ALEXIS Biochemicals: Monoclonal Antibody to STAT6 (phosphorylated) (pTyr641) (16E12)

ALX-804-272

Revised 13-Jun-08

Monoclonal Antibody to STAT6 (phosphorylated) (pTyr641) (16E12)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	STATs / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-272-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	16E12
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide corresponding to a portion of human STAT6 phophorylated at Tyr⁶⁴¹.
SPECIFICITY:	Recognizes the GRGpYVPA motif of human, mouse (weak) and rat (weak) activated STAT6 phosphorylated at Tyr⁶⁴¹. Does not cross-react with the non-phosphorylated form of STAT6 nor with unrelated tyrosine-phosphorylated proteins.
APPLICATION:	Immunohistochemistry Western Blot (1HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-018).

Product Specific Literature References

IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005) Abstract; Full Text

General Information

STATs (signal transducing activators of transcription) are mediators involved in cytokine signalling. In response to a specific cytokine signal, STATs move to the nucleus and induce transcription. STAT6 is activated primarily by IL-4 and IL-13. Upon activation, STAT6 is phosphorylated at tyrosine 641 by Janus Kinases (JAKs). Phosphorylated STAT6 forms head-to-tail heterodimers and translocates to the nucleus where it participates in transcriptional control.

Further Categories Containing This Product:

Phospho-specific Antibodies • Monoclonal Antibodies • Autophagy Other Products

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