ALEXIS Biochemicals: Polyclonal Antibody to VE-cadherin

ALX-210-232

Revised 07-Oct-08

Polyclonal Antibody to VE-cadherin
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Cadherin Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-232-C100	Purified	100 µg	308.00 USD
ALX-210-232B-T100	Biotin	100 tests	355.00 USD
ALX-210-232F-T100	FITC	100 tests	355.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
SOURCE/HOST:	From rabbit.
PURITY DETAIL:	Affinity purified using recombinant VE-cadherin fragments.
FORMULATION:	Unconjugated PAb: Liquid. 1mg/ml in PBS. Biotin- and FITC-conjugates: Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN:	Recombinant human VE-cadherin (aa 1-258).
SPECIFICITY:	Recognizes human, mouse, rat and bovine VE-cadherin.
APPLICATION:	Confocal Microscopy [1] ELISA Flow Cytometry Immunocytochemistry Immunohistochemistry: Frozen sections (formalin or acetone-fixed sections) (1µg/ml); not recommended for paraffin sections. Western Blot [1] (see also protocol modifications) Functional Application: Interferes with VE-cadherin adhesion (reversible endothelium disruption) This effect mimics the transmigration of leukocytes or cancer cells through endothelium. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. Keep Biotin- and FITC-conjugated formats at +4°C. Protect from light.

Product Specific Literature References

[1] Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin: D. Gulino, et al.; J. Biol. Chem. 273, 29786 (1998) Abstract; Full Text
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function: S. Iyer, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1143 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Protocol Modifications for Western blot:
Protocol described in [1]; To improve the detection of VE cadherin, the following modifications are recommended:
Use PAb to VE-cadherin (Prod. No. ALX-210-232) diluted to 1µg/ml in PBS containing 0.01% Thimerosal (concentration must be adjusted according to the batch used). The solution can be used several times. Incubate overnight at room temperature under stirring. Rinsing: 6 x 5 minutes plus 10 minutes at room temperature.
Secondary antibody: Biotinylated goat anti-rabbit antibody (VC-BA-1000) diluted at 1:5’000 in 1% gelatin. Avoid exposure to light. Incubation 1h 30 at room temperature under stirring. Rinse in PBS 0.05% Tween 20 for 50 minutes at room temperature under stirring.
Signal amplification using Strept ABComplex/ HRP (Dako): Follow the protocol according to Dako instructions. Dilute Strept ABComplex/HRP to 1:5’000. Avoid exposure to light. Incubate 1h at room temperature while stirring. Rinse in PBS 0.05% Tween 20, 50 minutes at room temperature while stirring. Rinse rapidly in PBS.
Detection using the Supersignal Kit Pierce: Incubate the membrane 10 minutes with the solution under red light. Membrane is then packed in Plastic wrap and put in contact with the film. This method should allow for the detection of very small amounts of VE cadherin (less than 1ng/well).

Further Categories Containing This Product:

Angiogenesis Other Products • Polyclonal Antibodies

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