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DNA Intercalators & Crosslinkers

DNA Replication

Nucleotides / Related Products

Topoisomerases / Related Products

ALX-801-091

Revised 29-Sep-08

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PC10)
SYNONYMS	anti-PCNA MAb (PC10)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Proliferating Cell Nuclear Antigen [PCNA] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-801-091-L001		1 ml	480.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Goat Sheep Dog Pig Monkey Others
CLONE:	PC10
ISOTYPE:	Mouse IgG2a
FORMULATION:	Liquid. In 0.01M PBS containing 0.1% sodium azide and 1% BSA.
IMMUNOGEN:	Recombinant rat PCNA (proliferating cell nuclear antigen).
SPECIFICITY:	Recognizes PCNA from all vertebrate species, insects and S. pombe. Intense nuclear staining of proliferating cells in normal and tumor tissues.
APPLICATION:	Immunohistochemistry (paraffin sections): Can be used for formalin-fixed, paraffin-embedded tissues. Pretreatment is not required. Recommended dilution is ≥1:40 for biotin based detection. For optimal staining, incubate 20-60 min. at room temperature. Optimal conditions must be determined individually.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

Proliferating Cell Nuclear Antigen (PCNA) is associated with the DNA replication fork and its major function is described as a processivity factor for replicative DNA polymerases (polymerases δ and ε ) acting as a sliding clamp. It has a highly conserved amino acid sequence and has been identified in all eukaryotes and is being used as a proliferating marker. PCNA is also able to interact with multiple partners involved in Okazaki fragment joining, DNA repair, DNA methylation and chromatin assembly. In DNA repair PCNA has been reported not only for nucleotide excision repair (NER) (in concert with XPG and ERCC1), but also for base excision repair (BER) (binding to uracil-DNA glycosylase) and mismatch repair (MMR) (binding to mismatch repair enzymes MSH2 & MLH1). It is further associated with cell cycle control through cdk-cyclins during G1 to S phase.

Further Categories Containing This Product:

Monoclonal Antibodies • Tumor Markers

ALX-804-229

Revised 11-Jan-07

Monoclonal Antibody to Rad9 (human) (93A535)
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	DNA Damage Checkpoint Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-229-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	93A535
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:	Recombinant human Rad9.
SPECIFICITY:	Recognizes human Rad9. Detects a band of ~50kDa by Western blot.
APPLICATION:	Immunocytochemistry (see [7]) Immunoprecipitation Western Blot (1-2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.

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Product Specific Literature References

[1] Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage: R.H. Schiestl, et al.; Mol. Cell. Biol. 9, 1882 (1989) Abstract
[2] Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage: T.A. Weinert & L.H. Hartwell; Mol. Cell. Biol. 10, 6554 (1990) Abstract
[3] A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene: H.B. Lieberman, et al.; PNAS 93, 13890 (1996) Abstract
[4] MEC1-dependent phosphorylation of Rad9p in response to DNA damage: A. Emili; Mol. Cell 2, 183 (1998) Abstract
[5] Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex: E. Volkmer & L.M. Karnitz; J. Biol. Chem. 274, 567 (1999) Abstract; Full Text
[6] The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1: R.P. St. Onge, et al.; Mol. Biol. Cell 10, 1985 (1999) Abstract
[7] Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-x L and promotes apoptosis: K. Komatsu, et al.; Nature Cell Biol. 2, 1 (2000) Abstract

General Information

Rad9 is a cell cycle checkpoint protein: DNA damage leads to extensive phosphorylation of Rad9 [4] which then inhibits progression from G2 in order to preserve cell viability, allowing proper ligation of DNA breaks before the entry of cells into mitosis [1,2]. The human homolog [3] of S. pombe Rad9 is physically proximal to the upstream (DNA damage) signal and associates with two other checkpoint control proteins, Rad1 and HHUS1 [5,6]. Rad9 also interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Overexpression of Rad9 in mammalian cells leads to apoptosis, where as antisense Rad9 RNA suppresses cell death induced by methyl methanesulphonate [7].

Further Categories Containing This Product:

DNA Replication Other Products • Monoclonal Antibodies

ALX-804-624

Revised 20-Jun-05

Monoclonal Antibody to Doxorubicin (MAD11)
SYNONYMS	anti-DXR MAb (MAD11) anti-14-Hydroxydaunomycin MAb (MAD11) anti-Adriamycin MAb (MAD11)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-624-C100		100 µg	320.00 USD

Product Specification

CLONE:	MAD11
ISOTYPE:	Mouse IgG2
CONCENTRATION:
FORMULATION:	Liquid. Purified antibody containing 150mM sodium chloride and 0.02% sodium azide, pH 7.4.
IMMUNOGEN:	Doxorubicin-BSA.
SPECIFICITY:	Recognizes anthracycline compounds including doxorubicin analogs and derivatives.
APPLICATION:	ELISA Immunohistochemistry Functional Application: In vivo antidotic effect.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Do not freeze.

Product Specific Literature References

Monoclonal antibodies against doxorubicin: A. Balsari, et al.; Int. J. Cancer 42, 798 (1988) Abstract
A new monoclonal antibody recognizing anthracyclinic molecule: A. Balsari, et al.; Anticancer Res. 10, 129 (1990) Abstract
Anti-drug monoclonal antibodies antagonize toxic effect more than anti-tumor activity of doxorubicin: A. Balsari, et al.; Int. J. Cancer 47, 889 (1991) Abstract
An anti-doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug: A. Sardini, et al.; Int. J. Cancer 50, 617 (1992) Abstract
Protection against doxorubicin-induced alopecia in rats by liposome-entrapped monoclonal antibodies: A.L. Balsari, et al.; FASEB J. 8, 226 (1994) Abstract
Effect of a bifunctional monoclonal antibody directed against a tumor marker and doxorubicin on the growth of epidermoid vulvar carcinoma grafted in athymic mice: D. Morelli, et al.; Cell Biophys. 24-25, 119 (1994) Abstract
Relevance of antibody valency in EGF receptor modulation: D. Morelli, et al.; Scand. J. Immunol. 39, 453 (1994) Abstract
Oral administration of anti-doxorubicin monoclonal antibody prevents chemotherapy-induced gastrointestinal toxicity in mice: D. Morelli, et al.; Cancer Res. 56, 2082 (1996) Abstract
Apoptosis of hair follicle cells during doxorubicin-induced alopecia in rats: R. Cece, et al.; Lab. Invest. 75, 601 (1996) Abstract
Intratibial injection of an anti-doxorubicin monoclonal antibody prevents drug-induced myelotoxicity in mice: D. Morelli, et al.; Br. J. Cancer 75, 656 (1997) Abstract
Topical administration of a doxorubicin-specific monoclonal antibody prevents drug-induced mouth apoptosis in mice: A. Balsari, et al.; Br. J. Cancer 85, 1964 (2001) Abstract
Role of proliferation in HER2 status predicted response to doxorubicin: M. Campiglio, et al.; Int. J. Cancer 105, 568 (2003) Abstract

Further Categories Containing This Product:

Cancer Other Products • Topoisomerases Other Products • Antibiotics - Topoisomerase Inhibitors • Monoclonal Antibodies

ALX-850-013

Revised 24-Feb-05