ALEXIS Biochemicals: Monoclonal Antibody to Calpain (156)

ALX-804-053

Revised 11-Jan-05

Monoclonal Antibody to Calpain (156)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calpains / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-053-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Others
CLONE:	156
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:	Purified 28kDa calpain subunit from bovine skeletal muscle.
SPECIFICITY:	Recognizes human and bovine 28kDa calpain subunit. Detects a band of ~28kDa by Western blot. Does not cross-react with the human 80kDa m-calpain subunit.
APPLICATION:	Western Blot (1:1'000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (µ-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
µ- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Product Specific Literature References

Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains: J. Cong, et al.; J. Biol. Chem. 268, 25740 (1993) Abstract; Full Text

Further Categories Containing This Product:

Monoclonal Antibodies

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