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ALX-201-180 Revised 29-Jul-08
Guanylyl Cyclase (α1β1), Soluble (human) (recombinant) (His) (enriched)
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SYNONYMS GTP Pyrophosphate-lyase (cycling), Soluble (human) (recombinant) (His) (enriched)
sGCα1/sGCβ1 (human) (recombinant) (His) (enriched)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-180-R050   50 µl 240.00 USD Add To Cart
Product Specification
EC: 4.6.1.2
SOURCE/HOST: Produced in Sf9 cells. Fused at the C-terminus to a His-tag.
CONCENTRATION: ≥1.5mg/ml (total protein)
FORMULATION: Liquid. Enriched fraction in 50mM TEA, pH 7.4 containing 1mM EDTA/EGTA, 5mM GSH and 30% glycerol.
SPECIFIC ACTIVITY: - Basal: ≥0.001µmol/mg/min.
- 200µM DEA NONOate (Prod. No. ALX-430-034): ≥0.5µmol/mg/min.
- 1µM BAY41-2272 (Prod. No. ALX-420-030): ≥0.01µmol/mg/min.
APPLICATION: Enzymatic studies and drug screening.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: We do not recommend to store for longer periods after opening and thawing of the product.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981) Abstract
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998) Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999) Abstract
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000) Abstract; Full Text
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Prepared by one column purification.

Swiss-prot link:
Human α1 subunit-accession AAB94794, locus CYG3_human
Human β1 subunit-accession AAH47620, locus CYG1_human
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
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