| SOURCE/HOST: |
Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human FLIP (aa 1-480) fused at the C-terminus to a HA-tag. |
| QUANTITY: |
100µl corresponding to 2x106 cells. |
| FORMULATION: |
Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT). |
| APPLICATION: |
Positive control in Western blot for MAb to FLIP (Dave-2) (Prod. No. ALX-804-127) and MAb to FLIP (human) (NF6) (Prod. No. ALX-804-428).
Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate. |
| SHIPPING: |
SHIPPED ON BLUE ICE |
| LONG TERM STORAGE: |
-20°C |
| HANDLING: |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
