ALEXIS Biochemicals: Monoclonal Antibody to globo H (MBr1)

ALX-804-550

Revised 20-Aug-08

Monoclonal Antibody to globo H (MBr1)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-550-C050		50 µg	200.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat Dog Others
CLONE:	MBr1
ISOTYPE:	Mouse IgM
CONCENTRATION:	0.5mg/ml.
PURITY:	≥90% (SDS-PAGE)
PURITY DETAIL:	Purified from concentrated hybridoma cell culture supernatant by thiophilic adsorption chromatography.
FORMULATION:	Liquid. In 0.5M PBS, pH 8.0, containing 150mM sodium chloride. Contains no sodium azide.
IMMUNOGEN:	Membrane preparation from the human breast carcinoma cell line MCF7.
SPECIFICITY:	Recognizes human, rat, dog and cat membrane bound glycoconjugates (glycoproteins, mucins and glycolipids) expressing the globo H or its tetrasaccharide terminus. Recognizes an epitope consisting of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal. Does not cross-react with mouse. Cross-reactivity with other species has not been tested.
APPLICATION:	Flow Cytometry (1-10µg/ml) Immunocytochemistry (1-10µg/ml) Immunohistochemsitry (frozen sections (1-10µg/ml); paraffin sections (1-10µg/ml, no need of antigen retrieval)) Western Blot (1-10µg/ml, for glycoproteins)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Do not freeze/thaw.

Product Description

The murine monoclonal antibody to globo H (clone MBr1) is raised against the breast cancer cell line MCF7 [1] and recognizes a saccharidic epitope (CaMBr1) overexpressed on a high percentage of human breast, ovary lung and prostate carcinomas [1, 2, 3]. This antigen was originally identified on the immunogen as a globo-series glycosphingolipid with a H-like determinant at its terminus (globo-H), identical to GL6 isolated from human teratocarcinoma [4]. MAb to MBr1 was also found to be reactive with different glycoconjugates (glycoproteins, mucins and glycolipids) in breast and small cell lung carcinoma [12, 15, 16]. Retrospective as well as prospective studies suggest that CaMBr1 expression is associated with tumor aggressiveness and survival outcome in breast and small cell lung cancer [15, 16] and that poor prognosis is more frequently associated with CaMBr1 expression on glycoproteins. The epitope recognized by the MAb to MBr1 consists of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal [18], available as a synthetic oligosaccharide [17, 23] exploitable for the development of new anti-cancer vaccines. Carbohydrate antigens expressed at the cell surface of human cancer cells hold promise as targets for active immunization with vaccines.
CaMBR1 is also expressed on some normal tissues [3], and its expression restricted tissue distribution makes it a suitable target for oncologic clinical applications. In particular the MAb to MBr1 can be succesfully used for immunocytochemical detection of micrometastatic cells [14].

Product Specific Literature References

[1] Generation of monoclonal antibodies reacting with normal and cancer cells of human breast: S. Menard, et al.; Cancer Res. 43, 1295 (1983) Abstract
[2] Immunochemical analysis of the determinant recognized by a monoclonal antibody (MBr1) which specifically binds to human mammary epithelial cells: S. Canevari, et al.; Cancer Res. 43, 1301 (1983) Abstract
[3] Reactivity of a monoclonal antibody with tissues and tumors from the human breast. Immunohistochemical localization of a new antigen and clinicopathologic correlations: R. Mariani-Costantini, et al.; Am. J. Pathol. 115, 47 (1984) Abstract
[4] Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland: E.G. Bremer, et al.; J. Biol. Chem. 259, 14773 (1984) Abstract; Full Text
[5] Immunohistochemical reactivity of a monoclonal antibody prepared against human breast carcinoma: R. Mariani-Costantini, et al.; Virchows Arch. A Pathol. Anat. Histopathol. 402, 389 (1984) Abstract
[6] Carcinoma of the breast and of the ovary as a model for the application of monoclonal antibodies to diagnostic pathology: F. Rilke, et al.; Monogr. Pathol. 27, 206 (1986) Abstract
[7] Association of monoclonal antibody-recognized antigen with steroid receptors in gynecologic tumors: K.C. Wang, et al.; Cancer Invest. 6, 151 (1988) Abstract
[8] Monoclonal antibody detection of carcinoma cells in bone marrow biopsy specimens from breast cancer patients: G. Porro, et al.; Cancer 61, 2407 (1988) Abstract
[9] Type 4 chain H expression by bile ductules and hepatocytes in cirrhosis: Y. Okada, et al.; J. Pathol. 157, 329 (1989) Abstract
[10] Relationship between CaMBr1 expression and tumor progression in small cell lung carcinomas: S. Martignone, et al.; Tumori 75, 373 (1989) Abstract
[11] In vivo and in vitro growth of SCLC cells derived from biopsies: S. Giani, et al.; Tumori 75, 570 (1989) Abstract
[12] Chapter 17: Immunoblotting detection of carbohydrate epitopes in glycolipids and glycoproteins of tumoral origin: D. Miotti, et al.; Gangliosides and Cancer (Weinheim, VCH Verlagsgesellschaft) 169 (1989)
[13] Expression of two antigens defined by monoclonal antibodies in normal, benign and malignant human mammary tissues: F. Perrone, et al.; Tumori 76, 525 (1990) Abstract
[14] Use of monoclonal antibody MBr1 to detect micrometastases in bone marrow specimens of breast cancer patients: B. Salvadori, et al.; Eur. J. Cancer 26, 865 (1990) Abstract
[15] Prognostic significance of the CaMBr1 antigen on breast carcinoma: relevance of the type of recognised glycoconjugate: F. Perrone, et al.; Eur. J. Cancer 29A, 2113 (1993) Abstract
[16] Study of the expression and function of the tumour-associated antigen CaMBr1 in small cell lung carcinomas: S. Martignone, et al.; Eur. J. Cancer 29A, 2020 (1993) Abstract
[17] Oligosaccharides related to tumor-associate antigens, Part 3: L. Lay, et al.; Helv. Chim. Acta 78, 533 (1995)
[18] In vitro mimicry of CaMBr1 tumor-associated antigen by synthetic oligosaccharides: E. Adobati, et al.; Glycobiology 7, 173 (1997) Abstract
[19] Use of monoclonal antibody MBr1 to detect micrometastases in bone marrow specimens of breast cancer patients: B. Salvadori, et al.; Eur. J. Cancer 26, 865 (1990) Abstract
[20] Synthesis, modeling and binding affinity of an ester analogue of the terminal trisaccharide of the tumor-associated antigen globo-H: S. Canevari, et al.; Tetrahedron 55, 1469 (1999) Abstract; Full Text
[21] Expression profile of saccharide epitope CaMBr1 in normal and neoplastic tissue from dogs, cats, and rats: implication for the development of human-derived cancer vaccines: E. Adobati, et al.; Histochem. J. 31, 729 (1999) Abstract
[22] Role for alpha1,2-fucosyltransferase and histo-blood group antigen H type 2 in resistance of rat colon carcinoma cells to 5-fluorouracil: S. Cordel, et al.; Int. J. Cancer 85, 142 (2000) Abstract
[23] Improvement of the synthesis of immunological carbohydrate vaccines containing the tumour associate antigen CaMBr1: L. Lay, et al.; Eur. J. Org. Chem. 22, 4331 (2001) Abstract
[24] Carbohydrate microarray for profiling the antibodies interacting with Globo H tumor antigen: C.Y. Huang, et al.; PNAS 103, 15 (2006) Abstract

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