ALEXIS Biochemicals: Control:Fc Fusion Protein (human) (recombinant)

ALX-203-004

Revised 11-Aug-08

Control:Fc Fusion Protein (human) (recombinant)
SYNONYMS	Thy-1 (RLE) (human):Fc (human) (recombinant)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Negative Controls & Reference Sera

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-203-004-C050		50 µg	380.00 USD

Product Specification

MW:	~49kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins.
CONCENTRATION:	1mg/ml (after reconstitution)
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
RECONSTITUTION:	Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Does not bind to β3-integrin nor to any of a large number of tested TNF-superfamily members including rhsTRAIL, rhsCD40L, rhsAPRIL, etc.
APPLICATION:	Control compound for Fc Tagged Proteins, ELISA, Flow Cytometry, Functional Application (serves as a negative control in functional assays of TNF-superfamily members).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Description

Negative control for Fc chimeric proteins. Does not bind to a series of ligands in an ELISA assay. Does not inhibit binding between the thymoma cell line EL-4 expressing β3-integrin and astrocytes expressing high level of Thy-1 in mice.

Product Specific Literature References

Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005) Abstract
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment: T. Keiper, et al.; FASEB J. 19, 2078 (2005) Abstract; Full Text

General Information

Thy-1 is a glycosyl phosphatidylinositol (GPI)- anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in T-lymphocytes and cells of the neuronal system. Thy-1 is involved in cell adhesion, and the presence of a RLD motifs, which is recognized by intengrins, strengthen the hypothesis that the Thy-1:integrin interaction is responsible for cell attachment. In astrocytes, binding of Thy-1 to β3-integrin triggers tyrosine phosphorylation of focal adhesion proteins.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites: L. Leyton, et al.; Curr. Biol. 11, 1028 (2001) Abstract
Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function: A.M. Avalos, et al.; Biol. Res. 35, 231 (2002) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Epitope Tags / Related Products

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