ALEXIS Biochemicals: Thiolutin

ALX-380-200

Revised 31-Jul-08

Thiolutin
SYNONYMS	6-Acetamido-4-methyl-1,2-dithiolo(4,3-b)pyrrol-5(4H)-one Farcinicine Acetopyrrothine
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Antifungal

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-200-M001		1 mg	60.00 USD
ALX-380-200-M005		5 mg	240.00 USD

Product Specification

FORMULA:	C₈H₈N₂O₂S₂
MW:	228.3
CAS NUMBER:	87-11-6
MERCK INDEX:	14: 9342
RTECS:	JP1355000
SOURCE/HOST:	Isolated from Streptomyces luteosporeus.
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow powder.
SOLUBILITY:	Soluble in DMSO (1mg/ml); very slightly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	VERY TOXIC.

Product Description

Sulfur-containing antibiotic. Potent inhibitor of bacterial and yeast RNA polymerases. Inhibits mRNA chain elongation. Inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin (IC₅₀=0.83µM) and thus suppresses tumor cell-induced angiogenesis in vivo. Inhibits microbiological growth in beer. Active against soil borne pathogens.

Product Specific Literature References

W.D. Celmer, et al.; JACS 74, 6304 (1952)
W.D. Celmer & I.A. Solomons; JACS 77, 2861 (1955)
Inhibition of yeast ribonucleic acid polymerases by thiolutin: D.J. Tipper; J. Bacteriol. 116, 245 (1973) Abstract; Full Text
Inhibition of messenger ribonucleic acid synthesis in Escherichia coli by thiolutin: G.G. Khachatourians and D.J. Tipper; J. Bacteriol. 119, 795 (1974) Abstract; Full Text
In vivo effect of thiolutin on cell growth and macromolecular synthesis in Escherichia coli: G.G. Khachatourians and D.J. Tipper; Antimicrob. Agents Chemother. 6, 304 (1974) Abstract; Full Text
Cell wall synthesis regulation in Saccharomyces cerevisiae. Effect of RNA and protein inhibition: M.V. Elorza, et al.; Biochim. Biophys. Acta 454, 263 (1976) Abstract
P.R. Deb & B.K. Dutta; Curr. Sci. 53, 659 (1984)
The yeast heat shock response is induced by conversion of cells to spheroplasts and by potent transcriptional inhibitors: C.C. Adams and D.S. Gross; J. Bacteriol. 173, 7429 (1991) Abstract; Full Text
Thiolutin, an inhibitor of HUVEC adhesion to vitronectin, reduces paxillin in HUVECs and suppresses tumor cell-induced angiogenesis: K. Minamiguchi, et al.; Int. J. Cancer 93, 307 (2001) Abstract
Genome-wide analysis of mRNA stability using transcription inhibitors and microarrays reveals posttranscriptional control of ribosome biogenesis factors: J. Grigull, et al.; Mol. Cell. Biol. 24, 5534 (2004) Abstract; Full Text
Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor: B.W. Kebaara, et al.; Genome 49, 894 (2006) Abstract