ALEXIS Biochemicals: RGMA, Soluble (human) (recombinant)

ALX-522-103

Revised 19-Aug-08

RGMA, Soluble (human) (recombinant)
SYNONYMS	Repulsive Guidance Molecule A, Soluble (human) (recombinant) RGM Domain Family Member A, Soluble (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Repulsive Guidance Molecules [RGMs] & Neogenin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-103-2010		2x10 µg	240.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. Human RGMA (aa 48-422) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Specific Literature References

The Neogenin Intracellular Domain Regulates Gene Transcription via Nuclear Translocation: D. Goldschneider, et al.; Mol. Cell. Biol. 28, 4068 (2008) Abstract

General Information

The RGMA is an GPI-anchored protein involved in the axon guidance and is predominantly expressed in the developing and adult central nervous system in mouse. RGMA inhibits mammalian CNS neurite outgrowth. The protein acts as co-receptor of the bone morphogenetic protein. The mature RGMA protein is proteolytically processed and is glycosylated.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q96B86: Repulsive Guidance Molecule A (human) (Precursor) FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Repulsive guidance molecule (RGMa), a DRAGON homologue, is a bone morphogenetic protein co-receptor: J.L. Babitt, et al.; J. Biol. Chem. 280, 29820 (2005) Abstract; Full Text
RGMa inhibition promotes axonal growth and recovery after spinal cord injury: K. Hata, et al.; J. Cell Biol. 173, 47 (2006) Abstract

ALX-210-937	Polyclonal Antibody to RGMA (human) (AT115)
ALX-522-134	RGMA, Soluble (mouse) (recombinant)