ALEXIS Biochemicals: Ac-DEVD-AFC

ALX-260-032

Revised 27-Apr-05

Ac-DEVD-AFC
SYNONYMS	Caspase-3 Substrate (Fluorogenic)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Substrates

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-032-M001		1 mg	47.00 USD
ALX-260-032-M005		5 mg	138.00 USD

Product Specification

SEQUENCE:	Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin)
FORMULA:	C₃₀H₃₄F₃N₅O₁₃
MW:	729.6
PEPTIDE CONTENT:	65-95%
PURITY:	≥96% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Keep cool and dry.

Product Description

Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7. This product is similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp²¹⁶ for caspase-3.

Product Specific Literature References

BAX-induced cell death may not require interleukin 1 β-converting enzyme-like proteases: J. Xiang, et al.; Proc. Natl. Acad. Sci. USA 93, 14559 (1996) Abstract
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver: J.M. Chandler, et al.; J. Biol. Chem. 273, 10815 (1998) Abstract; Full Text
Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP: M. Marcelli, et al.; Cancer Res. 58, 76 (1998) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

AFC has an excitation maximum of 400nm and an emission maximum of 505nm.

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