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ALX-804-259 Revised 21-Oct-08
Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-259-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 7A7
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to aa 35-50 of human β-catenin.
SPECIFICITY: Recognizes human, mouse, rat (weak) and dog cytoplasmic dephospho β-catenin.
APPLICATION: Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-022).
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 

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