ALEXIS Biochemicals: Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)

ALX-804-111

Revised 15-Oct-07

Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)
SYNONYMS	anti-Glc-NAc (O-linked) MAb (RL2)
PRODUCT LINE	Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY	Protein Glycosylation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-111-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
CLONE:	RL2
ISOTYPE:	Mouse IgG1
CONCENTRATION:	2mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Pore complex-lamina fraction purified from rat liver nuclear envelopes.
SPECIFICITY:	Recognizes human, mouse and rat nuclear pore complex (NPC), cytoplasmic and intracellular O-GlcNAc glycosylated proteins. Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot.
APPLICATION:	Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections (1:200)) Immunoprecipitation Western Blot (1:1’000) Note: Do not use with diluents containing glycosylated proteins.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Monoclonal antibodies identify a group of nuclear pore complex glycoproteins: C.M. Snow, et al.; J. Cell. Biol. 104, 1143 (1987) Abstract
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1: G. Majumdar, et al.; Diabetes 53, 3184 (2004) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC.

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Monoclonal Antibodies

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