© 2008 Alexis Corporation
ALX-521-008
Revised 17-Oct-06
Nerve Growth Factor 7S (mouse)
SYNONYMS
NGF 7S (mouse)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Nerve Growth Factors [NGF] & Receptors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-521-008-C100
100 µg
245.00 USD
Product Specification
MW:
~130kDa.
SOURCE/HOST:
Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol.
4
, 79 (1984).
QUANTITY:
Determined by Lowry and Pierce method.
PURITY:
≥97% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation.
BIOLOGICAL ACTIVITY:
Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used
in vitro
for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium (See [2]).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
[1] O.H. Lowry, et al.; J. Biol. Chem.
193
, 265 (1951)
[2]
The isolation of the mouse nerve growth factor protein in a high molecular weight form:
S. Varon, et al.; Biochemistry
6
, 2202 (1967)
Abstract
[3]
The changing scene of neurotrophic factors:
H. Thoenen; TINS
14
, 165 (1991), (Review)
Abstract
[4]
Towards a comprehensive understanding of the trophic support of motoneurons:
H. Thoenen, et al.; C. R. Acad. Sci. III
316
, 1158 (1993), (Review)
Abstract
[5]
Neurotrophic factors enter the clinic:
M. Barinaga; Science
264
, 772 (1994), (Review)
Abstract
[6]
Neurobiology. Tracking neurotrophin function:
A.M. Davies; Nature
368
, 193 (1994), (Review)
Abstract
[7]
Role of neurotrophins in mouse neuronal development:
R. Klein; FASEB J.
8
, 738 (1994), (Review)
Abstract
[8]
The neurotrophins and their receptors: structure, function, and neuropathology:
L.M. Maness, et al.; Neurosci. & Biobehav. Rev.
18
, 143 (1994), (Review)
Abstract
[9]
Neurotrophic factors: two are better than one:
R. Nishi; Science
265
, 1052 (1994), (Review)
Abstract
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