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DNA Regulation / Transcription
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ALX-210-530 Revised 13-Oct-08
Polyclonal Antibody to DMPO
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-530-R100   100 µl 290.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains no stabilizers or preservatives.
IMMUNOGEN: DMPO-octanoic acid conjugated to ovalbumin.
SPECIFICITY: Recognizes DMPO (Prod. No. ALX-430-090), DMPO-octanoic acid and DMPO-protein adducts. Does not cross react with non-adducted proteins.
APPLICATION: ELISA: 1:5’000. Reactive against DMPO-protein adducts in dilutions of 1:100-10’000 followed by reaction with alkaline phosphatase conjugated, affinity purified anti-rabbit IgG (goat).
Immunocytochemistry
Western Blot: 1:5’000.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Dilute only prior to immediate use.
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Product Specific Literature References
Immunological identification of the heart myoglobin radical formed by hydrogen peroxide: C.D. Detweiler, et al.; Free Radic. Biol. Med. 33, 364 (2002) Abstract
Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: involvement of a protein-tyrosyl radical: D.C. Ramirez, et al.; Free Radic. Biol. Med. 34, 830 (2003) Abstract
UVA-ketoprofen-induced hemoglobin radicals detected by immuno-spin trapping: Y.Y. He, et al.; Photochem. Photobiol. 77, 585 (2003) Abstract
Reaction of human hemoglobin with peroxynitrite. Isomerization to nitrate and secondary formation of protein radicals: N. Romero, et al.; J. Biol. Chem. 278, 44049 (2003) Abstract
Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical: L. Deterding; J. Biol. Chem. 279, 11600 (2004) Abstract
Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping: R.P. Mason; Free Radic. Biol. Med. 36, 1214 (2004) Abstract
Protein radical formation during lactoperoxidase-mediated oxidation of the suicide substrate glutathione: immunochemical detection of a lactoperoxidase radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct: Q. Guo, et al.; J. Biol. Chem. 279, 13272 (2004) Abstract
Involvement of protein radical, protein aggregation, and effects on NO metabolism in the hypochlorite-mediated oxidation of mitochondrial cytochrome c: Y.R. Chen, et al.; Free Radic. Biol. Med. 37, 1591 (2004) Abstract
Novel identification of a sulfur-centered, radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct formed from the oxidation of DTT by LC/ELISA, LC/electrospray ionization-MS, and LC/tandem MS: Q. Guo, et al.; Chem. Res. Toxicol. 17, 1481 (2004) Abstract
Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations: D.C. Ramirez, et al.; Free Radic. Biol. Med. 38, 201 (2005) Abstract
Copper-catalyzed protein oxidation and its modulation by carbon dioxide: enhancement of protein radicals in cells: D.C. Ramirez, et al.; J. Biol. Chem. 280, 27402 (2005) Abstract
Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization: C.D. Detweiler, et al.; Free Radic. Biol. Med. 38, 969 (2005) Abstract
Immuno-spin trapping: detection of protein-centered radicals: D.C. Ramirez, et al.; Curr. Prot. in Toxicology 2, 17.7.1 (2005)
Cytochrome c acts as a cardiolipin oxygenase required for release of proapoptotic factors: V.E. Kagan, et al.; Nat. Chem. Biol. 1, 223 (2005) Abstract
Superoxide generation from mitochondrial NADH dehydrogenase induces self-inactivation with specific protein radical formation: Y.R. Chen, et al.; J. Biol. Chem. 280, 37339 (2005) Abstract; Full Text
Immunochemical detection of nitric oxide and nitrogen dioxide trapping of the tyrosyl radical and the resulting nitrotyrosine in sperm whale myoglobin: K. Nakai and R.P. Mason; Free Radic. Biol. Med. 39, 1050 (2005) Abstract
Immuno-spin trapping of DNA radicals: D.C. Ramirez, et al.; Nat. Methods 3, 123 (2006) Abstract
Immuno-spin trapping of hemoglobin and myoglobin radicals derived from nitrite-mediated oxidation: A. Keszler, et al.; Free Radic. Biol. Med. 40, 507 (2006) Abstract
Immuno-spin trapping: A breakthrough for sensitive detection of protein-derived radicals: R. Radi, et al.; Free Radic. Biol. Med. 41, 416 (2006)
Protein radical formation on thyroid peroxidase during turnover: M. Ehrenshaft, et al.; Free Radic. Biol. Med. 41, 422 (2006)
Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes: M.G. Bonini et al.; Free Rad. Biol. Med. 42, 530 (2007)
