ALEXIS Biochemicals: Monoclonal Antibody to RNase L (human) (2E9)

ALX-804-581

Revised 19-Feb-07

Monoclonal Antibody to RNase L (human) (2E9)
SYNONYMS	anti-RNase L (human) MAb (2E9) anti-Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-dependent) (human) MAb (2E9) anti-2-5A-Dependent RNase (human) MAb (2E9) anti-Ribonuclease 4 (human) MAb (2E9)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Regulation / Transcription Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-581-C100		100 µg	190.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	2E9
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN:	Recombinant human RNase L.
SPECIFICITY:	Recognizes human RNase L. Detects a band of ~83kDa by Western blot. Does not cross-react with mouse RNase L.
APPLICATION:	ELISA Western Blot (Alkaline phosphatase/ECL detection) Immunoprecipitation Immunocytochemistry Immunohistochemistry (paraffin sections)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

2-5A-dependent RNase molecules dimerize during activation by 2-5A: B. Dong & R.H. Silverman; J. Biol. Chem. 270, 4133 (1995) Abstract; Full Text
Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis: L. Wang, et al.; Clin. Cancer Res. 1, 1421 (1995) Abstract
Alternative function of a protein kinase homology domain in 2', 5'-oligoadenylate dependent RNase L: B. Dong & R.H. Silverman; Nucleic Acids Res. 27, 439 (1999) Abstract
Germline mutations in the ribonuclease L gene in families showing linkage with HPC1: J. Carpten, et al.; Nat. Genet. 30, 181 (2002) Abstract
An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors: B. Dong, et al.; PNAS 104, 1655 (2007) Abstract; Full Text

General Information

RNase L is an endoribonuclease regulated by 2-5A synthetase (2-5A). It is a 741 aa cytoplasmic and mitochondrial protein in human. RNase L functions in the antiviral and antiproliferative activities of interferons (IFN) and in multiple apoptotic pathways, standing in the first line of the innate cellular defense mechanism induced by the IFN pathway.
In its native state, the N-terminal half of RNase L functions as a repressor of the ribonuclease domain in the C-terminal half. Upon binding with high affinity to 2-5A molecules, the inactive monomeric form of RNase L is induced to dimerize into its catalytically active form.
Since mutations in RNase L relate to an increase incidence of prostate cancer, the activation of RNase L directly or indirectly suppresses prostate tumorigenesis and/or metastasis. The 2-5A/RNase L pathway is implicated in mediating apoptosis in response to viral infections and to several types of external stimuli. The fungal ribonuclease, α-sarcin, the RNA modyfing enzyme, ricin A chain, and UV light each lead to ribotoxic stress responses involving damage to the 3-end of the large ribosomal RNA. These treatments activate c-jun N-terminal kinases (JNKs). JNK activation in response to UV irradiation has been linked to apoptosis through Bax (requires caspase 3 activity, appearance of cytosolic cytochrome C indicating involvement of mitochondria).

General Literature References

Implications for RNase L in prostate cancer biology: R.H. Silverman; Biochemistry 42, 1805 (2003), Review Abstract
Effects of RNase L mutations associated with prostate cancer on apoptosis induced by 2',5'-oligoadenylates: Y. Xiang, et al.; Cancer Res. 63, 6795 (2003) Abstract

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