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DNA Regulation / Transcription
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ALX-804-211 Revised 06-Feb-08
Monoclonal Antibody to PARP-1 (F1-23)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-1 MAb (F1-23)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-211-R050   50 µl 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: F1-23
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites fluid containing 0.02% sodium azide.
IMMUNOGEN: Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).
SPECIFICITY: Recognizes human and bovine PARP-1. The epitope is in the zinc finger II DNA binding domain of PARP-1. Detects bands of ~116kDa (intact PARP-1) and ~24kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with mouse or rat PARP-1.
APPLICATION: ELISA:  >1:200’000. Dilution buffer: PBS, pH 7.4, 1% non fat dried milk, 0.05% Tween 20. Detectable antigen range: 50ng of PARP-1
Immunocytochemistry: 1:500. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk.
Immunoprecipitation: 1:50. Dilution buffer: RiPA buffer Protein G-Sepharose
Western Blot: 1:5’000. Dilution buffer: PBS, pH 7.4, 5% non fat dried milk, 0.1% Tween 20. See also separate Western blot protocol. 
Functional Application: Neutralizes the biological activity of PARP (~80%).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988) Abstract
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993) Abstract
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994) Abstract
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996) Abstract; Full Text
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997) Abstract
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003) Abstract
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products • Monoclonal Antibodies
 
 
ALX-804-639 Revised 21-Aug-08
Monoclonal Antibody to PARP-2 (4G8)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-2 MAb (4G8)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-2
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ALX-804-639-L001   1 ml 250.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE: 4G8
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Tissue culture supernatant containing 0.1% sodium azide.
IMMUNOGEN: Recombinant mouse PARP-2 (poly(ADP-ribose) polymerase-2).
SPECIFICITY: Recognizes an epitope in domain E of human and mouse PARP-2. Detects a band of ~62kDa by Western blot.
APPLICATION: ELISA
Western Blot (1:50)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Anti-Poly-ADP-Ribose Polymerase-2 (PARP-2) Mouse MAb 4G8: Y. Monreal, et al.; Hybridoma 25, 102 (2006)
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery: T. Guastafierro, et al.; J. Biol. Chem. 283, 21873 (2008) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-466 Revised 31-Oct-06
Monoclonal Antibody to PARP-3 (LA6B10)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-3 MAb (LA6B10)
anti-Adprt3 MAb (LA6B10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-3
Ordering Information
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ALX-804-466-R100   100 µl 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: LA6B10
ISOTYPE: Mouse IgM
FORMULATION: Liquid. Hybridoma supernatant containing 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to N-terminal aa 8-22 (M8APKPKPWVQTEGPE22) of human PARP-3 (poly(ADP-ribose) polymerase-3).
SPECIFICITY: Recognizes human and mouse PARP-3.
APPLICATION: Immunocytochemistry (1:100).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
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Product Specific Literature References
[1] PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003) Abstract
[2] Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function: P. Chang, et al.; Nat. Cell Biol. 7, 1133 (2005) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-626 Revised 07-Apr-08
Monoclonal Antibody to PARP-10 (human) (5H11)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-10 (human) (5H11) MAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-10
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ALX-804-626-L001   1 ml 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 5H11
ISOTYPE: Rat IgG1
FORMULATION: Liquid. Tissue culture supernatant (TCS) containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human PARP-10 (poly(ADP-ribose) polymerase-10) (aa 1-907).
SPECIFICITY: Recognizes human PARP-10.
APPLICATION: Immunocytochemistry (1:10)
Immunoprecipitation (1:10)
Western Blot (1:50)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation: M. Yu, et al.; Oncogene 24, 1982 (2005) Abstract
General Literature References
Overlap of the gene encoding the novel poly(ADP-ribose) polymerase Parp10 with the plectin 1 gene and common use of exon sequences: K. Lesniewicz, et al.; Genomics 86, 38 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-220 Revised 20-Oct-08
Monoclonal Antibody to Poly(ADP-ribose) (10H)
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SYNONYMS anti-PAR MAb (10H)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Poly(ADP-ribose) [PAR]
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ALX-804-220-R100   100 µl 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: 10H
ISOTYPE: Mouse IgG3
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified from supernatant.
FORMULATION: Liquid. In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Purified poly(ADP-ribose).
SPECIFICITY: Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.
APPLICATION: Flow Cytometry
Immunocytochemistry (5-20µg/ml)
Immunohistochemistry (paraffin sections): Dilution buffer: 5% milk (non fat dried milk) in PBS to a final concentration of 5-20µg/ml).
Western Blot: Incubate 2.5µg/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Description
The monoclonal antibody 10H is directed against poly(ADP-ribose) (PAR) [1]. PAR is synthesized after activation of the nuclear DNA repair enzyme poly(ADP-ribose)polymerase (PARP) [2-5]. PARP is selectively activated by DNA strand breaks to catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, most notably PARP itself.

