ALEXIS Biochemicals: FasL, Soluble (human) (recombinant)

ALX-522-001

Revised 10-Oct-08

FasL, Soluble (human) (recombinant)
SYNONYMS	APO-1L, Soluble (human) (recombinant) CD95L, Soluble (human) (recombinant) CD178, Soluble (human) (recombinant) TNFSF 6, Soluble (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	FasL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-001-C010		10 µg	200.00 USD
ALX-522-001-3010	SuperPack	3x10 µg	410.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
MW:	~32kDa (non-glycosylated), ~35kDa (glycosylated) (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human, mouse and rat Fas (CD95; APO-1).
BIOLOGICAL ACTIVITY:	Kills Fas-sensitive cells at concentrations of >50ng/ml. Cross-linking enhancer (see Set Prod. No. ALX-850-014) increases the activity of rhsFasL approx. 50-fold. Attention: Results using sFasL may differ from those obtained with agonistic antibodies! (see [1]).
SPECIFIC ACTIVITY:	ED₅₀: 50ng/ml (A20 cells)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

[1] Agonist antibody and Fas ligand mediate different sensitivity to death in the signaling pathways of Fas and cytoplasmic mutants: A.R. Thilenius, et al.; Eur. J. Immunol. 27, 1108 (1997) Abstract
Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997) Abstract; Full Text
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001) Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001) Abstract
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol. 166, 6964 (2001) Abstract
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001) Abstract; Full Text
BCR engagement induces Fas resistance in primary B cells in the absence of functional Bruton's tyrosine kinase: J.R. Tumang, et al.; J. Immunol. 168, 2712 (2002) Abstract
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003) Abstract; Full Text
Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins: a novel mechanism of T-cell depletion: M. C. Michallet, et al.; Blood 102, 3719 (2004) Abstract
The anti-apoptotic factor Bcl-2 can functionally substitute for the B cell survival but not for the marginal zone B cell differentiation activity of BAFF: A. Tardivel, et al.; Eur. J. Immunol. 34, 509 (2004) Abstract
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005) Abstract; Full Text
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P48023: FasL (human)
AfCS Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Proteins • Recombinant Proteins / Fusion Proteins