ALEXIS Biochemicals: Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)

ALX-581-200

Revised 16-Sep-08

Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Lipopolysaccharides [LPS] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-581-200-L002		2 ml	320.00 USD

Product Specification

SOURCE/HOST:	Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method [1,2]), converted to the uniform salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION:	1mg/ml
PURITY:	≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
FORMULATION:	Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY:	Activates Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Keep sterile.
HAZARD:	HIGHLY PYROGENIC. HARMFUL.

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Product Specific Literature References

[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Methods in Carbohydrate Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
[4] R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006) Abstract