ALEXIS Biochemicals: Monoclonal Antibody to GCDFP-15 (human) (D6)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-801-051-L001		1 ml	670.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	D6
ISOTYPE:	Mouse IgG2a
FORMULATION:	Liquid. In 0.01M PBS containing 1% BSA and 0.1% sodium azide .
IMMUNOGEN:	GCDFP-15 (gross cystic disease fluid protein-15; BRST-2).
SPECIFICITY:	Recognizes human GCDFP-15.
APPLICATION:	Immunohistochemistry (paraffin sections): Formalin-fixed, paraffin-embedded tissues; pre-treatment not required; suggested dilution: ≥1:40 for biotin based detection. For optimal staining, incubate primary antibody 20-60 minutes at room temperature). Optimal dilution must be determined individually for each application. Note: This antibody is heat sensitive. Paraffin sections that have been heat fixed at 60°C may lose antibody binding site.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Immunohistochemistry of a gross cystic disease fluid protein (GCDFP-15) of the breast. A marker of apocrine epithelium and breast carcinomas with apocrine features: G. Mazoujian, et al.; Am. J. Pathol. 110, 105 (1983) Abstract
Extramammary Paget's disease--evidence for an apocrine origin. An immunoperoxidase study of gross cystic disease fluid protein-15, carcinoembryonic antigen, and keratin proteins: G. Mazoujian, et al.; Am. J. Surg. Pathol. 8, 43 (1984) Abstract
Gross cystic disease fluid protein-15 as a marker for breast cancer: immunohistochemical analysis of 690 human neoplasms and comparison with alpha-lactalbumin: M.R. Wick, et al.; Hum. Pathol. 20, 281 (1989) Abstract
Expression of androgen receptor and prostatic specific markers in salivary duct carcinoma: an immunohistochemical analysis of 13 cases and review of the literature: C.Y. Fan, et al.; Am. J. Surg. Pathol. 24, 579 (2000) Abstract

General Information

Gross cystic disease is a common premenopausal disorder in which gross cysts are the predominant pathologic lesion. It is characterized by production of a fluid secretion which accumulates in the breast cysts. Gross cystic disease fluid is a pathologic secretion from breast composed of several glycoproteins, including a unique 15kDa monomer protein, GCDFP-15. The cells within the body that produce GCDFP-15 appear to be restricted primarily to those with apocrine function. Studies have found GCDFP-15 to be a highly specific and sensitive marker for breast cancer.
GCDFP-15 is expressed by apocrine sweat glands, eccrine glands (variable), minor salivary glands, bronchial glands, metaplasmic epithelium of the breast, benign sweat gland tumors of the skin and the serous cells of the submandibular gland. Breast carcinomas (primary and metastatic lesions) with apocrine features express GCDFP-15. GCDFP-15 is positive in extramammary Paget's Disease. Other tumors tested are negative.

General Literature References

C.D. Haagensen; Disease of the breast. 3rd edition. W.B. Saunders Co. Philadelphia, PA. 250 (1986)

Further Categories Containing This Product:

Monoclonal Antibodies