ALEXIS Biochemicals: Sp3 Protein (human) (recombinant) (HA)

ALX-201-107

Revised 17-Nov-06

Sp3 Protein (human) (recombinant) (HA)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Sp Protein Family / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-107-R050		50 µl	377.00 USD

Product Specification

SOURCE/HOST:	Produced in insect cells (Schneider SL2 cells). Human Sp3 is fused to a HA- and a FLAG^®-tag (H. Braun and G. Suske; Biotechniques 26, 1038 (1999)).
QUANTITY:	Sufficient for performing at least 25 protein-DNA interaction experiments in 10 mM HEPES (pH 7.9), 0.1 mM EDTA, 8.5 % Glycerol, 170 mM KCl, 1 µM ZnSO₄, 37.5 µg/ml Poly dIdC, 10 mM DTT, and 1 mg/ml BSA, analyzed by gel shift assay.
PURITY:	~50% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS-Cl, pH 7.9, containing 150mM KCl, 0.2mM EDTA and 20% glycerol.
SPECIFICITY:	Binds specifically to an oligonucleotide with the sequence: GGG GCG GGG.
SPECIFIC ACTIVITY:	>0.5 BFU/µl (band forming units) 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
APPLICATION:	EMSA promoter characterization, in vitro transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Specific Literature References

The Sp-family of transcription factors: G. Suske; Gene 238, 291 (1999), (Review) Abstract
Vectors for inducible expression of dual epitope-tagged proteins in insect cells: H. Braun & G. Suske; Biotechniques 26, 1038 (1999) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

FLAG is a registered trademark of Sigma-Aldrich Co.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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