ALEXIS Biochemicals: Annexin V (human) (recombinant) (APC)

ALX-209-252

Revised 26-Aug-08

Annexin V (human) (recombinant) (APC)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-252-T020		20 tests	106.00 USD
ALX-209-252-T100		100 tests	279.00 USD

Product Specification

MW:	~35.8kDa.
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	20 tests/100µl; 100 tests/500µl.
PURITY:	≥98% (SDS-PAGE, HPLC)
FORMULATION:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Do not freeze. Protect from light.

Product Description

APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:

- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂;
filtered through 0.2µm pore filter);
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml
- Take 195µl cell suspension and add 5µl Annexin V-FITC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)