:
S. Saccani, et al.; Nat. Immunol.
3
, 69 (2002)
Abstract
Modulation of NF-kappaB activity by exchange of dimers
:
S. Saccani, et al.; Mol. Cell
11
, 1563 (2003)
Abstract
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock
:
T. Ikezoe, et al.; Mol. Pharmacol.
64
, 1521 (2003)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-440
Revised 19-Sep-05
Monoclonal Antibody to IκBα (cleaved) (human) (5D1623)
SYNONYMS
anti-NF-κB Inhibitor α (cleaved) (human) MAb (5D1623)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-440-C100
100 µg
319.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
5D1623
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IκBα (NF-κB Inhibitor α).
SPECIFICITY:
Recognizes caspase-3 cleaved human IκBα.
APPLICATION:
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-235
Revised 31-Oct-06
Monoclonal Antibody to IκBα (human) (phosphorylated) (pSer32/36) (39A1431)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
