ALEXIS Biochemicals: Monoclonal Antibody to Apurinic/Apyrimidinic Endonuclease (human) (13B 8E5C2)

ALX-804-213

Revised 14-Feb-08

Monoclonal Antibody to Apurinic/Apyrimidinic Endonuclease (human) (13B 8E5C2)
SYNONYMS	anti-APE1 (human) MAb (13B 8E5C2) anti-APEX (human) MAb (13B 8E5C2) anti-HAP1 (human) MAb (13B 8E5C2) anti-Ref-1 (human) MAb (13B 8E5C2)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Endonucleases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-213-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	13B 8E5C2
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY:	Recognizes human APE1. Detects a band of ~37kDa by Western blot.
APPLICATION:	Immunohistochemistry (paraffin sections (1:100)) Immunoprecipitation Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer: M.R. Kelley, et al.; Clin. Cancer Res. 7, 824 (2001) Abstract
Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers: D.H. Moore, et al.; Clin. Cancer Res. 6, 602 (2000) Abstract

General Information

Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κ B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.

Further Categories Containing This Product:

Monoclonal Antibodies