ALEXIS Biochemicals: Caspase-8 (active) (human) (recombinant)

ALX-201-062

Revised 04-Aug-08

Caspase-8 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-062-U025		25 U	203.00 USD
ALX-201-062-U100		100 U	371.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

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General Information

Caspase-8 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q14790: Caspase-8 (human) (precursor)
AfCS Signalling Gateway link A000503: Caspase-8 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

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