Mitochondrial Complex II in the Post-ischemic Heart: Oxidative injury and the role or protein S-Glutathionylation: Y.R. Chen, et al.; J. Biol. Chem. 282, 32640 (2007) Abstract; Full Text
Further Categories Containing This Product:
DNA Damage & Repair Other Products • Polyclonal Antibodies
 
 
ALX-210-532 Revised 31-Oct-06
Polyclonal Antibody to NF-ATc4 (human)
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SYNONYMS anti-Nuclear Factor of Activated T Cells, Cytoplasmic 4 (human) PAb
anti-NFAT3 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Factor of Activated T Cells [NF-AT]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-532-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 887-902 (CR887DLSGFPAPPGEEPPA902) of human NF-ATc4 (nuclear factor of activated T cells, cytoplasmic 4). This sequence is 94% conserved in mouse NF-ATc4.
SPECIFICITY: Recognizes human NF-ATc4. Detects a band of ~150kDa by Western blot.
APPLICATION: Immunohistochemistry (1:3’000)
Western Blot (1:10’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Note: Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells 5 (NF-AT5)
Gene Names: NFAT5
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-533 Revised 31-Oct-06
Polyclonal Antibody to NF-AT5 (human)
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SYNONYMS anti-Nuclear Factor of Activated T Cells 5 (human) PAb
anti-NFAT5 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Factor of Activated T Cells [NF-AT]
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ALX-210-533-C050   50 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1439-1455 (CD1439LLVSLQNQGNNLTGSF1455) of human NF-AT5 (nuclear factor of activated T cells 5). This sequence is 94% conserved in mouse NF-AT5.
SPECIFICITY: Recognizes human NF-AT5. Detects a band of ~170kDa by Western blot.
APPLICATION: Immunocytochemistry (1:1'000)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Note: Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells 5 (NF-AT5)
Gene Names: NFAT5
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-539 Revised 27-Oct-06
Polyclonal Antibody to XRCC1 (human)
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SYNONYMS anti-X-ray Repair Cross-complementing Group 1 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY XRCC Proteins / Related Products
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ALX-210-539-R100   100 µl 250.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human XRCC1 (X-ray repair cross-complementing group 1) fused to a His-tag.
SPECIFICITY: Recognizes human XRCC1.
APPLICATION: Immunocytochemistry (1:1’000)
Immunoprecipitation (1:200)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year when stored at +4°C.
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Product Specific Literature References
XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage: M. Masson, et al.; Mol. Cell. Biol. 18, 3563 (1998) Abstract; Full Text
XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage: N. Levy, et al.; Nucl. Acids Res. 34, 32 (2006) Full Text
Related Products
Further Categories Containing This Product:
PARP Other Products • Polyclonal Antibodies
 
 
ALX-210-540 Revised 07-Dec-06
Polyclonal Antibody to PARP-1 (BRCT Domain)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-1 (BRCT Domain) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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ALX-210-540-R100   100 µl 250.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
PURITY DETAIL: Ammonium sulfate precipitate.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human PARP-1 (BRCT domain) (aa 385-524) fused to a His-tag (Prod. No. ALX-201-255).
SPECIFICITY: Recognizes human and mouse PARP-1.
APPLICATION: Immunoprecipitation (1:200)
Western Blot  (1:8’000)
Does not work for immunocytochemistry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
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Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-541 Revised 16-Oct-08
Polyclonal Antibody to PARP-3
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-3 PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-3
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ALX-210-541-R100   100 µl 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
SOURCE/HOST: From rabbit.
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. Contains 0.05% sodium azide.
IMMUNOGEN: Recombinant human PARP-3 (poly(ADP-ribose) polymerase-3).
SPECIFICITY: Recognizes human, mouse and monkey PARP-3.
