ALEXIS Biochemicals: Pam3Cys-Ser-(Lys)4 . trihydrochloride

ALX-165-066

Revised 08-Mar-07

Pam3Cys-Ser-(Lys)4 . trihydrochloride
SYNONYMS	Pam₃CSK₄ . 3HCl (S)-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys- (S)-Ser-(S)-Lys₄-OH . 3HCl
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Agonists Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-165-066-M002		2 mg	260.00 USD

Product Specification

FORMULA:	C₈₁H₁₅₆N₁₀O₁₃S . 3HCl
MW:	1510.3 . 109.4
CAS NUMBER:	112208-00-1
CONCENTRATION:	1mg/ml after reconstitution.
FORMULATION:	Lyophilized.
SOLUBILITY:	Soluble in water, saline or aqueous buffers at pH <7.5.
RECONSTITUTION:	Reconstitute in 2ml distilled water. Use a homogeniser or sonicator to prepare a homogenous solution.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Description

Selective agonist of Toll-like receptor 1 (TLR1) complexed with TLR2. Cell permeable, water soluble synthetic cationic lipohexapeptide analog of the immunologically active N-terminal portion of bacterial lipoprotein that potently activates monocytes and macrophages. Activates/represses an array of at least 140 genes partly involved in signal transduction and regulation of the immune response.

Product Specific Literature References

Induction of tumor cytotoxicity in murine bone marrow-derived macrophages by two synthetic lipopeptide analogues: P. Hoffmann, et al.; Biol. Chem. Hoppe Seyler 370, 575 (1989) Abstract
Lipopeptide derivatives of bacterial lipoprotein constitute potent immune adjuvants combined with or covalently coupled to antigen or hapten: A. Reitermann, et al.; Biol. Chem. Hoppe Seyler 370, 343 (1989) Abstract
Activation of superoxide formation and lysozyme release in human neutrophils by the synthetic lipopeptide Pam3Cys-Ser-(Lys)4. Involvement of guanine-nucleotide-binding proteins and synergism with chemotactic peptides: R. Seifert, et al.; Biochem. J. 267, 795 (1990) Abstract
Synthesis of novel immunologically active tripalmitoyl-S-glycerylcysteinyl lipopeptides as useful intermediates for immunogen preparations: J. Metzger, et al.; Int. J. Pept. Protein Res. 37, 46 (1991) Abstract
The influence of various adjuvants on antibody synthesis following immunization with an hapten: J. Kellner, et al.; Biol. Chem. Hoppe Seyler 373, 51 (1992) Abstract
Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells: S. Offermanns, et al.; Biochem. J. 282, 551 (1992) Abstract
Evidence for common mechanisms in the transcriptional control of type II nitric oxide synthase in isolated hepatocytes. Requirement of NF-kappaB activation after stimulation with bacterial cell wall products and phorbol esters: M.J. Diaz-Guerra, et al.; J. Biol. Chem. 271, 30114 (1996) Abstract; Full Text
Adjuvant lipopeptide interaction with model membranes: J. Gonzalez-Christen; Biochim. Biophys. Acta 1368, 97 (1998) Abstract
Alteration of the lateral organization of the plasma membrane of Chinese hamster ovary cells by synthetic lipopeptide, Pam3Cys-Ser-Lys4: I. Vergne & L. Cezanne; Eur. J. Biochem. 264, 369 (1999) Abstract; Full Text
Adjuvant effects of various lipopeptides and interferon-gamma on the humoral immune response of chickens: M.H. Erhard, et al.; Poult. Sci. 79, 1264 (2000) Abstract
Immunostimulation by the synthetic lipopeptide P3CSK4: TLR4-independent activation of the ERK1/2 signal transduction pathway in macrophages: M.R. Muller, et al.; Immunology 103, 49 (2001) Abstract
TLR4, but not TLR2, mediates IFN-beta-induced STAT1alpha/beta-dependent gene expression in macrophages: V. Toshchakov, et al.; Nat. Immunol. 3, 392 (2002) Abstract
The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood 109, 1507 (2007) Abstract