© 2008 Alexis Corporation
ALX-201-059
Revised 21-Jul-08
Caspase-3 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-059-U025
25 U
119.00 USD
ALX-201-059-U100
100 U
273.00 USD
Product Specification
EC:
3.4.22.56
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
300’000-400’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA (Prod. No.
ALX-260-033
) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-216
or
ALX-850-215
).
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-
p
NA (Prod. No.
ALX-260-033
) and Ac-DEVD-AFC (Prod. No.
ALX-260-032
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.
BACKGROUND/TECHNICAL INFORMATION
Swiss Prot link:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link:
Caspase-3 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins/Fusion Proteins
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