| SPECIES CROSSREACTIVITY: |
|
| SOURCE/HOST: |
From goat. |
| CONCENTRATION: |
1mg/ml. |
| PURITY DETAIL: |
Protein G-affinity purified. |
| FORMULATION: |
Liquid. In PBS, pH 7.4. |
| IMMUNOGEN: |
Recombinant human CD44var(v3-v10). |
| SPECIFICITY: |
Recognizes epitopes encoded by exons v3-v10 of human variant CD44. |
| APPLICATION: |
Immunohistochemistry: Recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedure or the three stage immunoperoxidase technique on acetone fixed frozen sections (50µg/ml). Use of biotinylated swine anti-goat, mouse, rabbit immunoglobulins is recommended. Blocking should be done with swine serum.
Paraffin-embedded tissue after microwave treatment (1x5 min.) (20µg/ml).
Western Blot (1:1’000)
Optimal conditions must be determined individually for each application. |
| SHIPPING: |
SHIPPED ON BLUE ICE |
| LONG TERM STORAGE: |
-20°C |
| HANDLING: |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
