ALEXIS Biochemicals: CD40 (human):COMP (human) (recombinant)

ALX-522-064

Revised 05-Apr-06

CD40 (human):COMP (human) (recombinant)
SYNONYMS	TNFRSF 5 (human):COMP (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD40 & CD40L [CD154]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-064-C010		10 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
MW:	~36kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human CD40 (aa 1-193) is fused to a FLAG^®-tag at the N-terminus and to human cartilage oligomeric matrix protein (COMP, aa 32-80) at the C-terminus.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human CD40L (CD154).
BIOLOGICAL ACTIVITY:	Potent blocker of mouse CD40L-dependent B cell proliferation without the requirement of any crosslinking reagent (enhancer).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Description

First non-antibody blocker of CD40L signalling

Product Specific Literature References

Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract

General Information

COMP (Cartilage Oligomeric Matrix Protein) also designated thrombospondin 5 (TSP 5) is an oligomeric protein synthesized by chondrocytes in all type of cartilage. It belongs to the thrombospondin family of extracellular proteins. It is a pentameric molecule forming a structure similar to a bouquet of flowers. It may have storage and delivery function for hydrophobic cell-signalling molecules such as vitamin D. COMP is a calcium-binding protein composed of 5 identical disulfide-linked subunits of 755 amino acids each with a high percentage of acidic residues (30%). The molecular weight of the homopentamer is 524 kDa, as determined by sedimentation equilibrium ultracentrifugation. Each subunit is composed of an N-terminal coiled-coil domain (unique to COMP), four epidermal growth factor (EGF)-type repeats, followed by eight calmodulin-like repeats and a globular C-terminal domain. The C-terminal domain binds to collagens I, II and IX. Chimeras composed of proteins fused to the pentamerization domain of COMP (aa 32-75) show an increased avidity compared to the protein alone.
BACKGROUND/TECHNICAL INFORMATION	FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Proteins • Recombinant Proteins/Fusion Proteins

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