The amount of PAR formed in living cells with DNA damage is commensurate with the extent of the damage [3]. Under DNA damage conditions, PAR undergoes a rapid turnover, with a half-life in the range of minutes, as PAR is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP-ribose)glycohydrolase (PARG). After massive DNA damage (e.g. γ-irradiation or oxidative stress) [13-16] PAR is detectable in the first 10 minutes and disappears later on. In keratinocytes MAb 10H has been shown to detect UVB-induced apoptosis as early as 4 hour after irradiation, thus being superior to DNA laddering and the TUNEL assay [12].

Due to the very large number of endonuclease-mediated DNA breaks in apoptosis, PARP becomes strongly activated during the so-called execution phase. In the case of DNA damage-induced apoptosis, this represents a "second round" of PAR synthesis. PAR synthesized during apoptosis appears to be remarkably stable. PAR immunofluorescence appears at least as early during apoptosis as does the specific cleavage of PARP by caspase-3. As shown by several groups, this PAR immunofluorescence correlates well with other markers of apoptosis [6-12].

MAb to Poly(ADP-ribose) (10H) can be used in flow cytometry [21].
A quantitative non-isotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity using MAb to Poly(ADP-ribose) (10H) has been described [17-19].
Product Specific Literature References
[1] Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures: H. Kawamitsu, et al.; Biochemistry 23, 3771 (1984), (Original Reference) Abstract
[2] Poly(ADP-ribose) polymerase and aging: A. Bürkle; Exp. Gerontol. 33, 519 (1998), (General Reference) Abstract
[3] Increased poly(ADP-ribosyl)ation in intact cells by cisplatin treatment: A. Bürkle, et al.; Carcinogenesis 14, 559 (1993), (General Reference) Abstract
[4] Inactivation of the poly(ADP-ribose) polymerase gene affects oxygen radical and nitric oxide toxicity in islet cells: B. Heller, et al.; J. Biol. Chem. 270, 11176 (1995), (General Reference) Abstract; Full Text
[5] Detection of poly(ADP-ribose) polymerase and its reaction product poly(ADP-ribose) by immunocytochemistry: J.H. Küpper, et al.; Histochem. J. 28, 391 (1996), (General Reference) Abstract
[6] Multiparametric staining to identify apoptotic human cells: C. Negri, et al.; Exp. Cell Res. 234, 174 (1997), (Detection of Apoptosis) Abstract
[7] Poly(ADP-ribose) synthesis: a useful parameter for identifying apoptotic cells: M. Donzelli, et al.; Histochem. J. 29, 831 (1997), (Detection of Apoptosis) Abstract
[8] Poly(ADP-ribose) immunostaining to detect apoptosis induced by a neurotoxic fragment of prion protein: A. Bürkle, et al.; Histochem. J. 31, 711 (1999), (Detection of Apoptosis) Abstract
[9] Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP- ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells: R. Alvarez-Gonzalez, et al.; J. Biol. Chem. 274, 32122 (1999), (Detection of Apoptosis) Abstract; Full Text
[10] Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?: C. Soldani, et al.; Exp. Cell Res. 269, 193 (2001), (Detection of Apoptosis) Abstract
[11] Activation and Caspase-mediated Inhibition of PARP: A Molecular Switch between Fibroblast Necrosis and Apoptosis in Death Receptor Signaling: M. Los, et al.; Mol. Biol. Cell. 13, 978 (2002), (Detection of Apoptosis) Abstract; Full Text
[12] Detection of poly(ADP-ribose) by immunocytochemistry: a sensitive new method for the early identification of UVB- and H2O2-induced apoptosis in keratinocytes: H. Chang, et al.; Biol. Chem. 383, 703 (2002), (Detection of Apoptosis) Abstract
[13] Reactive oxygen species participate in mdr1b mRNA and P-glycoprotein overexpression in primary rat hepatocyte cultures: C. Ziemann, et al.; Carcinogenesis 20, 407 (1999) Abstract; Full Text
[14] Detection of poly(ADP-ribose) synthesis in Drosophila testes upon gamma-irradiation: S. Lankenau, et al.; Chromosoma 108, 44 (1999), (Detection of DNA-Damage) Abstract
