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Poly(ADP-ribose) Polymerase [PARP] / Related Products
You are here: Product Lines > DNA Regulation / Transcription > Poly(ADP-ribose) Polymerase [PARP] / Related Products
 
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AG-N0812 Revised 23-Oct-08 New product
Nampt/Visfatin (mouse) (IntraCellular) ELISA Kit
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse) (IntraCellular) ELISA Kit
PBEF1 (mouse) (IntraCellular) ELISA Kit
Pre-B-Cell Colony Enhancing Factor 1 (mouse) (IntraCellular) ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-N0812EK-KI01   96 wells 640.00 USD Add To Cart
AG-N0812TP-KI01 Twin Plex 2x96 wells 940.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SENSITIVITY: 50pg/ml (range 0 to 64ng/ml)
SPECIFICITY: Does not cross-react with human or rat Nampt/visfatin.
APPLICATION: For the quantitative determination of Nampt/visfatin in mouse cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
NAD+ Metabolism / Related Products • ELISA & EIA Kits
 
 
ALX-460-008 Revised 02-Jun-06
Nicotinamide
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SYNONYMS Niacinamide
3-Pyridinecarboxylic amide
Vitamin PP
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-460-008-G010   10 g 10.00 USD Add To Cart
Product Specification
FORMULA: C6H6N2O
MW: 122.1
CAS NUMBER: 98-92-0
MERCK INDEX: 14: 6523
PURITY: ≥99%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water; also soluble in 100 % ethanol or glycerol; slightly soluble in chloroform; almost insoluble in ether or acetone.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
USE/STABILITY: Susceptible to hydrolysis in acidic or alkaline solution.
HANDLING: Protect from light.
HAZARD: IRRITANT. MAY BE MUTAGENIC.

Further Categories Containing This Product:
Vitamins • DNA Repair Other Products • PARP Inhibitors
 
 
ALX-460-009 Revised 08-Jan-03
Nicotinic acid
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SYNONYMS Niacin
3-Pyridinecarboxylic acid
Vitamin B
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-460-009-G010   10 g 13.00 USD Add To Cart
Product Specification
FORMULA: C6H5NO2
MW: 123.1
CAS NUMBER: 59-67-6
MERCK INDEX: 14: 6525
PURITY: ≥98% (HPLC)
SOLUBILITY: Soluble in water or 96% ethanol (1g/100ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C

Further Categories Containing This Product:
Vitamins
 
 
ALX-201-237 Revised 12-Aug-08
NMNAT1 (human) (recombinant) (His)
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SYNONYMS NMN Adenylyltransferase 1 (human) (recombinant) (His)
Nicotinamide Mononucleotide Adenylyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-237-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 33.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT1 is fused at the N-terminus to a His-tag.
CONCENTRATION:

~1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
SPECIFIC ACTIVITY: 10-20U/mg
APPLICATION: Well suited for the synthesis of NAD due to high specific activity (10-20U/mg) and high substrate selectivity compared to NMNAT3 (human) (recombinant) (Prod. No. ALX-201-238).
Note: When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min. before the assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C. Unstable in solutions containing less than 250mM salt.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem. 276, 406 (2001) Abstract; Full Text
Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett. 492, 95 (2001) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9HAN9: NMNAT1 (human)
Related Products
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-429 Revised 18-Sep-08 New product
NMNAT1 (human) (recombinant) (His)
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SYNONYMS NMN Adenylyltransferase 1 (human) (recombinant) (His)
Nicotinamide Mononucleotide Adenylyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-429-C010   10 µg 140.00 USD Add To Cart
ALX-201-429-C050   50 µg 280.00 USD Add To Cart
Product Specification
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. The mature peptide of human NMNAT1 (aa 2-279) is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.25mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in 55mM TRIS, pH 8.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/μg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9HAN9: NMNAT1 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-238 Revised 18-Mar-08
NMNAT3 (human) (recombinant)
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SYNONYMS NMN Adenylyltransferase 3 (human) (recombinant)
Nicotinamide Mononucleotide Adenylyltransferase 3 (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-238-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 30.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT3 is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.8mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
APPLICATION: Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1 (human) (recombinant) (Prod. No. ALX-201-237).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis: X. Zhang, et al.; J. Biol. Chem. 278, 13503 (2003) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96T66: NMNAT3 (human)
Related Products
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-270-370 Revised 01-Jul-08
NU1025
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SYNONYMS 8-Hydroxy-2-methylquinazoline-4-one
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP Inhibitors
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-370-M001   1 mg 30.00 USD Add To Cart
ALX-270-370-M005   5 mg 90.00 USD Add To Cart
ALX-270-370-M025   25 mg 290.00 USD Add To Cart
Product Specification
FORMULA: C9H8N2O2
MW: 176.2
CAS NUMBER: 90417-38-2
PURITY: ≥98%
FORMULATION: Off-white to purple solid.
SOLUBILITY: Soluble in DMSO (25mg/ml). This solution can be further diluted 1:100 in tissue culture medium before use.
RECONSTITUTION: Reconstitute with DMSO.
SHIPPING: AMBIENT
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Packaged under inert gas. Protect from light.

