ALEXIS Biochemicals: DNase I-like Endonuclease 3 (human) (recombinant)

ALX-201-077

Revised 10-Jul-07

DNase I-like Endonuclease 3 (human) (recombinant)
SYNONYMS	DNAS1L3 (human) (recombinant) Deoxyribonuclease γ (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Endonucleases/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-077-C010		10 µg	350.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM potassium chloride and 50% glycerol.
APPLICATION:	For studying DNAS1L3 catalyzed oligonucleosomal DNA fragmentation.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Specific Literature References

Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments: A.G. Yakovlev, et al.; Nucl. Acids Res. 27, 1999 (1999) Abstract
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase: A.G. Yakovlev, et al.; J. Biol. Chem. 275, 21302 (2000) Abstract; Full Text
Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation: A.H. Boulares, et al.; J. Biol. Chem. 277, 372 (2002) Abstract; Full Text
The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells: A.H. Boulares, et al.; Cancer Res. 62, 4439 (2002) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

For maximum activity, use at pH 7.5 with 2.5mM Ca²⁺ and 5mM Mg²⁺. Causes single strand nicks in duplex DNA and can be inhibited by Zn²⁺, but not by actin.

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