APPLICATION: Immunocytochemistry (1:200)
Immunoprecipitation (5μl for 500μg total proteins)
Western Blot  (1:200-1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-550 Revised 08-Dec-04
Polyclonal Antibody to 8-Oxoguanine-DNA glycosylase (human)
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SYNONYMS anti-OGG1 (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-210-550-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum in stabilization buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human OGG1 (8-oxoguanine-DNA glycosylase).
SPECIFICITY: Recognizes human OGG1. Detects a band of ~38kDa by Western blot.
APPLICATION: Western Blot (in highly expressing cells; 1:500-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
7,8-Dihydro-8-oxoguanine (8-oxoG, 8-hydroxyguanine) and 2,6-diamino-4-hydroxyformamidopyrimidine (Fapy) are major DNA lesions formed by reactive oxygen species and are involved in mutagenic and/or lethal events in cells. 8-Oxoguanine DNA glycoxylase (OGG1) can recognize both lesions end excise the altered base, leaving behind an abasic site that is restored to guanine by the cellular abasic site repair/replication machinery. Loss of OGG1 activity results in an overal G·C to T·A transversion mutation. The human OGG1 (hOGG1) gene is located on chromosome 3 (3p26), being subject to monoallelic deletion resulting in loss of heterozygosity. This state can be found in 100% of samll-cell lung cancers and up to 50% in other form of cancer. hOGG1 exists in two main splice variants, α-hOGG1 located in the nucleus and β-hOGG1 located in the mitochondrion. OGG1 was cloned initially from S. cerevisiae and recently from human as hOGG1 (hOGH1, hMMH).
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-551 Revised 23-Feb-05
Polyclonal Antibody to O6-Methylguanine-DNA Methyltransferase
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SYNONYMS anti-O6-Methylguanine-DNA Methyltransferase PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-551-R100   100 µl 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Contains 0.05% sodium azide.
IMMUNOGEN: Human MGMT (O6-methylguanine-DNA methyltransferase).
SPECIFICITY: Recognizes human, mouse and rat MGMT.
APPLICATION: Western Blot (1:100-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Specific Literature References
O6-methylguanine-DNA methyltransferase activity in breast and brain tumors: I. Preuss, et al.; Int. J. Cancer 61, 321 (1995) Abstract
General Information
O6-Methylguanine-DNA methyltransferase (MGMT) removes O6-methylguanine from DNA by transferring the O6-alkyl group to a specific cysteine residue within its own sequence irreversibly inactivating MGMT. Unrepaired alkylated guanine will cause G·C -> A·T transition mutations by anomalous base pairing during DNA replication. DNA damage by O6-methylguanine can induce apoptosis regulated by Bcl-2 and caspases- 3 & -9. MGMT is able to remove O4-alkylated-thymine.
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products • Polyclonal Antibodies
 
 
ALX-210-552 Revised 31-Oct-06
Polyclonal Antibody to Apurinic/Apyrimidinic Endonuclease
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SYNONYMS anti-APE1 PAb
anti-APEX PAb
anti-HAP1 PAb
anti-Ref-1 PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-210-552-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Purified. Contains 0.05% sodium azide.
IMMUNOGEN: Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY: Recognizes human, mouse and rat APE1. Detects a band of ~37kDa by Western blot.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5' of an AP site to produce a hydroxyl group at the 3' terminus of an unmodified nucleotide upstream of the nick and a 5'-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5'-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3'-5' DNA exonuclease activity, a 3'-phosphodiesterase activity, a 3'-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
Product Specific Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation: S. Grosch, et al.; Cancer Res. 58, 4410 (1998) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-553 Revised 14-Oct-08
Polyclonal Antibody to DNA Polymerase β
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SYNONYMS anti-Pol β PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-210-553-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. 100µl of antiserum in stabilization buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human Pol β (DNA polymerase β).
SPECIFICITY: Recognizes human and mouse Pol β. Detects a band of  ~45kDa by Western blot.
APPLICATION: Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
During base excision repair (BER) DNA polymerase β (Pol β) is recruited by APE1. Pol β has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol β also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol β plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression o