ALEXIS Biochemicals: FLUO 3 . pentaammonium salt

ALX-620-002

Revised 04-Feb-05

FLUO 3 . pentaammonium salt
SYNONYMS	1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid . 5NH₃
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Chelators / Caged Calcium Chelators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-002-M001		1 mg	210.00 USD

Product Specification

FORMULA:	C₃₆H₃₀Cl₂N₂O₁₃. 5 NH₃
MW:	769.6 . 85.2
CAS NUMBER:	134907-84-9
PURITY:	≥75%
APPEARANCE:	Reddish brown powder.
SOLUBILITY:	Soluble in alkaline solution.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Ca²⁺ chelator and indicator. Designed to operate at longer wavelengths of excitation. Among the advantages that it offers are the suitability of argon laser sources for excitations, less interference from endogenous autofluorescence, and the possibility of using other fluorescent probes simultaneously. As with QUIN 2 (Prod. No. ALX-620-006), this chelator responds to Ca²⁺without significant changes in excitation or emission maxima hence ratioing techniques cannot be used for the estimation of Ca²⁺concentrations. A unique feature of FLUO 3 is that both the acetoxymethyl ester (FLUO 3/AM) and the uncomplexed chelator (FLUO 3) are only weakly fluorescent, but chelation with Ca²⁺produces a strong enhancement of fluorescence.

Product Specific Literature References

New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures: R.Y. Tsien; Biochemistry 19, 2396 (1980) Abstract
Fluorescence measurement and photochemical manipulation of cytosolic free calcium: R.Y. Tsien; TINS 11, 419 (1988) Abstract
Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescence: M. Eberhard & P. Erne; BBRC 163, 309 (1989) Abstract
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores: A. Minta, et al.; J. Biol. Chem. 264, 8171 (1989) Abstract; Full Text
Photochemically generated cytosolic calcium pulses and their detection by fluo-3: J.P.Y. Kao, et al.; J. Biol. Chem. 264, 8179 (1989) Abstract; Full Text
Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+: J.E. Merritt, et al.; Biochem. J. 269, 513 (1990) Abstract
Improved method for measuring intracellular Ca++ with fluo-3: G.T. Rijkers, et al.; Cytometry 11, 923 (1990) Abstract
Control of mitochondrial matrix calcium: studies using fluo-3 as a fluorescent calcium indicator: A. Saavedra-Molina, et al.; BBRC 167, 148 (1990) Abstract
Effects of photolabile calcium chelators on fluorescent calcium indicators: R.S. Zucker; Cell Calcium 13, 29 (1992) Abstract

Further Categories Containing This Product:

MDR Other Products • Calcium Indicators / Probes • Dyes / Stains / Fluorescent Probes / Fluorescent Labels

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