Product Description
A potent poly(ADP-ribose) polymerase 1 (PARP-1) inhibitor (IC50=400nM) that potentiates the cytotoxicity of various DNA-active agents, including the DNA-methylating compound MTIC, the DNA strand break-inducing drug temozolomide (Prod. No. ALX-420-044), topotecan (Prod. No. ALX-350-133), bleomycin (Prod. No. ALX-630-107), and ionizing radiation effective in murine L1210 leukemia cells, CHO, and in a variety of human tumor cell lines.
Product Specific Literature References
Potentiation of temozolomide-induced cytotoxicity: a comparative study of the biological effects of poly(ADP-ribose) polymerase inhibitors: S. Boulton, et al.; Br. J. Cancer 72, 849 (1995) Abstract
Nephron-sparing surgery for renal cell carcinoma: J.H. Griffin & R.C. Flanigan; Tech. Urol.
Potentiation of anti-cancer agent cytotoxicity by the potent poly(ADP-ribose) polymerase inhibitors NU1025 and NU1064: K.J. Bowman, et al.; Br. J. Cancer 78, 1269 (1998) Abstract
Resistance-modifying agents. 5. Synthesis and biological properties of quinazolinone inhibitors of the DNA repair enzyme poly(ADP-ribose) polymerase (PARP): R.J. Griffin, et al.; J. Med. Chem. 41, 5247 (1998) Abstract
Interactive effects of inhibitors of poly(ADP-ribose) polymerase and DNA-dependent protein kinase on cellular responses to DNA damage: S. Boulton, et al.; Carcinogenesis 20, 199 (1999) Abstract; Full Text
Potentiation of temozolomide and topotecan growth inhibition and cytotoxicity by novel poly(adenosine diphosphoribose) polymerase inhibitors in a panel of human tumor cell lines: C.A. Delaney, et al.; Clin. Cancer Res. 6, 2860 (2000) Abstract; Full Text
Further Categories Containing This Product:
DNA Repair Other Products
 
 
ALX-202-045 Revised 19-Jul-06
PARG (bovine)
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SYNONYMS Poly(ADP-ribose) glycohydrolase (bovine)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARG / Related Products
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ALX-202-045-UC01   0.1 U 375.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from calf thymus.
QUANTITY: 100µl
CONCENTRATION:     
PURITY: Purified by substrate chromatography. The enzyme is composed of two major bands at 74kDa and 59kDa
SPECIFIC ACTIVITY: ~10’000U/mg protein. 1U is defined as the amount of enzyme that hydrolyzes 1nmol of poly(ADP-ribose) per min. at 37°C.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
An affinity matrix for the purification of poly(ADP-ribose) glycohydrolase: H. Thomassin, et al.; Nucl. Acid Res. 18, 4691 (1990) Abstract
Poly(ADP-ribosyl)ation of chromatin in an in vitro poly(ADP-ribose)-turnover system: H. Thomassin, et al.; Biochim. Biophys. Acta 1137, 171 (1992) Abstract
Mode of action of poly(ADP-ribose) glycohydrolase: G. Brochu, et al.; Biochim. Biophys. Acta 1219, 342 (1994) Abstract
General Information
For further dilution use the following enzyme dilution buffer: 50mM KPO4, pH 7.5, 50mM KCl, 10 mM β-mercaptoethanol, 10% glycerol, 1mM DTT, 0.1% Triton.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O02776: Poly(ADP-ribose) glycohydrolase (bovine)
Further Categories Containing This Product:
Enzymes • Natural Proteins
 
 
ALX-202-042 Revised 16-Apr-08
PARP-1 (bovine) (~90%)
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SYNONYMS Poly(ADP-ribose) Polymerase-1 (bovine) (~90%)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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ALX-202-042-C010   10 µg 180.00 USD Add To Cart
Product Specification
EC: 2.4.2.30
SOURCE/HOST: Isolated from calf thymus.
CONCENTRATION: 335μg/ml
PURITY: ~90% (SDS-PAGE, Western blot)
FORMULATION: Liquid. In 60mM kalium phosphate, pH 7.2, containing 0.5 - 2M kalium chloride, 10% glycerol, 1mM DTT, 10mM β−mercaptoethanol, 0.1mM 0.1 PMSF.
SPECIFIC ACTIVITY: 500-700U/mg protein.
One unit is defined as the amount of enzyme that synthesizes 1nmol poly(ADP-ribose) per min. at 25°C.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Do not freeze/thaw.
Product Specific Literature References
Erasable blot of poly(ADP-ribose) polymerase: S. Desnoyers, et al.; Anal. Biochem. 218, 470 (1994) Abstract
Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA: S. Muller, et al.; Clin. Immunol. Immunopathol. 73, 187 (1994) Abstract
Characterization of the execution phase of apoptosis in vitro using extracts from condemned-phase cells: Y.A. Lazebnik, et al.; J. Cell Sci. Suppl. 19, 41 (1995) Abstract
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah, et al.; Anal. Biochem. 227, 1 (1995), (Review) Abstract
Detection of poly(ADP-ribose) polymerase and its apoptosis-specific fragment by a nonisotopic activity-western blot technique: G.M. Shah, et al.; Anal. Biochem. 232, 251 (1995) Abstract
C.J. Froelich, et al.; BBRC 227, 658 (1996) Abstract
Proteolysis of poly(ADP-ribose) polymerase by caspase 3: kinetics of cleavage of mono(ADP-ribosyl)ated and DNA-bound substrates: D. D'Amours, et al.; Radiat. Res. 150, 3 (1998) Abstract
Towards new neuroprotective agents: design and synthesis of 4H-thieno[2,3-c] isoquinolin-5-one derivatives as potent PARP-1 inhibitors: R. Pellicciari, et al.; Farmaco 58, 851 (2003) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P18493: PARP-1 (bovine)
Further Categories Containing This Product:
Enzymes • Natural Proteins
 
 
ALX-202-046 Revised 25-Oct-06
PARP-1 (bovine) (~98%)
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SYNONYMS Poly(ADP-ribose) Polymerase-1 (bovine) (~98%)