ALEXIS Biochemicals: Monoclonal Antibody to Dnmt3b (52A1018)

ALX-804-233

Revised 31-May-07

Monoclonal Antibody to Dnmt3b (52A1018)
SYNONYMS	anti-DNA Methyltransferase-3b MAb (52A1018)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA & RNA Methyl & Alkyl Transferases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-233-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
CLONE:	52A1018
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:	Recombinant mouse Dnmt3b (DNA methyltransferase-3b).
SPECIFICITY:	Recognizes human and mouse Dnmt3b. Detects a band of ~100kDa by Western blot. Does not cross-react with Dnmt3a.
APPLICATION:	Immunocytochemistry (1-2µg/ml) Immunohistochemistry (see [6] and [7]) Immunoprecipitation (1-2µg/ml); Chromatin Immunoprecipitation (see [5]) Western Blot (2-4µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.

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Product Specific Literature References

[1] The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet. 32, 393 (2002) Abstract
[2] Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res. 63, 6110 (2003) Abstract
[3] Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation: D.J. Weisenberger, et al.; Mol. Cancer Res. 2, 62 (2004) Abstract; Full Text
[4] Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature 431, 211 (2004) Abstract
[5] Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004) Abstract
[6] An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett. 226, 77 (2005) Abstract
[7] Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development: J.L. MacDonald, et al.; Dev. Biol. 288, 461 (2005) Abstract; Full Text
[8] Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein: D.L. Carlone, et al.; Mol. Cell. Biol. 25, 4881 (2005) Abstract; Full Text

General Information

DNA methylation is an important regulator of genetic information, shutting down gene expression through the addition of methyl groups to cytosine (C) bases in the DNA. The establishment of DNA methylation patterns requires de novo methylation that occurs predominantly during early development and gametogenesis. DNA methyltransferase-1 (Dnmt1) "maintains" C methylation after DNA replication by methylating the daughter DNA strand using the methylation pattern of the parental strand as a template. Dnmt3a and Dnmt3b are essential for the de novo methylation. They show nonoverlapping functions in development, with Dnmt3b specifically required for methylation of centromeric minor satellite repeats. Both Dnmt3a and Dnmt3b expression is elevated in several tumor cell lines comparable to Dnmt1. It has also been suggested that mutations of Dnmt3b are responsible for the ICF immunodeficiency syndrome.

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