| SPECIES CROSSREACTIVITY: |
|
| CLONE: |
RLEP 227 |
| ISOTYPE: |
Mouse IgG2a |
| CONCENTRATION: |
1mg/ml |
| FORMULATION: |
Liquid. In PBS, pH 7.4. Contains no preservatives. |
| IMMUNOGEN: |
Recombinant rat leptin. |
| SPECIFICITY: |
Recognizes both natural and recombinant rat leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with human or mouse leptin. |
| APPLICATION: |
ELISA (direct and indirect: 1:5'000-1:10'000).
Western Blot (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application. |
| SHIPPING: |
SHIPPED ON BLUE ICE |
| SHORT TERM STORAGE: |
+4°C |
| LONG TERM STORAGE: |
-20°C |
| HANDLING: |
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