[15] trans-dominant inhibition of poly(ADP-ribosyl)ation sensitizes cells against g-irradiation and N-methyl-N'-nitro-N-nitrosoguanidine but does not limit DNA replication of a polyomavirus replicon: J.H. Küpper, et al.; Mol. Cell. Biol. 15, 3154 (1995), (Detection of DNA-Damage) Abstract; Full Text
[16] 4-Amino-1,8-naphthalimide: a novel inhibitor of poly(ADP-ribose) polymerase and radiation sensitizer: A. Schlicker, et al.; Int. J. Radiat. Biol. 75, 91 (1999), (Detection of DNA-Damage) Abstract
[17] Quantitative nonisotopic immuno-dot-blot method for the assessment of cellular poly(ADP-ribosyl)ation capacity: R. Pfeiffer, et al.; Anal. Biochem. 275, 118 (1999), (Immuno-Dot-Blot Detection) Abstract
[18] Overexpression of dominant negative PARP interferes with tumor formation of HeLa cells in nude mice: evidence for increased tumor cell apoptosis in vivo: M.A. Hans, et al.; Oncogene 18, 7010 (1999) Abstract
[19] Poly(ADP-ribosyl)ation, genomic instability, and longevity: A. Bürkle; Ann. N Y Acad. Sci. 908, 126 (2000) Abstract
[20] Protection against hemorrhagic shock in mice genetically deficient in poly(ADP-ribose)polymerase: L. Liaudet, et al.; PNAS 97, 10203 (2000), (Immunohistochemistry) Abstract; Full Text
[21] Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction: G. Cipriani, et al.; J. Biol. Chem. 280, 17227 (2005) Abstract; Full Text
[22] Flow-cytometric assessment of cellular poly(ADP-ribosyl)ation capacity in peripheral blood lymphocytes: A. Kunzmann, et al.; Immun. Ageing 3, 8 (2006), (Flow Cytometry) Abstract
[23] Critical role of inducible nitric oxide synthase in degeneration of retinal capillaries in mice with streptozotocin-induced diabetes: L. Zheng, et al.; Diabetologia 50, 1987 (2007) Abstract
Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation: H. Kleine, et al.; Mol. Cell 32, 57 (2008) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-255 Revised 16-Jun-08
Monoclonal Antibody to PPARα (3B6/PPAR)
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SYNONYMS anti-Peroxisome Proliferator Activated Receptor α MAb (3B6/PPAR)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PPAR Antibodies
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ALX-804-255-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 3B6/PPAR
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml
PURITY DETAIL: Purified IgG2b.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified recombinant PPARα (peroxisome proliferator activated receptor α).
SPECIFICITY: Recognizes human, mouse and rat PPARα. Detects a band of ~52 kDa by Western blot.
APPLICATION: Gel Shift Assay
Immunoprecipitation
Western Blot (2-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Evidence that PPARalpha is complexed with the 90 kDa heat shock protein and the hepatitis virus B X-associated protein 2: W.K. Sumanasekera, et al.; J. Biol. Chem. 278, 4467 (2003) Abstract; Full Text
PPARalpha activators inhibit vascular endothelial growth factor receptor-2 expression by repressing Sp-1-dependent DNA binding and transactivation: M. Meissner, et al.; Circ. Res. 94, 324 (2004) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-662 Revised 15-Apr-08
Monoclonal Antibody to Progerin (human) (13A4)
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SYNONYMS anti-Δ50 Lamin A (human) MAb (13A4)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Intermediate Filaments Other Products
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ALX-804-662-R200   200 µl 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 13A4
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Purified supernatant containing 0.02% sodium azide.
IMMUNOGEN: Synthetic KLH-coupled peptide corresponding to aa 604-611 (G604AQSPQNC611) at the C-terminus of human progerin.
SPECIFICITY: Recognizes human progerin. Does not cross-react with endogenous prelamin A.
APPLICATION: Immunocytochemistry (1:10)
Immunoprecipitation (20µl/assay)
Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q6UYC3: human Progerin
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • Nuclear Envelope & Nuclear Transport
 
