ALEXIS Biochemicals: Polyclonal Antibody to p51B

ALX-210-769

Revised 11-Apr-06

Polyclonal Antibody to p51B
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-769-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	2mg/ml.
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 623-641(N⁶²³FDMDARRNKQQRIKEEGE⁶⁴¹) of C-terminal human p51B.
SPECIFICITY:	Recognizes human and mouse p51B. Detects a band of ~75kDA by Western blot. Does not cross-react with p51A.
APPLICATION:	Immunocytochemistry (1:300) Immunohistochemistry (paraffin sections) Western Blot (1:1'000)
QUALITY CONTROL:	Western blot on mouse lung and human K562 cell line. As expected, some of the p51B is degraded in the tumor cell line K562.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-041.

General Information

p51 is a member of the p53 tumor suppressor gene family, which is induced by DNA damage and/or stress stimuli, causing cells to undergo G1-arrest or apoptotic death. Unlike ubiquitous expression of p53, expression of p51 is restricted to a limited number of tissues. The p51 gene encodes two major splicing variants, p51A (448 aa protein with a molecular weight of 50.9kDa) and p51B (641 aa protein with a molecular weight of 71.9kDa), a human homolog of rat Ket. p51A and p51B differ in their carboxyl-terminal structure.

General Literature References

Cloning and functional analysis of human p51, which structurally and functionally resembles p53  : M. Osada, et al.; Nat. Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator  : S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation  : S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression  : P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract

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