ALEXIS Biochemicals: Indirubin

ALX-270-361

Revised 28-Sep-07

Indirubin
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	CDK & Cyclin Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-361-M001		1 mg	50.00 USD
ALX-270-361-M005		5 mg	200.00 USD

Product Specification

FORMULA:	C₁₆H₁₀N₂O₂
MW:	262.3
CAS NUMBER:	479-41-4
SOURCE/HOST:	Synthetic.
PURITY:	≥98% (¹H-NMR, HPLC)
APPEARANCE:	Purple powder.
SOLUBILITY:	Soluble in DMSO (10mg/ml) or methanol (0.3mg/ml). Sparingly soluble in aqueous buffers (12.5µg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 2 years when stored at +4°C in the dark. Stock solutions should be stored in the dark and used within 1 month.
HANDLING:	Keep cool and dry. Protect from light.
IDENTITY:	Identity determined by ¹H-NMR, ¹³C-NMR, IR, ESI-MS and UV.

Product Description

Inhibitor of cyclin-dependent kinases (CDK1/cyclin B (IC₅₀=10µM), CDK2/cyclin A (IC₅₀=2.2µM), CDK2/cyclin E (IC₅₀=7.5µM), CDK4/cyclin D1 (IC₅₀=12µM), CDK5/p35 (IC₅₀=5.5µM)) [1] and of GSK-3β (IC₅₀=600nM) [2]. Inhibits inflammatory reaction in delayed-type hypersensitivity in mice [3]. Ligand of human aryl hydrocarbon receptor [4]. Exhibits anti-cancer activity [5] [6].

Product Specific Literature References

[1] Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases: R. Hoessel, et al.; Nature Cell Biol. 1, 60 (1999) Abstract
[2] Indirubins inhibit glycogen synthase kinase-3 beta and CDK5/p25, two protein kinases involved in abnormal tau phosphorylation in Alzheimer’s disease. A property common to most cyclin-dependent kinase inhibitors?: S. Leclerc, et al.; J. Biol. Chem. 276, 251 (2001) Abstract; Full Text
[3] Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity: T. Kunikata, et al.; Eur. J. Pharmacol. 410, 93 (2000) Abstract
[4] Indirubin and indigo are potent aryl hydrocarbon receptor ligands present in human urine: J. Adachi, et al.; J. Biol. Chem. 276, 31475 (2001) Abstract; Full Text
[5] Molecular mechanisms of indirubin and its derivatives: novel anticancer molecules with their origin in traditional Chinese phytomedicine: G. Eisenbrand, et al.; J. Cancer Res. Clin. Oncol. 130, 627 (2004) Abstract
[6] Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway: G. Sethi, et al.; J. Biol. Chem. 281, 23425 (2006) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Indirubin is derived from various natural sources, such as Indigofera indica, Isatis tinctoria and I. indigotica by fermentation, oxidation and the presence of light as a by product of indigo formation. Indirubin is also present in human urine and fetal bovine serum [4]. Preclinical studies of indirubin its confirmed anti-tumor activity. Efficacy against chronic myelocytic leukemia was demonstrated in clinical trials in China [2].

ALX-270-271	Indirubin-3'-monoxime
ALX-270-296	Indirubin-5-sulfonic acid . sodium salt
ALX-270-424	5-Iodo-indirubin-3'-monoxime
ALX-430-149	7BIO
ALX-430-157	Me-7BIO
ALX-430-156	6BIO