ALEXIS Biochemicals: iODN (ttaggg)4 (TLRgrade™) (synthetic) (Inhibitory for human)

ALX-746-251

Revised 06-Oct-06

iODN (ttaggg)4 (TLRgrade™) (synthetic) (Inhibitory for human)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Oligodeoxynucleotides [ODNs]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-746-251-T050		50 tests	155.00 USD

Product Specification

SEQUENCE:	5’-tttagggttagggttagggttaggg-3’ (lower case letters indicate phosphorothioate linkage).
MW:	8288 (ammonium salt)
SOURCE/HOST:	Synthetic.
QUANTITY:	24nmol (~200µg). Sufficient for ~50 cellular inhibition assays. Working concentration depends upon concentration, type and MW of stimulating human CpG ODN, the cellular system and read-out used. Titrate at 3-10-fold molar excess for inhibition.
PURITY DETAIL:	Endotoxin tested - TLRgrade™.
FORMULATION:	Lyophilized. Sterile.
ENDOTOXIN CONTENT:	<0.002EU/µg (LAL test; BioWhittaker)
RECONSTITUTION:	For a 100µM stock solution, dissolve the total vial content in 240µl endotoxin-free water (included) or PBS (to order seperately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Protect from light.

Product Description

Inhibitory iODN oligodeoxynucleotide (human) with phosphorothioate backbone. For active human CpG compounds see e.g. ODN 2216 (Prod. No. ALX-746-005).

General Information

BACKGROUND/TECHNICAL INFORMATION

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).

Related Products:

- PBS (endotoxin-free) (Prod. No. ALX-505-007)

- ODN 2216 (TLRgrade™) (synthetic) (Prod. No. ALX-746-005)

- ODN 2006 (TLRgrade™) (synthetic) (Prod. No. ALX-746-006)

- ODN 2395 (TLRgrade™) (synthetic) (Prod. No. ALX-746-020)

General Literature References

Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation: I. Gursel, et al.; J. Immunol. 171, 1393 (2003) Abstract
Suppressive oligodeoxynucleotides inhibit Th1 differentiation by blocking IFN-gamma- and IL-12-mediated signaling: H. Shirota, et al.; J. Immunol. 173, 5002 (2004) Abstract
Inhibitors of TLR-9 act on multiple cell subsets in mouse and man in vitro and prevent death in vivo from systemic inflammation: O. Duramad, et al.; J. Immunol. 174, 5193 (2005) Abstract
Suppressive oligodeoxynucleotides protect mice from lethal endotoxic shock: H. Shirota, et al.; J. Immunol. 174, 4579 (2005) Abstract
Therapeutic potential of oligonucleotides expressing immunosuppressive TTAGGG motifs: D.M. Klinman, et al.; Ann. NY Acad. Sci. 1058, 87 (2005) Abstract

ALX-746-020	ODN 2395 (TLRgrade™) (synthetic)
ALX-746-006	ODN 2006 (TLRgrade™) (synthetic)
ALX-505-007	PBS (endotoxin-free)
ALX-505-008	ddWater (endotoxin-free)
ALX-746-005	ODN 2216 (TLRgrade™) (synthetic)