 
ALX-803-010 Revised 02-Oct-08
Monoclonal Antibody to Progesterone Receptor (αPR6)
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SYNONYMS anti-PR MAb (αPR6)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Progesterone & Progesterone Receptor / Related Products
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ALX-803-010-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
Others
CLONE: αPR6
ISOTYPE: Mouse IgG2a
PURITY DETAIL: Purified IgG2a from ascites.
FORMULATION: Lyophilized. Contains 0.05% sodium azide.
RECONSTITUTION: Reconstitute with PBS.
IMMUNOGEN: Purified chicken PR (progesterone receptor).
SPECIFICITY: Recognizes the B form of human, mouse, rat, sheep, rabbit, bovine, chicken and guinea pig PR. Detects a band of ~110kDa in chicken and rabbit, and a doublet or triplet band of ~120kDa in human by Western blot. Does not cross-react with ER (estrogen receptor) or GR (glucocorticoid receptor).
APPLICATION: Immunocytochemistry
Immunohistochemistry (frozen sections (20µg/ml), paraffin sections (5µg/ml))
Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Ovine uterine gland knock-out model: effects of gland ablation on the estrous cycle: C. Allison Gray, et al.; Biol. Reprod. 62, 448 (2000) Abstract; Full Text
General Information
Avian and mammalian PR isolated from tissue soluble extracts can exist in one of two different molecular forms A and B. Human PR consists of a 94kDa A form and a 120kDa B form. Chicken PR consists of a 78kDa A form and a 110kDa B form. Alpha PR6 reacts with both the 120kDa B form of human PR and the 110kDa B form of chicken PR.
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers
 
 
ALX-803-011 Revised 11-Nov-05
Monoclonal Antibody to Progesterone Receptor (chicken) (αPR22)
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SYNONYMS anti-PR (chicken) MAb (αPR22)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Progesterone & Progesterone Receptor / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-011-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: αPR22
ISOTYPE: Mouse IgG2b
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute in PBS.
IMMUNOGEN: Purified chicken PR (progesterone receptor).
SPECIFICITY: Recognizes chicken PR. Does not cross-react with chicken ER (estrogen receptor) or GR (glucocorticoid receptor).
APPLICATION: Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers
 
 
ALX-803-043 Revised 11-Nov-05
Monoclonal Antibody to Progesterone Receptor (human) (phosphorylated) (pSer190) (1154)